Research of the microleakage test in the adhesive restoration using the Confocal Laser Scanning Microscope

共焦激光扫描显微镜微渗漏测试研究

• 批准号: 13672014
• 负责人: MASEKI Toshio
• 金额: $ 1.54万
• 依托单位: The Nippon Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672014/
• 关键词: Confocal Laser Scanning Microscope; Sealing Property; Microleakage; Adhensive Materials; Dye Penetration Test; Fluorescence Image; Operative Dentistry; 歯質接着材料; 接着性修復

The Confocal Laser Scanning Microscope (CLSM) was used in this study as nondestructive testing method.Standardized cavities were prepared of extracted human teeth. The prepared specimen was restored with various adhesive systems experimentally. The restored specimens supplied to the dye penetration test with Rohdamine B solution.Microleakage of the sectioned specimens were evaluated with the CLSM.Conventionally, many researchers used technique of the dye penetration test that a microscope observed by pigment penetration and cutting of the later specimen for marginal sealing property of adhesive restoration.However it was possible to examine it in a non destruction state by CLSM and the use of fluorescence pigment in this study.Furthermore, examination was done which part a microleakage penetrate from the cavity margin. These results were reported in the Journal of Dental Research,80,81,82-Special Issue and the China-Japan Medical Conference 2002.

采用激光共聚焦扫描显微镜（CLSM）对离体牙进行标准化洞型的无损检测。对制备好的试件进行了不同粘接体系的粘接修复实验。修复后的标本用罗丹明B溶液进行染料渗透试验，用CLSM评价切片标本的微渗漏。许多研究者采用显微镜观察色素渗透和切割后标本的染料渗透试验技术来检测粘接修复体的边缘封闭性，但CLSM和此外，还检查了微渗漏从腔缘穿透的部位。这些结果发表在《牙科研究杂志》，80，81，82-特刊和2002年中日医学会议上。

项目成果

T.MASEKI: "SEM Observation of the Resin-Dentin Interface formed by Various One-Step Adhesive Systems"China-Japan Medical Conference 2002. Stomatology Abstract,47. (2002)

T.MASEKI：“各种一步粘合系统形成的树脂-牙本质界面的SEM观察”中日医学会议2002年。口腔医学摘要，47。

T.MASEKI: "SEM Observation of the Resin-Dentin Interface formed by Adhesive Systems"Journal of Dental Research. 80-special issue. 660 (2001)

T.MASEKI：“粘合剂系统形成的树脂-牙本质界面的扫描电镜观察”牙科研究杂志。

I.KIZUKI: "Microleakage of One-step and Two-step Adhesive systems under Thermocycling and Simultaneous Repeated Load"Journal of Dental Research. 80-Special Issue. 556 (2001)

I.KIZUKI：“热循环和同时重复负载下一步和两步粘合剂系统的微泄漏”牙科研究杂志。

T.MASEKI: "SEM Observation of the Resin-Dentin Interface formed by All-in one Adhesive Systems"Journal of Dental Research. 82-special issue. 599 (2003)

T.MASEKI：“多合一粘合剂系统形成的树脂-牙本质界面的扫描电镜观察”牙科研究杂志。

柵木寿男: "CLSMを用いたレジン-象牙質接合界面観察"接着歯学. 19巻1号. 7-15 (2001)

Toshio Zaki：“使用 CLSM 观察树脂-牙本质粘合界面”《粘合剂牙科》，第 19 卷，第 1. 7-15 期（2001 年）。