Effect of-mechanicalstress on osteoblast and osteoclast like cell

机械应力，即重力和电磁场，在一定程度上刺激了ALPase的活性。依赖的方式。结果表明，机械应力诱导了人成骨样细胞的分化。此外，脉冲电磁负载在细胞融合前抑制了细胞培养中的ALPase活性。它。可见脉冲电磁载荷在增殖过程中对人成骨样细胞有影响。F6-1、25(OH)2D3和1-25(OH)2D3也能在重力和电磁场作用下刺激人成骨样细胞增殖。总的来说，ALPase活性的升高与非胶原底物蛋白BGP的产生是相互关联的。但是，本研究发现在重力和脉冲电磁场作用下BMP的产生是不同的。而重力（即离心力）对rankl和TNF-α诱导的破骨细胞生成均无影响。这些结果表明，机械作用对骨形成是有用的，因为它诱导成骨细胞的形成，而不是破骨细胞的形成。

The mechanical stress, which were both the gravity and the electromagnetic field, stimulated the ALPase activity in a dose. dependent manner. The results suggested that the mechanical stress induced the differentiation of the human osteoblast-like cell. Furthermore, the pulse electromagnetic held load inhibited ALPase activity in the cell culture before the cell confluent. It. was clear that the pulse electromagnetic held load effected the human osteoblast-like cell in the proliferation the period. F6-1, 25(OH)2D3 and 1-25(OH)2D3 also effected the stimulation of the human osteoblast-like cell proliferation by the gravity and the electromagnetic field. In general, the increase of ALPase activity and the production of BGP, which was non-collagen substrate protein, were related each other. But, the different production of BMP in the gravity and in the pulse electromagnetic field was recognized in this study. However, the gravity, which was the centrifugal force, did not effect both the RANKL-and TNF-α-induced osteoclastogenesis. These results suggested that the mechanical was useful for bone formation because it induced the osteoblast-formation but not the osteoclastogenesis.