Microbicidal efficacy ozonated water against Candida albicans adhered to acrylic denture plate

&lt；目标&gt；臭氧被认为是一种对细菌、真菌和病毒具有很强抗菌力的物质。我们考察了臭氧化水对环境的影响。丙烯酸义齿托板上的白色念珠菌。方法将热固化的丙烯酸树脂与白色念珠菌混合培养。用无菌生理盐水清洗，以4 mg/L的臭氧水浸泡60min后，计数附着的白色念珠菌数量。在一些实验中，测试样品被臭氧和超声波联合处理。结果流动臭氧水(2或4 mg/L)作用1min后，白色念珠菌几乎不存在。臭氧水和超声波的组合对附着在丙烯酸树脂平板上的白色念珠菌的活性有很大的影响。用超声臭氧浸泡的平板和用市售义齿清洁剂处理的平板对白色念珠菌的抗菌活性没有显著差异。此外，电子显微镜分析显示，暴露在流动的臭氧水中或通过超声波浸泡在臭氧水中后，平板上仍有少量白色念珠菌。结论我们的研究结果提示，应用臭氧水可能有助于减少义齿托板上的白色念珠菌数量。

<Objectives> Ozone is known to act as a strong antimicrobial agent against bacteria, fungi, and viruses. We examined the effect of ozonated water against. Candida albicans on acrylic denture plate. <Methods> The heat-cured acrylic resins were cultured with C. albicans. Following washing with sterile saline and immersion in ozonated water (4 mg/L) for 60 minutes, numbers of the attached C.albicans was counted. In some experiments, the test samples were treated with ozonated water in combination with ultrasonication. <Results> After exposure to flowing ozonated water (2 or 4 mg/L) for 1 minute, viable C.albicans cells were nearly nonexistent. The combination of ozonated water and ultrasonication had a strong effect on the viability of C.albicans adhered to the acrylic resin plates. There were no significant differences in antimicrobial activity against C.albicans between plates immersed in ozonated water with ultrasonication and those treated with commercially available denture cleaners. In addition, electron microscopic analysis revealed that small amounts of C.albicans were remained on the plate, after exposure to flowing ozonated water or immersion in ozonated water with ultrasonication. <Conclusion> Our results suggested that application of ozonated water may be useful for reducing the number of C.albicans on the denture plates.