Effect of-mechanicalstress on osteoblast and osteoclast like cell

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The mechanical stress, which were both the gravity and the electromagnetic field, stimulated the ALPase activity in a dose. dependent manner. The results suggested that the mechanical stress induced the differentiation of the human osteoblast-like cell. Furthermore, the pulse electromagnetic held load inhibited ALPase activity in the cell culture before the cell confluent. It. was clear that the pulse electromagnetic held load effected the human osteoblast-like cell in the proliferation the period. F6-1, 25(OH)2D3 and 1-25(OH)2D3 also effected the stimulation of the human osteoblast-like cell proliferation by the gravity and the electromagnetic field. In general, the increase of ALPase activity and the production of BGP, which was non-collagen substrate protein, were related each other. But, the different production of BMP in the gravity and in the pulse electromagnetic field was recognized in this study. However, the gravity, which was the centrifugal force, did not effect both the RANKL-and TNF-α-induced osteoclastogenesis. These results suggested that the mechanical was useful for bone formation because it induced the osteoblast-formation but not the osteoclastogenesis.