Development Of Chemiluminometric Flow-Through Sensor For Simultaneous Determination of Neurotransmitters

开发用于同时测定神经递质的化学发光流通式传感器

• 批准号: 14550780
• 负责人: KIBA Nobutoshi
• 金额: $ 2.3万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14550780/
• 关键词: SIMULTANEOUS ASSAY; FIA; CHEMILUMINOMETRIC; FLOW-THROUGH SENSOR; IMMOBILIZED ENZYMES; HIGH THROUGHPUT; BIOMATERIALS; NEUROTRANSMITTERS; 化学発光; フローセンサー; フロー法; 小型化; バイオセンサ

Three kinds of chemiluminometric flow-through sensors for simultaneous determination of organic compounds in a single sample have been developed. First, for simultaneous determination of urate, L-glutamate, and L-lysine in serum, immobilized uricase (EC1.7.3.3), immobilized peroxidase (EC1.11.1.7), support material, coimmobilized L-glutamate oxidase (EC1.4.3.11)/peroxidase, support material, and coimmobilozed L-lysine oxidase (EC1.4.3.14)/peroxidase were packed sequentially in a transparent FEP tube (1mm i.d, 75 cm length), and the tube was placedin front of a photomultiplier tube as a flow cell. A three-peak recording was obtained by one injection of the sample solution. The analytes were determined from 50 to 1000 nM, respectively, and sampling rate was 11/h without carryover. The detection limits were 5 nM urate, 20 nM L-glutamte, and 40 nM L-lysine. Next, for simultaneous determination of chorine and acetylcholine in brain tissue homogenates, immobilized chorine oxidase(EC1.1.3.7), immobilized peroxidase, immobilized acetylcholine esterase(EC3.1.1.7), and coimmobilized chorine oxidase/peroxidase were packed, in turn, in the FEP tube and used as a flow cell. The analytes were determined from 3 to 1000 nM, respectively, and the sample throughput was 25/h. The detection limit were 0.5 nM chorine and 2 nM acetylcholine. Finally, for simultaneous determination of D-glucose and 3-hydroxybutyrate in serum, coimmobilized 3-hydroxybutyrate dehydrogenase/NADH oxidase/peroxidase, a support material, and coimmobilized glucose dehydrogenase/NADH oxidase/peroxidase were packed, in turn, in the FEP tube and used as a flow cell. The analytes were determined from 0.1 to 30 μM, respectively, and sample throughput was 30/h. The detection limit were 10nM 3-hydorxybutyrate and 50 nM D-glucose.

已经开发了三种用于同时测定单个样品中有机化合物的化学流通传感器。 First, for simultaneous determination of urate, L-glutamate, and L-lysine in serum, immobilized uricase (EC1.7.3.3), immobilized peroxidase (EC1.11.1.7), support material, coimmobilized L-glutamate oxidase (EC1.4.3.11)/peroxidase, support material, and coimmobilized L-lysine oxidase （EC1.4.3.14）/过氧化物酶依次堆积在透明的FEP管（1mm I.D，75 cm的长度）中，将管放在光电倍增管的前面，作为流动池。通过一次注入样品溶液获得了三峰记录。分析物分别从50至1000 nm确定，采样率为11/h，无关。检测限为5 nm尿酸盐，20 nm l-谷氨酸和40 nm l-赖氨酸。接下来，为了简单地确定脑组织匀浆中的欺骗和乙酰胆碱，固定的欺骗氧化酶（EC1.1.3.7），固定的过氧化物酶，固定的乙酰胆碱酯酶（EC3.1.1.7），并在TORPASE中使用了Flof cell cell and toep and toep and toep and toep and toep and toep cell and toep and toep，分析物分别从3至1000 nm确定，样品吞吐量为25/h。检测极限为0.5 nm阳离子和2 nm乙酰胆碱。最后，为了简单地测定血清中D-葡萄糖和3-羟基丁酸，共摄取的3-羟基丁酸甲酸二酸酯脱氢酶/NADH氧化酶/过氧化物酶，一种支持材料，辅助型葡萄糖脱氢酶/NADHHOXIDASE/NADHOXIDASE/PEREXID CYLASE和PERNUBE均用Aep and theep and theep and the flof。分析物分别从0.1到30μm确定，样品吞吐量为30/h。检测极限为10nm 3-羟基丁酸和50 nm D-葡萄糖。

项目成果

Ariumi Kawamoto: "Regeneration and Reuse of Hydrotalcite-Like Anion Exchanger with High Selectivity to Phosphate Anion."Journal of the Society of Inorganic Materials, Japan. 10. 167-172 (2003)

Ariumi Kawamoto：“对磷酸根阴离子具有高选择性的类水滑石阴离子交换剂的再生和再利用。”日本无机材料学会杂志。

Nobutoshi Kiba: "Flow-through Micro Sensor Using Immobilized Peroxidase with Chemiluminometric FIA System for Determining Hydrogen Peroxide."Analytical Sciences. 19. 823-827 (2003)

Nobutoshi Kiba：“流通式微传感器使用固定化过氧化物酶和化学发光 FIA 系统来测定过氧化氢。”分析科学。

REGENERATION AND REUSE OF HYDROTALCITE-LIKE ANION EXCHANGER WITH HIGH SELECTIVITY TO PHOSHATE ANION.

对磷酸根阴离子具有高选择性的类水滑石阴离子交换剂的再生和再利用。

• 发表时间: 2003
• 期刊: JOURNAL OF THE SOCIETY OF INORGANIC MATERIALS, JAPAN 10
• 作者: Ariumi Kawamoto;Nobutoshi Kiba;Kazue Tani;Nobutoshi Kiba;加藤智和;Nobutoshi Kiba;ARIUMI KAWAMOTO
• 通讯作者: ARIUMI KAWAMOTO

Nobutoshi Kib: "Chemiluminometric Sensor for Simultaneous Determination of L-Glutamate and L-Lysine with Immobilized Oxidases in a Flow…"Analytical Chemistry. 74. 1269-1274 (2002)

Nobutoshi Kib：“用固定化氧化酶在流动中同时测定 L-谷氨酸和 L-赖氨酸的化学发光传感器……”分析化学。74。1269-1274 (2002)

Nobutoshi Kiba: "Chemiluminometric Sensor for Simultaneous Determination of L-Glutamate and L-Lysine with Immobilized Oxidases in a Flow Injection System."Analytical Chemistry. 74. 1269-1274 (2002)

Nobutoshi Kiba：“用于在流动注射系统中用固定化氧化酶同时测定 L-谷氨酸和 L-赖氨酸的化学发光传感器。”分析化学。