Studies on the Molecular Mechanism in Life-cycle Development and Signal Trnnsduction of Aphanomyces cochlioides, a Cause of Spinach Root Rot

导致菠菜根腐病的旋状丝丝菌生命周期发育及信号传导的分子机制研究

• 批准号: 14206013
• 负责人: TAHARA Satoshi
• 金额: $ 28.04万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14206013/
• 关键词: Aphanomyces cochlioides; zoospore attractant; chemotaxis; phytopathogenic Oomycetes; host-specific attractant; signal transduction; receptor; life-cycle development; 卵菌類; G蛋白質共役型受容体; Aphanomyces cochlioides; 形態変化; 情報伝達物質; 宿主認識化合物; 拮抗性根面細菌

1.The zoospores of phytopathogenic Oomycete, Aphanomyces cochlioides show clear positive chemotaxis to the host-specific singanl substances. The reputed receptor for the host-specific attractant toward the zoospores of spinach root rot pathogen, A.cochlioides wsa surveyed by using a biotin conjugated azidoflavone as a probe and resulted in 40 and 70 kDa membrane proteins on electropholetic gel plates.2.The events caused by signal transduction regarding the life-cycle development of a Peronosporomycete (Oomycete) Aphanonyres cochlioides has been studied by using microscopic techniques. An interesting difference in F-actin profiles was observe between two kinds of custospores, one developing ahead to hyphal germination and the other rejuvenating to regeneration of zoospores.3.About 150 rhizoplane bacteria were isolated from host and non-host plants, and our dual culture bioassay revealed that more than 5 % of isolates caused severe morphological alterations on phytopathogenic Oomycete Aphanomyces cochlioides and the fungal growth was strongly inhibited. From these results, we proposed that this method is new and effective way to find biological control agents against soilbome phytopathogens.4.Cochliophilin A (5-hydroxy-6,7-methylenedioxyflavone) known as the host-specific zoospore attractant for a spinach root rot pathogen has been isolated so far only from Chenopodiaceae plants, spinach, sugar beet, and Chenopodium album, however, in our recent survey of zoospore attractants in Amamthaceae plant, Celosia cristata revealed that one of the active princilpes was indeed cochliophilin A.

1.植物病原卵菌螺旋丝囊霉游动孢子对寄主特异性单体物质具有明显的正趋化性。利用生物素标记的叠氮双黄酮作为探针，对菠菜根腐病菌游动孢子的寄主特异性引诱剂受体进行了研究，并在电泳凝胶板上得到了分子量为40和70 kDa的膜蛋白。2.利用显微技术研究了霜霉纲（卵菌纲）螺旋隐甲藻（Aphanonyres cochlioides）生活史发育中的信号转导事件。两种保管孢子的F-肌动蛋白谱存在明显的差异，一种保存孢子发育到菌丝萌发之前，另一种保存孢子发育到游动孢子再生之前。3.从寄主和非寄主植物中分离到约150个根面细菌，我们的双重培养生物测定显示，的菌株对植物病原卵菌螺旋状丝壳霉的形态产生了严重的影响，真菌的生长受到强烈的抑制。因此，我们认为该方法是寻找土壤植物病原菌生防菌的一条新的有效途径（5-羟基-6，7-亚甲二氧基黄酮）已知为菠菜根腐病病原体的宿主特异性游动孢子引诱剂，迄今为止仅从藜科植物、菠菜、甜菜和藜中分离，然而，在我们最近对鹅膏蕈科植物鸡冠花游动孢子引诱剂的调查中，发现其中一种活跃的Princilpes确实是Cochliophilin A。

项目成果

Quantification of Particle Method for Chemotactic Bioassay Using Peronosporomycete Zoospores.

使用霜霉病游动孢子进行趋化生物测定的颗粒法定量。

• 发表时间: 2004
• 期刊: Z.Naturforsch. 59c
• 作者: T.Takayama;Y.Fukushi;Md.T.Islam;S.Tahara
• 通讯作者: S.Tahara

Hiroshi Ishimoto, Yukiharu Fukushi, Satoshi Tahara: "Non-pathogenic Fusarium strains protect the seedlings of Lepidium sativum from Pythium ultimum"Soil Biology and Biochemistry. (in press). (2004)

Hiroshi Ishimoto、Yukiharu Fukushi、Satoshi Tahara：“非致病性镰刀菌菌株保护 Lepidium sativum 幼苗免受终极腐霉侵害”土壤生物学和生物化学。

A Photoaffinity Probe Desighned for Host-Sprecific Signal Flavonoid Receptors in Phytopathogenic Peronosporomycete Aphanomyces cochlioides.

专为植物病原性霜霉病 Aphanomyces cochlioides 中宿主特异性信号黄酮类受体设计的光亲和探针。

• 发表时间: 2004
• 期刊: Arch.Biophys.Biochem. 432
• 作者: Y.Sakihama;T.Shimai;M.Sakasai;T.Ito;Y.Fukushi;Y.Hashidoko;S.Tahara
• 通讯作者: S.Tahara

A Phtotoaffinity Probe Designed for Host-Specific Signal Flavonoid Receptors in Phytopathogenic Peronosporomycete Zoospores of Aphanomyces cochlioides.

专为植物病原性霜霉菌游动孢子中的寄主特异性信号类黄酮受体设计的光亲和探针。

• 发表时间: 2004
• 期刊: Arch.Biochem.Biophys. 432
• 作者: Yasuko Sakihama;Takashi Shimai;Mitsuyoshi Sakasai;Toshiaki Ito;Yukiharu Fukushi;Yasuyuki Hashidoko;Satoshi Tahara
• 通讯作者: Satoshi Tahara

Host-specific plant signal and G-protein activator, mastoparan, trigger differentiation of zoospores of the phytopathogenic oomycete Aphanomyces cochlioides

• DOI: 10.1023/a:1026114731718
• 发表时间: 2003-08-01
• 期刊: PLANT AND SOIL
• 影响因子: 4.9
• 作者: Islam, MT;Ito, T;Tahara, S
• 通讯作者: Tahara, S