Effect of-mechanicalstress on osteoblast and osteoclast like cell

机械应力对成骨细胞和破骨细胞样细胞的影响

• 批准号: 13672160
• 负责人: NAKAO Noriko
• 金额: $ 2.18万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672160/
• 关键词: Centrifugal force; Pulsing electromagnetic fields; Human osteoblast-like cell; Osteoclast; Intracellular response; Prostaglandin; Making to lime; Resorption of the bone

The mechanical stress, which were both the gravity and the electromagnetic field, stimulated the ALPase activity in a dose. dependent manner. The results suggested that the mechanical stress induced the differentiation of the human osteoblast-like cell. Furthermore, the pulse electromagnetic held load inhibited ALPase activity in the cell culture before the cell confluent. It. was clear that the pulse electromagnetic held load effected the human osteoblast-like cell in the proliferation the period. F6-1, 25(OH)2D3 and 1-25(OH)2D3 also effected the stimulation of the human osteoblast-like cell proliferation by the gravity and the electromagnetic field. In general, the increase of ALPase activity and the production of BGP, which was non-collagen substrate protein, were related each other. But, the different production of BMP in the gravity and in the pulse electromagnetic field was recognized in this study. However, the gravity, which was the centrifugal force, did not effect both the RANKL-and TNF-α-induced osteoclastogenesis. These results suggested that the mechanical was useful for bone formation because it induced the osteoblast-formation but not the osteoclastogenesis.

机械应力（包括重力和电磁场）刺激一定剂量的 ALPase 活性。依赖方式。结果表明机械应力诱导人成骨细胞样细胞的分化。此外，脉冲电磁保持负载在细胞汇合之前抑制细胞培养物中的 ALPase 活性。它。明确了脉冲电磁保持负载对人成骨细胞样细胞增殖期间的影响。 F6-1、25(OH)2D3和1-25(OH)2D3还通过重力和电磁场刺激人成骨细胞样细胞增殖。一般来说，ALPase活性的增加和非胶原蛋白底物BGP的产生是相互关联的。但是，本研究认识到在重力和脉冲电磁场​​中 BMP 的不同产生。然而，重力（即离心力）并不影响 RANKL 和 TNF-α 诱导的破骨细胞生成。这些结果表明机械对于骨形成有用，因为它诱导成骨细胞形成，但不诱导破骨细胞生成。

项目成果

K.Togashi, H.Kitaura, K.Yonetsu, N.Yoshida, T.Nakamura: "Three dimensional cephalometry using helical CT : Evaluation of measurement error in 3-D cephalometry caused by head inclination"Angle Orthodontist. 72(6). 513-520 (2002)

K.Togashi、H.Kitaura、K.Yonetsu、N.Yoshida、T.Nakamura：“使用螺旋 CT 的三维头影测量：评估由头部倾斜引起的 3D 头影测量中的测量误差”Angle Orthodontist。

Kitaura, H., Tatamiya, M., Nakao, N., Yoshida, N., et al.: "Interleukin-4 directly inhibits tumor necrosis factor- α -mediated osteoclast formation in mouse bone marrow macrophages"Immunol.Lett.. 88(3). 193-198 (2003)

Kitaura, H.、Tatamiya, M.、Nakao, N.、Yoshida, N.等人：“Interleukin-4 直接抑制小鼠骨髓巨噬细胞中肿瘤坏死因子-α 介导的破骨细胞形成”Immunol.Lett.. 88（3）。193-198（2003）

Kitaura, H., Tatamiya, M., Nakao, N., Yoshida, N.et al.: "Interleukin-4 directly inhibits tumor necrosis factor-α-mediated osteoclast formation in mouse bone marrow macrophages"Immunol.Lett.. 88(3). 193-198 (2003)

Kitaura, H.、Tatamiya, M.、Nakao, N.、Yoshida, N.等人：“Interleukin-4 直接抑制小鼠骨髓巨噬细胞中肿瘤坏死因子 α 介导的破骨细胞形成”Immunol.Lett.. 88 (3)。193-198(2003)。

Kitaura, H., Nakao, N., Yoshida, N., Yamada, T.: "Induced sensitization to nickel in guinea pigs immunized with mycobacteria by injection of purified protein derivative (PPD) with nickel"Microbiologica. 26(1). 101-108 (2003)

Kitaura, H.、Nakao, N.、Yoshida, N.、Yamada, T.：“通过注射含镍的纯化蛋白衍生物 (PPD) 来诱导用分枝杆菌免疫的豚鼠对镍的过敏”微生物学。

Kitaura, H., Nagata, N., Fujimura, Y., Hotokezaka, H., Yoshida, N., Nakayama, K.: "Effect of IL-12 on TNF-a-mediated osteoclast formation in bone marrow cells : apoptosis mediated by Fas/Fas ligand interactionl"Journal of Immunology. 169(9). 4732-4738 (20

Kitaura, H.、Nagata, N.、Fujimura, Y.、Hotokezaka, H.、Yoshida, N.、Nakayama, K.：“IL-12 对骨髓细胞中 TNF-a 介导的破骨细胞形成的影响：细胞凋亡