Organisation, expression and diversity of the sub-telomeric regions of the ancient eukaryote, Giardia duodenalis

古代真核生物十二指肠贾第鞭毛虫亚端粒区域的组织、表达和多样性

• 批准号: DP0346797
• 负责人: Dr Peter Upcroft
• 金额: $ 17.8万
• 依托单位: Queensland Institute of Medical Research
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0346797
• 关键词: Organisation; expression; diversity; sub; telomeric

We propose to extend our findings on the extreme plasticity of the structure and organisation of the sub-telomeric region of the complete genome of Giardia by more extensive chromosome walking, and comparison of different isolates. These regions are subject to gene conversion, transcriptional silencing, gene mobility, recombination, variable surface protein expression, subtelomeric instability and the insertion of transposable elements, a dynamic balance between structural conservation and rapid evolution. This is a rare opportunity to understand the forces at work in moulding eukaryotic sub-telomeric sequences because Giardia is not constrained by sexual homogenisation and the dynamic variability is retained.

我们建议通过更广泛的染色体行走和不同分离株的比较来扩展我们关于贾第虫全基因组亚端粒区域结构和组织的极端可塑性的发现。这些区域受到基因转换、转录沉默、基因迁移率、重组、可变表面蛋白表达、亚端粒不稳定性和转座元件插入的影响，结构保守和快速进化之间的动态平衡。这是一个难得的机会来了解在塑造真核亚端粒序列中起作用的力量，因为贾第虫不受性别同质化的限制，并且保留了动态可变性。