Isokinetic Closed Kinetic Chain Exercise using Electric Muscle Stimulation

使用电肌肉刺激的等速闭合动力链运动

• 批准号: 15300208
• 负责人: KAWAMURA Kenji
• 金额: $ 7.49万
• 依托单位: KIBI International University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15300208/
• 关键词: electrical stimulation; closed kinetic chain; muscle contraction; muscle training; neural cell culture; neurite outgrowth; rehabilitation; PC12 cell

1.Isokinetic closed kinetic chain exercise using electric muscle stimulationA new isokinetic testing machine was developed for CKC exercises. With this machine, subjects can move their legs in gradual arc of motions. A custom made foot plate and load cell were attached to the tip of dynamometer arm. When rectus femoris muscle was stimulated by the electric muscle stimulator, the output was displaced in a tiptoe direction. When vastus medialis and vastus lateralis were stimulated by the electric muscle stimulator, the output direction did not change. When hamstring muscle was stimulated by the electric muscle stimulator, the output was displaced in a calx direction. It is speculated that bi-articular muscle contributes to control of an output direction. On the other hand, mono-articular muscle do not contribute to control of an output direction. It is speculated that mono-articular muscle contributes to an absolute value of output force.2.Electrical stimulation induces neurite outgrowth in PC12m3 cells via the p38 MAP kinase pathwayWe developed a neural cell line PC12m3 which show neurite outgrowth by various physical stimuli. When cultures of the PC12 mutant (PC12m3) cells were exposed to electrical stimulation, activity of p38 MAP kinase increased and neurite outgrowth was greatly enhanced. The neurite extension was inhibited by the p38 MAP kinase inhibitor BS203580. Longer electrical stimulation of PC12m3 cells provoked cell death, which was enhanced by SB203580. Furthermore, we detected activation of cyclic AMP responsive element binding protein (CREB) by electrical stimulation in PC12m3 cells. CREB is a transcription factor that is the target of ERK. These findings suggest that electrical stimulation induce neurite outgrowth through p38 MAP kinase and CREB pathways in PC12m3 cells and that p38 MAP kinase is responsible for the survival of neuronal cells.

1.研制了一种新型的肌电刺激闭式运动链等速运动试验机。有了这个机器，受试者可以以逐渐的弧线运动来移动他们的腿。一个定制的脚板和称重传感器连接到测力器臂的尖端。当电肌刺激器刺激股直肌时，输出向脚尖方向移位。当电肌刺激器刺激股内侧肌和股外侧肌时，输出方向没有改变。当肌电刺激器刺激腘绳肌时，肌输出向轴向移位。推测双关节肌参与了输出方向的控制。另一方面，单关节肌不参与输出方向的控制。据推测，单关节肌对输出力的绝对值有贡献。电刺激通过p38 MAP激酶途径诱导PC12m3细胞的神经突生长我们培育了一株神经细胞系PC12m3，它在各种物理刺激下显示出神经突生长。当PC12突变体（PC12m3）细胞的培养暴露于电刺激时，p38 MAP激酶的活性增加，神经突起的生长大大增强。p38 MAP激酶抑制剂BS203580抑制了神经突的延伸。长时间电刺激PC12m3细胞可引起细胞死亡，SB203580可增强细胞死亡。此外，我们在PC12m3细胞中检测到电刺激激活环AMP响应元件结合蛋白（CREB）。CREB是一种转录因子，是ERK的靶标。这些发现表明，电刺激通过PC12m3细胞中的p38 MAP激酶和CREB途径诱导神经突生长，p38 MAP激酶与神经元细胞的存活有关。

项目成果

前十字靭帯損傷膝における開運動連鎖及び閉運動連鎖による下肢筋力評価

使用开放式和封闭式动力链评估前交叉韧带损伤膝关节下肢肌力

• 发表时间: 2004
• 期刊: 日本整形外科学会雑誌 78巻2号
• 作者: 阿部信寛;河村顕治 他
• 通讯作者: 河村顕治 他

足部がサイクロイド曲線を描く Isokinetic Closed Kinetic Chain Exercise における膝関節反カの筋骨格モデル解析

足部形成摆线的等速闭合运动链运动中膝关节受力的肌肉骨骼模型分析

• 发表时间: 2004
• 期刊: 日本整形外科学会雑誌 78巻 8号
• 作者: 松尾高行;河村顕治 他;河村顕治 他
• 通讯作者: 河村顕治 他

足部がサイクロイド曲線を描くIsokinetic Closed Kinetic Chain Exerciseにおける膝関節反力の筋骨格モデル解析

足部形成摆线的等速闭合动力链运动中膝关节反作用力的肌肉骨骼模型分析

• 发表时间: 2004
• 期刊: 日本整形外科学会雑誌 78巻8号
• 作者: 松尾高行;河村顕治 他;河村顕治 他
• 通讯作者: 河村顕治 他

Isokinetic Closed Kinetic Chain Exercises in Patients with ACL Deficient Knees

ACL 缺陷膝关节患者的等速闭合动力链练习

• 发表时间: 2005
• 期刊: International Society of Physical and Rehabilitation Medicine-ISPRM Proceedings of the 3rd World Congress
• 作者: K.KAWAMURA;et al.
• 通讯作者: et al.

Closed Kinetic Chain による下肢筋カ評価

使用闭合动力学链评估下肢肌肉力量

• 发表时间: 2005
• 期刊: 運動療法と物理療法 Vol.16 No.1
• 作者: K.KAWAMURA;et al.;河村顕治ほか
• 通讯作者: 河村顕治ほか