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Genetic analysis of measles viruses isolated in Japan and mechanism of transmission-role of antibodies induced by vaccination

日本分离的麻疹病毒基因分析及传播机制——疫苗接种诱导抗体的作用

基本信息

批准号：
15390211
负责人：
ITOH Masae
金额：
$ 8.45万
依托单位：
Nagahama Institute of Bio-Science and Technology (2004-2006)Osaka Prefectural Institute of Public Health (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390211/
关键词：
measles virus vaccine antibody genome analysis H gene N gene cell fusion diversity 多核巨細胞 F遺伝子

项目摘要

Nucleotide sequences of the H and N genes of measles viruses isolated in Japan since 1980s were determined and compared with those obtained from databases. By examining accumulation of the mutations and genetic development of the genes, the origin of each virus was assumed and the affect of antibodies acquired by vaccination on the rate of accumulation of the mutations was discussed.Type D3 virus : Type D3 viruses were prevailing in the late 1980s and the early 1990s, which were replaced by Type D5 viruses and emerged again in 1997. Re-emerging D3 viruses originated from the Guam.USA/94 like strain and mutated into two lineages in Japan.Type D5 virus : Type D5 viruses in the early 2000s were supposed to be the progenitor of type D5 viruses in the early 1990s and the most probable ancestor was the Palau.BLA/93 like virus. In 2007, the measles virus infection burst around Tokyo and is now spreading all over Japan. The isolated viruses from the epidemic belong to the different lineage of type D5 from that of type D5 viruses in the early 2000s, to the same lineage with MVi/Queensland.AU/37.03.Type H1 virus : Type H1 viruses have been isolated in Japan since 1999. Type H1 viruses isolated in Japan could be divided into two groups and there is a possibility that two kinds of type H1 virus possessing different gene sequences invaded into Japan.Isolates from vaccinated patients : H and N genes of the viruses from the vaccinated patients were compared with those of isolates from the same epidemic. We could not confirm the increased accumulation of mutations with the viruses isolated from vaccinated patients.Diversity of measles virus : A strain of measles virus derived from a patient revealed not to be genetically single but to be a mixed population.

测定了 20 世纪 80 年代以来日本分离的麻疹病毒的 H 和 N 基因的核苷酸序列，并与从数据库中获得的序列进行了比较。通过检查突变的积累和基因的遗传发育，推测了每种病毒的起源，并讨论了通过疫苗接种获得的抗体对突变积累率的影响。 D3型病毒：D3型病毒在20世纪80年代末和90年代初盛行，被D5型病毒取代并于1997年再次出现。重新出现的D3病毒起源于 Guam.USA/94 样病毒株，在日本突变为两个谱系。 D5 型病毒：2000 年代初的 D5 型病毒被认为是 1990 年代初 D5 型病毒的祖先，最可能的祖先是 Palau.BLA/93 样病毒。 2007年，麻疹病毒感染在东京周边爆发，目前正在日本全国蔓延。此次疫情中分离到的病毒与2000年代初的D5型病毒属于不同的D5型病毒谱系，与MVi/Queensland.AU/37.03属于同一谱系。 H1型病毒：日本自1999年起就分离出H1型病毒。日本分离到的H1型病毒可分为两类，两种H1型病毒有可能具有不同的特征。基因序列入侵日本。来自接种疫苗的患者的分离株：将来自接种疫苗的患者的病毒的 H 和 N 基因与来自同一流行病的分离株进行比较。我们无法证实从接种疫苗的患者中分离出的病毒突变积累是否增加。麻疹病毒的多样性：来自患者的麻疹病毒株显示出其基因并非单一，而是一个混合群体。