Electron transfer and early evolution of photosynthesis in newly isolated green filamentous bacteria

新分离的绿色丝状细菌的电子转移和光合作用的早期进化

• 批准号: 14540597
• 负责人: MATSUURA Katsumi
• 金额: $ 2.37万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540597/
• 关键词: Photosynthetic bacteria; Purple bacteria; Green bacteria; Electron transfer; Photosynthetic genes; Cytochrome; 銅タンパク質; 緑色糸状細菌; 光合成反応中心; オーラシアニン

The photochemical reaction center (RC) complex of Roseiflexus (R) castenholzii, which belongs to filamentous anoxygenic phototrophic bacteria (green filamentous bacteria) but lacks chlorosomes was isolated and characterized. The genes coding for the subunits of RC and the light-harvesting proteins were also cloned and sequenced. The RC complex was composed of L, M and cytochrome subunits. The cytochrome subunit showed a molecular mass of approximately 35 kDa, contained hemes c, and worked as the electron donor to the photooxidized special pair of bacteriochlorophyll (BChl) in the RC. The RC complex appeared to contain three molecules of BChl and three molecules of bacteriopheophytin (BPhe) as in the RC preparation from chloroflexus (Cfl) aurantiacus. The phylogenetic trees based on the deduced amino acid sequences of the RC subunits suggested that R.castenholzii had diverged from Cfl.aurantiacus very early after the divergence of filamentous anoxygenic phototrophic bacteria from purple bacteria. Although R.castenholzii is phylogenetically related to Cfl.aurantiacus, the arrangement of its puf genes, which code for the light-harvesting and the RC subunits, was different from that of Cfl.aurantiacus and similar to those in purple bacteria in the order of puf B, -A, -L, -M, and -C. Since the photosynthetic apparatus and genes of R.castenholzii have intermediary characteristics between purple bacteria and Cfl.aurantiacus, it is likely that they retain many features of the common ancestor of purple bacteria and filamentous anoxygenic phototrophic bacteria.

分离并鉴定了属于丝状厌氧光营养细菌(绿丝状细菌)但缺乏叶绿体的Roseifleus(R)Castenholzii光化学反应中心(RC)复合体。编码RC亚基和捕光蛋白的基因也被克隆和测序。RC复合体由L亚基、M亚基和细胞色素亚基组成。细胞色素亚基分子量约为35 kDa，含有氯化血红素c，是光氧化后的特殊对细菌叶绿素(BCHL)的电子供体。该RC复合体似乎含有3个BCHL分子和3个细菌素(BPhe)分子，与从白屈菜(CFL)提取的RC制剂一样。根据推测的RC亚基氨基酸序列构建的系统发育树表明，在丝状厌氧光营养细菌从紫色细菌分化之后，卡森霍尔兹氏杆菌很早就从橙色丝状细菌分化而来。虽然卡森霍尔兹氏杆菌与橙色假单胞菌有亲缘关系，但其编码捕光和RC亚基的PUF基因的排列与橙色假单胞菌不同，与紫色细菌的排列顺序为PUF B、-A、-L、-M和-C。由于R.Castenholzii的光合作用装置和基因具有介于紫色细菌和Cfl.aurantiacus之间的中间特征，因此它们可能保留了紫色细菌和丝状厌氧光营养细菌的共同祖先的许多特征。

项目成果

Tsukatani, Y.: "Phylogenetic distribution of unusual triheme to teteraheme Cytochorome subunit in the reaction center complex of purple bacteria."Photosynthesis Research. 79. 83-91 (2004)

Tsukatani, Y.：“紫色细菌反应中心复合体中不寻常三血红素到四血红素细胞色素亚基的系统发育分布。”光合作用研究。

Hanada, S.et al.: "Roseiflexus castenholzii gen.nov., sp nov.a themophilic, filamentous, photosynthetic bacterium whih lack chlorosomes"Intemational Joumal of Systematic and Evolutionary Microbiology. 52. 187-193 (2002)

Hanada, S.等人：“Roseiflexus Castenholzii gen.nov.，sp nov.a 缺乏叶绿体的嗜热丝状光合细菌”国际系统与进化微生物学杂志。

Tsukatani, Y.et al.: "Phylogenetic distribution of unusual triheme cytochrome subunit in the reaction center complex of purple bacteria."Photosynthesis Research. 79. 83-91 (2004)

Tsukatani, Y.等人：“紫色细菌反应中心复合体中不寻常三血红素细胞色素亚基的系统发育分布。”光合作用研究。

Tsukatani, Y.: "Phylogenetic distribution of unusual triheme to teteraheme Cytochorome subunit in the reaction center complex of purple bacgteria."Photosynthesis Research. 79. 83-91 (2004)

Tsukatani, Y.：“紫色细菌反应中心复合体中不寻常三血红素到四血红素细胞色素亚基的系统发育分布。”光合作用研究。

Hanada, S: "Roseiflexus castenholzii gen.nov.,sp.nov.,a thermophilic, filamentous, photosynthetic bacterium which lacks chlorosomes"International Journal of Systematic and Evolutionary Microbiology. 52. 187-193 (2002)

Hanada, S：“Roseiflexus Castenholzii gen.nov.，sp.nov.，一种缺乏叶绿体的嗜热丝状光合细菌”《国际系统与进化微生物学杂志》。