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REGULATORY MECHANISMS FOR THE DENSITY AND PROPERTY OF K^+CHANNELS

K^ 通道密度和特性的调节机制

基本信息

批准号：
13680871
负责人：
WEN-JIE Song
金额：
$ 2.18万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680871/
关键词：
POTASSIUM CHANNEL KV4.1 KV4.2 STRIATUM RAT DEVELOPMENT CHANNEL DENSITY CHQLINERGIC INTERNEURQN αsubunit βsubunit delayed rectifier

项目摘要

Channel density is a fundamental factor determining neuronal firing, and is primarily regulated during development through transcriptional and translational regulation. In adult rats, striatal cholinergic interneurons have a prominent A-type current and co-express Kv4.1 and Kv4.2 mRNAs. The contribution of Kv4.2 and Kv4.1 to the A-type current in cholinergic interneurons during development, however, is not known. Using patch-clamp recording and semi-quantitative single-cell RT-PCR techniques, we have examined the postnatal development of A-type current and the expression of Kv4.2 and Kv4.1, in rat striatal cholinergic interneurons. A-type current was detectable at birth and its amplitude was up-regulated with age, reaching a plateau at about three weeks after birth. Kv4.2 mRNA was detectable at birth and the expression level increased exponentially with age, reaching a plateau by three weeks postnatal. In contrast, Kv4.1 mRNA was not detectable during the first week after birth, and the expression level did not show a clear tendency with age. Taken together, our results suggest that Kv4.2 plays an essential role in producing the A-type current in striatal cholinergic interneurons during the entire course of postnatal development.

通道密度是决定神经元放电的基本因素，并且主要在发育期间通过转录和翻译调节来调节。在成年大鼠中，纹状体胆碱能中间神经元具有显著的A型电流，并共表达Kv4.1和Kv4.2 mRNA。然而，Kv4.2和Kv4.1在发育过程中对胆碱能中间神经元A型电流的贡献尚不清楚。应用膜片钳技术和半定量单细胞RT-PCR技术，研究了大鼠纹状体胆碱能中间神经元A型电流的生后发育过程及Kv4.2和Kv4.1的表达。A型电流在出生时就可检测到，其幅度随着年龄的增长而上调，在出生后约三周达到平台。Kv4.2 mRNA在出生时可检测到，表达水平随年龄呈指数增加，出生后3周达到平台期。相反，Kv4.1 mRNA在出生后的第一周内未检测到，并且表达水平没有显示出明显的年龄趋势。两者合计，我们的研究结果表明，Kv4.2在整个出生后发育过程中产生纹状体胆碱能中间神经元的A型电流中起着至关重要的作用。