Identification and analysis of a factor relevant to the promotion of endometrial cancer

子宫内膜癌发生相关因素的鉴定与分析

• 批准号: 13671741
• 负责人: SUZUKI Masami
• 金额: $ 0.9万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671741/
• 关键词: endometrial cancer; extracted RNA; DNA chip method; estrogen induced gene; エストロゲン依存症; エストロゲン誘導体遺伝子; 子官内膜癌; 子宮内膜細胞増殖因子; DNAchip法; efp下流遺伝子の単離

Estrogen may play an important role also in functional regulation of bone metabolism besides cardiovascular system and central nervous system. Furthermore, estrogen having broad physiological function which in multiplication and canceration of mammary gland or endometrium for estrogen target organ is also well known. However these details are unknown. Endometrium easily influenced one of organ with estrogen. Estrogen is a risk-factor of playing the role central as breast cancer, endometrial cancer and endometrial hyperplasia. For clinical use studies, an estrogen supplement treatment positively performed for the prevention and the medical treatment purpose of osteoporosis or ischemic heart disease which are known as climacteric disturbance after a menopause. This research solved the mechanism related to cell multiplication and canceration of estrogen on the moleculer levels. The new estrogen induced gene was isolated from extracted RNA and searched for the endometrium growth factor.The rate of discovery of the oncogene in endometrial cancer was lowered and that relation with a new estrogen induced gene was not made clearly. Considering this experiment result, the method of isolated a new gene from RNA extracted using the DNA chip method rather than isolation of the gene by classical handmade method is efficient, and the analysis method by which sufficient result is expected is seemed.

除心血管系统和中枢神经系统以外，雌激素在骨代谢的功能调节中也可能发挥重要作用。此外，具有广泛生理功能的雌激素在雌激素靶器官的乳腺或子宫内膜的繁殖和癌症中也是众所周知的。但是这些细节是未知的。子宫内膜很容易用雌激素影响一个器官。雌激素是在乳腺癌，子宫内膜癌和子宫内膜增生中扮演中心角色的风险因素。为了临床使用研究，雌激素补充剂治疗对骨质疏松症或缺血性心脏病的预防和治疗目的进行了积极的治疗，被称为更年期后的最新障碍。这项研究解决了与分子水平上雌激素的细胞繁殖和癌症有关的机制。从提取的RNA中分离出新的雌激素诱导的基因并搜索子宫内膜生长因子。子宫内膜癌中癌基因的发现率降低了，并且与新的雌激素诱导基因的关系尚未清楚。考虑到该实验结果，从使用DNA芯片方法提取的RNA分离出新基因的方法是有效的，而不是通过经典手工方法隔离基因，并且似乎可以预期有足够结果的分析方法。