Development Of Chemiluminometric Flow-Through Sensor For Simultaneous Determination of Neurotransmitters

开发用于同时测定神经递质的化学发光流通式传感器

• 批准号: 14550780
• 负责人: KIBA Nobutoshi
• 金额: $ 2.3万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14550780/
• 关键词: SIMULTANEOUS ASSAY; FIA; CHEMILUMINOMETRIC; FLOW-THROUGH SENSOR; IMMOBILIZED ENZYMES; HIGH THROUGHPUT; BIOMATERIALS; NEUROTRANSMITTERS; 化学発光; フローセンサー; フロー法; 小型化; バイオセンサ

Three kinds of chemiluminometric flow-through sensors for simultaneous determination of organic compounds in a single sample have been developed. First, for simultaneous determination of urate, L-glutamate, and L-lysine in serum, immobilized uricase (EC1.7.3.3), immobilized peroxidase (EC1.11.1.7), support material, coimmobilized L-glutamate oxidase (EC1.4.3.11)/peroxidase, support material, and coimmobilozed L-lysine oxidase (EC1.4.3.14)/peroxidase were packed sequentially in a transparent FEP tube (1mm i.d, 75 cm length), and the tube was placedin front of a photomultiplier tube as a flow cell. A three-peak recording was obtained by one injection of the sample solution. The analytes were determined from 50 to 1000 nM, respectively, and sampling rate was 11/h without carryover. The detection limits were 5 nM urate, 20 nM L-glutamte, and 40 nM L-lysine. Next, for simultaneous determination of chorine and acetylcholine in brain tissue homogenates, immobilized chorine oxidase(EC1.1.3.7), immobilized peroxidase, immobilized acetylcholine esterase(EC3.1.1.7), and coimmobilized chorine oxidase/peroxidase were packed, in turn, in the FEP tube and used as a flow cell. The analytes were determined from 3 to 1000 nM, respectively, and the sample throughput was 25/h. The detection limit were 0.5 nM chorine and 2 nM acetylcholine. Finally, for simultaneous determination of D-glucose and 3-hydroxybutyrate in serum, coimmobilized 3-hydroxybutyrate dehydrogenase/NADH oxidase/peroxidase, a support material, and coimmobilized glucose dehydrogenase/NADH oxidase/peroxidase were packed, in turn, in the FEP tube and used as a flow cell. The analytes were determined from 0.1 to 30 μM, respectively, and sample throughput was 30/h. The detection limit were 10nM 3-hydorxybutyrate and 50 nM D-glucose.

研制了三种用于同时测定单一样品中有机化合物的化学发光通流传感器。首先，为了同时测定血清中的尿酸、l -谷氨酸和l -赖氨酸，将固定化尿酸酶（EC1.7.3.3）、固定化过氧化物酶（EC1.11.1.7）、支撑材料、共固定化l -谷氨酸氧化酶(EC1.4.3.11)/过氧化物酶、支撑材料和共固定化l -赖氨酸氧化酶(EC1.4.3.14)/过氧化物酶依次装在透明FEP管（直径1mm，长度75cm）中，将管置于光电倍增管前作为流动池。一次注射样品溶液，得到三峰记录。分析物分别在50 ~ 1000 nM范围内测定，采样率为11/h，无残留。检出限分别为精氨酸5 nM、谷氨酸20 nM、赖氨酸40 nM。接下来，为了同时测定脑组织匀浆中的氯和乙酰胆碱，依次将固定化的氯氧化酶（EC1.1.3.7）、固定化的过氧化物酶、固定化的乙酰胆碱酯酶（EC3.1.1.7）和共固定化的氯氧化酶/过氧化物酶包装在FEP管中作为流动细胞。分析物测定范围为3 ~ 1000 nM，样品通量为25/h。检出限分别为0.5 nM氯和2 nM乙酰胆碱。最后，为了同时测定血清中的d -葡萄糖和3-羟基丁酸盐，将共固定的3-羟基丁酸脱氢酶/NADH氧化酶/过氧化物酶作为载体材料，依次装入FEP管中作为流动细胞。测定范围为0.1 ~ 30 μM，样品通量为30/h。检出限为3-羟基丁酸盐10nM， d -葡萄糖50nm。

项目成果

Retention behavior of monosaccharides and disaccharides on titania

• DOI: 10.1007/bf02492416
• 发表时间: 2003-03
• 期刊: Chromatographia
• 影响因子: 1.7
• 作者: K. Tani;M. Kitada;M. Tachibana;H. Koizumi;T. Kiba

Ariumi Kawamoto: "Regeneration and Reuse of Hydrotalcite-Like Anion Exchanger with High Selectivity to Phosphate Anion."Journal of the Society of Inorganic Materials, Japan. 10. 167-172 (2003)

Ariumi Kawamoto：“对磷酸根阴离子具有高选择性的类水滑石阴离子交换剂的再生和再利用。”日本无机材料学会杂志。

REGENERATION AND REUSE OF HYDROTALCITE-LIKE ANION EXCHANGER WITH HIGH SELECTIVITY TO PHOSHATE ANION.

对磷酸根阴离子具有高选择性的类水滑石阴离子交换剂的再生和再利用。

• 发表时间: 2003
• 期刊: JOURNAL OF THE SOCIETY OF INORGANIC MATERIALS, JAPAN 10
• 作者: Ariumi Kawamoto;Nobutoshi Kiba;Kazue Tani;Nobutoshi Kiba;加藤智和;Nobutoshi Kiba;ARIUMI KAWAMOTO
• 通讯作者: ARIUMI KAWAMOTO

Nobutoshi Kib: "Chemiluminometric Sensor for Simultaneous Determination of L-Glutamate and L-Lysine with Immobilized Oxidases in a Flow…"Analytical Chemistry. 74. 1269-1274 (2002)

Nobutoshi Kib：“用固定化氧化酶在流动中同时测定 L-谷氨酸和 L-赖氨酸的化学发光传感器……”分析化学。74。1269-1274 (2002)

Nobutoshi Kiba: "Flow-through Micro Sensor Using Immobilized Peroxidase with Chemiluminometric FIA System for Determining Hydrogen Peroxide."Analytical Sciences. 19. 823-827 (2003)

Nobutoshi Kiba：“流通式微传感器使用固定化过氧化物酶和化学发光 FIA 系统来测定过氧化氢。”分析科学。