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Study on pathogenesis of invebeterate oral mucous membrane diseases : Investigation of superoxide anion and various cytokine.

无病性口腔粘膜疾病发病机制的研究：超氧阴离子和各种细胞因子的研究。

基本信息

批准号：
13672092
负责人：
NARIAI Yoshiki
金额：
$ 1.79万
依托单位：
Shimane Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

The purpose of this study was to investigate the pathogenesis of various oral membrane diseases, in particular oral lichen planus, by examining the relationships between effects of biscoclaurine alkariods (BA; cepharanthin^<(R)>) on clinical symptoms and imunohistichemical stainings of cytokines or antioxidant enzymes. 30 tissue samples were obtained by biopsy from the patients with oral lichen planus. Immunostaining was performed using the streptavidin-biotin method. Correlation between Clinical improvement by treatment of biscoclaurine alkaloids and expressions of cytokines (TNF-α, IFN-γ, IL-1β) or antioxidant enzymes (manganese-containing superoxide dismutase : Mn-SOD, glutathione peroxidase : GPX) were evaluated using x^2-test and logistic regression. The results were as follow.1. The expressions of TNF-α, IFN-γ and IL-1β were detected in 17/30 (56.7%), 221/30 (73.3%) and 13/30 (43.3%) of the cases.2. TNF-α expression had significant relationship between IFN-γ or IL-1β expression.3. The negativity of TNF a was statistically correlated with effects of BA (P=0.046).4. The expressions of Mn-SOD and GPX were detected in 16/30 (53.3%) and 17/30 (56.7%) of the cases.5. The expression of Mn-SOD was a significant factor for the effects of BA on clinical symptoms.These results suggest that some kinds of cytokines and antioxidant enzymes may be related to pathogenesis of OLP and action of BA on OLP.

本研究的目的是通过检查双高月桂碱生物碱（BA；cepharanthin^<(R)>）对临床症状的影响与细胞因子或抗氧化酶的免疫组织化学染色之间的关系，研究各种口腔膜疾病，特别是口腔扁平苔藓的发病机制。通过活检从口腔扁平苔藓患者中获取30个组织样本。使用链霉亲和素-生物素方法进行免疫染色。使用 x^2 检验和逻辑回归评估双高月桂碱生物碱治疗的临床改善与细胞因子（TNF-α、IFN-γ、IL-1β）或抗氧化酶（含锰超氧化物歧化酶：Mn-SOD、谷胱甘肽过氧化物酶：GPX）表达之间的相关性。结果如下： 1． TNF-α、IFN-γ、IL-1β表达率分别为17/30(56.7%)、221/30(73.3%)、13/30(43.3%)。 2． TNF-α的表达与IFN-γ或IL-1β的表达有显着相关性。 3． TNFα的阴性与BA的作用有统计学相关(P=0.046)。4． Mn-SOD和GPX表达分别为16/30(53.3%)和17/30(56.7%)。5． Mn-SOD的表达是BA影响临床症状的重要因素。这些结果提示某些细胞因子和抗氧化酶可能与OLP的发病机制以及BA对OLP的作用有关。