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Molecular analyses of ambrosia beetles and its symbiotic fungi associated with mass mortalities of oak

与橡树大量死亡相关的豚草甲虫及其共生真菌的分子分析

基本信息

批准号：
15380103
负责人：
ITO Shin-ichiro
金额：
$ 9.73万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380103/
关键词：
Raffaelea quercivora Platypus quercivorus mass mortality of oak trees area difference superficial morphology DNA analyses ITS region D1 D2 region ハンノキキクイムシ mtDNA RFLP解析 IGS領域ナラ類集団枯死被害リボソームDNA 5.8S-ITS2領域形態的特異性交配実験

项目摘要

To clarify the differences in morphological and molecular characteristics of a pathogenic fungus, Raffaelea quercivora, 21 isolates of the fungus were collected from areas where the mass mortality of oak trees has occurred. Additional 5 fungal isolates that were type species of R.quercivora were also used for comparison of the characters described among these isolates. The size of conidial spores was 1.8-3.1×3.3-8.1 μm for in the type species and 1.4-5.4×3.0-10.3 μm for the 21 isolates. The fungal isolates used for the morphological characterizations were further applied for DNA analyses focusing on both an internal transcribed spacer (ITS) region and a D1/D2 region in the 28s region of ribosomal DNA. The 21 isolates collected from the damage sites were quite similar in morphological and molecular traits with those of the type species suggesting that the isolates were identical to R.quercivora. We added 114 fungal isolates that supposed to be R.quercivora obtained from wilting or dead … More trees at damage sites of the mass mortality of oak trees in Japan. They were applied for random fragment length polymorphism (RFLP) analyses with three enzymes. Seventy-eight of 114 were successful for the RFLP analyses in all the enzymes, and the RFLP pattern was identical to that of the type species. These results indicate that R.quercivora is a pathogen of the mass mortality of oak trees and prevalent over damaged areas in Japan.We inoculated R.quercivora to the seedling of 4 Fagaceae species to determine the major factors associated with the susceptibility of the species to the fungus. After fungal inoculation, we measured the axial length of the discoloration and the areas of discolored and non-conducting sapwood on stem cross-sections. There were significant differences in the susceptibility among the tree species that was a strong correlation between the expansion of transverse discolored areas and the range of non-conductive sapwood areas formed.We measured morphological traits of ambrosia beetles, Platypus quercivorus that have mycangia retaining R.quercivora collected from Wakayama prefecture. The mating test of P.quercivorus was conducted for populations between Wakayama prefecture and Kyoto prefecture. Although the cross mating derived from the different populations were successful, the reproduction rate in the cross mating was lower than that within the same population. We further examined the effect of both the body size of adult beetles and the formation of galleries on reproduction successes by the cross mating between the populations obtained the two prefecture as above. Less

为了阐明病原真菌Raffaelea quercivora的形态和分子特征的差异，从发生大规模死亡的橡树的地区收集了21株真菌。另外5个真菌分离物的模式种的R.quercivora也被用于这些菌株之间所描述的特征进行比较。分生孢子大小为1.4-5.4×3.0-10.3 μm，模式种为1.8-3.1×3.3-8.1 μm。用于形态学表征的真菌分离物被进一步应用于DNA分析，其集中于核糖体DNA的28 s区域中的内部转录间隔区（ITS）区域和D1/D2区域。从受害地点收集的21个菌株在形态和分子特征上与模式种非常相似，表明这些菌株与R. quercivora相同。我们添加了114个真菌分离物，这些分离物可能是从萎蔫或死亡的栎类根霉中获得的 ...更多信息在日本橡树大量死亡的受害地点的树木。他们被应用于随机片段长度多态性（RFLP）分析与三种酶。114个中有78个在所有酶的RFLP分析中成功，并且RFLP模式与模式种的RFLP模式相同。这些结果表明，栎栎腐病菌是造成橡树大量死亡的病原菌，在日本的受害地区普遍存在。在真菌接种后，我们测量了变色的轴向长度和变色和不导电边材在茎横截面上的面积。有显着差异的树木品种之间的敏感性是一个很强的相关性之间的横向变色区的扩展和非导电边材领域形成的范围。我们测量的食虫虻，鸭嘴兽quercivorus的形态特征，有mycangia保留R.quercivora收集从和歌山县。在和歌山县和京都县之间的种群中进行了槲皮树的交配试验。不同种群间的杂交虽然成功，但其繁殖率低于同一种群内的繁殖率。通过上述两个县的种群间的杂交，我们进一步研究了成虫的体型和廊道的形成对繁殖成功率的影响。少