Functional analysis of genes for tyrosine catabolism that may be involved in annual and perennial differentiation of rice

可能参与水稻一年生和多年生分化的酪氨酸分解代谢基因的功能分析

• 批准号: 15380003
• 负责人: NAKAMURA Ikuo
• 金额: $ 10.18万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380003/
• 关键词: annual; perennial habitat; GSTZ gene; Oryza sativa; O. glaberrima; genomic walking; gene disruption; 遺伝子欠損; プロモーター; 遺伝子発現; 定量PCR法

Although glutathione S-transferases (GSTs) are family of enzyme involved in the detoxification of per-oxidized materials in cells, Zeta class of GST (GSTZ) is reported as the maleylacetoacetate isomerase (MAAI) which detoxify homogentisate, intermediate in the catabolic pathway of tyrosine and phenylalanine. The genome of rice contains two copies of gene encoding GSTZ. Two GSTZ genes of O. sativa, OsGSTZ1 and OsGSTZ2, lies in tandem-arranged orientation. Upstream OsGSTZ1 gene constitutively expresses whereas downstream OsGSTZ2 gene is inducible by stresses. We analyzed the expression of GSTZ gene in African cultivated species O. glaberrima and wild species O. longistaminata by using RT-PCR. The result showed that both GSTZ1 and GSTZ2 genes expressed in O. longistaminata, whereas only cDNA fragment of downstream GSTZ2 was detected in 0. glaberrima. Thus, the genomic sequence of GSTZ1 locus of O. glaberrima was determined by PCR genomic walking. The sequence comparison between O. sativa and O. glaberrima revealed that genomic sequence upstream from the eighth intron of OgGSTZ1 gene was highly homologous, in inverted orientation, to a BAC clone, apart over 1-Mb from OsGSTZ1 gene in O. sativa. This result suggested that OgGSTZ1 gene was disrupted by huge rearrangement or inversion. The genetic alteration was shared with several lines of O. glaberrima and its ancestral wild species O. barthii. The lost of OgGSTZ1 gene in O. glaberrima is interesting which is mainly expressed 12-fold higher than OsGSTZ2 in O. sativa and some different characters exist between O. sativa and O. glaberrima, like seed yield and annual/perennial habitat that might be related with the translocation of nitrogen metabolites from leaves to seeds. GSTZ1 gene is being introduced into O. glaberrima to convert annual to perennial habitat.

虽然谷胱甘肽 S-转移酶 (GST) 是参与细胞内过氧化物质解毒的酶家族，但 Zeta 类 GST (GSTZ) 被报道为马来酰乙酰乙酸异构酶 (MAAI)，它可以解毒尿黑酸，尿黑酸是酪氨酸和苯丙氨酸分解代谢途径中的中间体。水稻基因组含有两个编码 GSTZ 的基因拷贝。水稻的两个 GSTZ 基因 OsGSTZ1 和 OsGSTZ2 呈串联排列。上游 OsGSTZ1 基因组成型表达，而下游 OsGSTZ2 基因则受胁迫诱导。我们通过RT-PCR 分析了非洲栽培种O. glaberrima 和野生种O. longistaminata 中GSTZ 基因的表达。结果表明，GSTZ1和GSTZ2基因在长雄草中均表达，而在光果草中仅检测到下游GSTZ2的cDNA片段。因此，通过PCR基因组步移确定了O. glaberrima的GSTZ1基因座的基因组序列。水稻和光穗稻之间的序列比较表明，OgGSTZ1 基因第八内含子上游的基因组序列与 BAC 克隆高度同源，与 O. sativa 中的 OsGSTZ1 基因有超过 1 Mb 的同源性。这一结果表明OgGSTZ1基因因巨大的重排或倒位而被破坏。这种遗传改变与 O. glaberrima 及其祖先野生物种 O. barthii 的几个品系共有。 OgGSTZ1 基因在 O. glaberrima 中的丢失很有趣，该基因的表达量比 O. sativa 中的 OsGSTZ2 高 12 倍，并且 O. sativa 和 O. glaberrima 之间存在一些不同的特征，例如种子产量和一年生/多年生生境，这可能与氮代谢物从叶子到种子的易位有关。 GSTZ1 基因被引入 O. glaberrima 中，将一年生栖息地转变为多年生栖息地。

项目成果

Dual origin of the cultivated rice based on molecular markers of newly collected annual and perennial strains of wild rice species, Oryza nivara and O. rufipogon

• DOI: 10.1023/a:1023926802198
• 发表时间: 2003-02
• 期刊: Genetic Resources and Crop Evolution
• 影响因子: 2
• 作者: S. Yamanaka;I. Nakamura;H. Nakai;Yo-ichiro Sato

中村 郁郎: "アジア遊学(アンコール周辺の野生イネ)"勉誠出版. 175 (2003)

中村郁郎：《亚洲之旅（吴哥周围的野生稻）》 Bensei Publishing 175（2003）。

The sequences of the plastid gene rpl16 and the rpl16-rpl14 spacer region allow discrimination among six species of Scutellaria.

• DOI: 10.1016/j.jep.2004.02.031
• 发表时间: 2005-05
• 期刊: Journal of ethnopharmacology
• 影响因子: 5.4
• 作者: K. Hosokawa;M. Minami;I. Nakamura;A. Hishida;T. Shibata

Genomic structure and differential expression of two tandem-arranged GSTZ genes in rice.

• DOI: 10.1016/j.gene.2004.03.020
• 发表时间: 2004-06
• 期刊: Gene
• 影响因子: 3.5
• 作者: Tokuji Tsuchiya;T. Takesawa;H. Kanzaki;I. Nakamura

アジア遊学(アンコール周辺の野生イネ

亚洲之旅（吴哥周边野稻）

• 发表时间: 2003
• 作者: Ishiikawa;R,.I.Nakamura(他8名);中村 郁郎
• 通讯作者: 中村 郁郎