Microbicidal efficacy ozonated water against Candida albicans adhered to acrylic denture plate

众所周知，臭氧是一种很强的抗细菌、真菌和病毒的抗菌剂。我们研究了臭氧化水对人体的影响。丙烯酸义齿板上的白色念珠菌。[方法]用白色念珠菌培养热固化丙烯酸树脂。用无菌生理盐水洗涤后，在臭氧水（4mg /L）中浸泡60分钟，计数附着白色念珠菌的数量。在一些实验中，试验样品采用臭氧化水与超声波联合处理。<结果>臭氧水（2或4 mg/L）作用1分钟后，白色念珠菌细胞几乎不存在。臭氧水和超声波联合作用对粘附在丙烯酸树脂板上的白色念珠菌的生存能力有较强的影响。超声浸泡臭氧水与市售义齿清洗剂处理的钢板对白色念珠菌的抑菌活性无显著差异。此外，电镜分析显示，暴露于流动的臭氧化水或超声浸泡在臭氧化水后，在板上仍有少量白色念珠菌。结论臭氧水的应用可能有助于减少义齿板上白色念珠菌的数量。

<Objectives> Ozone is known to act as a strong antimicrobial agent against bacteria, fungi, and viruses. We examined the effect of ozonated water against. Candida albicans on acrylic denture plate. <Methods> The heat-cured acrylic resins were cultured with C. albicans. Following washing with sterile saline and immersion in ozonated water (4 mg/L) for 60 minutes, numbers of the attached C.albicans was counted. In some experiments, the test samples were treated with ozonated water in combination with ultrasonication. <Results> After exposure to flowing ozonated water (2 or 4 mg/L) for 1 minute, viable C.albicans cells were nearly nonexistent. The combination of ozonated water and ultrasonication had a strong effect on the viability of C.albicans adhered to the acrylic resin plates. There were no significant differences in antimicrobial activity against C.albicans between plates immersed in ozonated water with ultrasonication and those treated with commercially available denture cleaners. In addition, electron microscopic analysis revealed that small amounts of C.albicans were remained on the plate, after exposure to flowing ozonated water or immersion in ozonated water with ultrasonication. <Conclusion> Our results suggested that application of ozonated water may be useful for reducing the number of C.albicans on the denture plates.