Screening for Natural Medicines against Type-C Hepatitis Using Human-Liver-Derived Cell Line

研究了作为民间治疗肝炎药物的天然药物对过氧化叔丁基对人肝源细胞（HepG2细胞）肝毒性的影响。用于治疗慢性肝炎的甘草酸显示出明显的剂量依赖性保护作用。在抗肝炎的天然药物中，保肝中药葛根、草中主要的皂苷类化合物槐二醇的保肝活性与甘草酸苷相当。姜黄中的姜黄素I ~ III、水飞蓟中的水飞蓟素、水飞蓟素、黄芩中的黄酮类化合物、绿茶中的儿茶素也有一定的保肝活性。得到了一些结构-肝保护活性的关系。虽然没食子儿茶素邻苯三酚部分降低了活性，但没食子儿茶素没食子酸酯部分显著提高了活性。虽然没食子酸本身没有肝保护作用，但没食子酸的甲基lester显示出有效的活性。

The effects of natural medicines which were used as a folk medicine for hepatitis on the hepatotoxicity of tert-butyl hydroperoxide to human-liver-derived cell line (HepG2 cells) were investigated. Glycyrrhizin which is used to treat chronic hepatitis showed significant dose-dependent protective effects. Among the natural medicines which were used as ant-hepatitis, sophoradiol that is the main sapogenol in Puerariae Flos and Abri Herba which are known to be hepatoprotective crude drugs showed the most potent hepatoprotective activity equal to glycyrrhizin. Curcumins I〜III from turmeric, silybin, silychristin from milk thistle, some flavonoids from Scuterallia root, catechins from green tea also showed moderate hepatoprotective activity. Some structure-hepatoprotective activity relationships were obtained. Although pyrogallol moiety of epigallocatechin reduced the activity, gallic acid ester moiety of epigallocatechine gallate enhanced the activity remarkably. Although gallic acid itself did not show any hepatoprotective activity, the methylester of gallic acid showed potent activity.