Research on high-degree utilization of genetic information on aquaculture fish

养殖鱼类遗传信息高度利用的研究

• 批准号: 15380134
• 负责人: OKAMOTO Nobuaki
• 金额: $ 9.86万
• 依托单位: Tokyo University of Marine Science and Technology (2004-2005)東京水産大学 (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380134/
• 关键词: Japanese flounder; genetic linkage map; microsatellite marker; breed improvement; anchor gene; synteny; chromosome preparation; 物理地図; FISH法; C-バンド; R-バンド

We produced a standard family of Japanese flounder, Paralichthys olivaceus, for genetic linkage maps. Connecting to a previously reported Japanese flounder linkage map with 111 microsatellite markers (Coimbra et al.,2003), a new linkage map was covered with 400 microsatellite markers, 144 SNP markers originated from EST, MHC gene and 5SrRNA gene. At the results of this study, MHC gene and 5SrRNA gene were located on Linkage group 13 and 23, respectively.Numbers of chromosomes in Japanese flounder have different opinions. We tested 40 fish originated from Hokkaido, Iwate, Akita, Yamagata, Niigata, Kanagawa and Ishikawa, and showed that all the fish have 2n=48 including the standard family for genetic linkage maps.Synteny to zebra fish and fugu fish was partially compared and some genes of Japanese flounder were conserved in both fishes, although the position made complexity.In order to compare the synteny between Japanese flounder and fugu fish and/or zebra fish, it is requested that anchor genes should be developed more.In addition, we identified a major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder, using the genetic linkage map.

我们制作了一个标准的日本牙鲽家族（Paralichthys olivaceus），用于遗传连锁图谱。与先前报道的具有111个微卫星标记的日本牙鲆连锁图谱(Coimbra et al.,2003)连接，新的连锁图谱覆盖了400个微卫星标记、144个源自EST、MHC基因和5SrRNA基因的SNP标记。本研究结果表明，MHC基因和5SrRNA基因分别位于连锁群13和23上。牙鲆的染色体数目存在不同的看法。我们测试了来自北海道、岩手县、秋田县、山形县、新泻县、神奈川县和石川县的40种鱼类，结果表明，包括遗传连锁图谱标准家族在内，所有鱼类都具有2n=48。对斑马鱼和河豚鱼的同线性进行了部分比较，发现牙鲆的一些基因在两种鱼类中都被保留，尽管位置变得复杂。 比较日本牙鲆与河豚鱼和/或斑马鱼之间的同线性，要求进一步开发锚定基因。此外，我们利用遗传连锁图谱鉴定了日本牙鲆对淋巴囊肿病抗性的一个主要遗传位点。

项目成果

Isolation and characterization of new microsatellite markers from the Japanese flounder (Paralichthys olivaceus)

日本牙鲆（Paralichthys olivaceus）新微卫星标记的分离和表征

• 发表时间: 2006
• 期刊: Molecular Ecology Notes 6
• 作者: C.Castano Sanchez;K.Fuji;K.KobayashiI;O.Hsasegawa;E.Ohara;A.OzakiI;M.R.M.Coimbura;T.Sakamoto;N.Okamoto
• 通讯作者: N.Okamoto

Maria R.M.Coimbra: "A genetic linkage map of the Japanese flounder, Paralichthys olivaceus"Aquaculture. 220. 203-218 (2003)

Maria R.M.Coimbra：“日本比目鱼、Paralichthys olivaceus 的遗传连锁图”水产养殖。

Identification of the single major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder(Paralichthys olivaceus)

控制日本牙鲆（Paralichthys olivaceus）淋巴囊肿病抗性的单一主要遗传位点的鉴定

• 发表时间: 2006
• 期刊: Aquaculture 254
• 作者: Kanako Fuji;Kazunobu Kobayashi;Osamu Hasegawa;M.R.M.Coimbra;Takashi Sakamoto;Nobuaki Okamoto
• 通讯作者: Nobuaki Okamoto

Identification of the single major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder (Paralichthys olivaceus)

控制日本牙鲆（Paralichthys olivaceus）对淋巴囊肿病抗性的单一主要遗传位点的鉴定

• 发表时间: 2006
• 期刊: Aquaculture 254
• 作者: Kanako Fuji;Kazunobu Kobayashi;Osamu Hasegawa;M.R.M.Coimbra;Takashi Sakamoto;Nobuaki Okamoto
• 通讯作者: Nobuaki Okamoto

Identification of the single major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder (Paralichthys olivaceus).

鉴定控制日本牙鲆（Paralichthys olivaceus）对淋巴囊肿病抗性的单一主要遗传位点。

• 发表时间: 2006
• 期刊: Aquaculture (On-line)
• 作者: Kanako Fuji;Kazunobu Kobayashi;Osamu Hasegawa;M.R.M.Coimbra;Takashi Sakamoto;Nobuaki Okamoto
• 通讯作者: Nobuaki Okamoto