Restoration of renal microcirculation by eNOS gene transfection for overcoming the antibody-mediated rejection and achievement of long-term renal allograft survival

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1. From the clinicopathological observation of antibody-mediated rejection in living donor ABO-incompatible kidney transplantation and ischemia-reperfusion injury in deceased donor kidney transplantation, We had analyzed and evaluated the process of the microcirculation injury and microvascular endothelial cell damage.2. We had established the GFP(+) to (-) rat bone marrow transplantation model and evaluated the role of The bone marrow derived endothelial progenitor cells in the regeneration of the injured microvascular Cells in renal ischemia-reperfusion injury.3. We have revealed that the bone marrow derived endothelial cells have migrated and recovered the injured endothelial cells in renal ischemia-reperfusion injury.It was approved not only by static immunohinstopathological method but also by dynamic direct observation by real-time phase contrast microscopy.4. We have also established in vitro eNOS gene transfection into cultured vascular endothelial cells, However, transplantation and restoration of injured endothelial cells by eNOS transfectant endothelial cells in "in vivo" experimental model could not be established.5.These result will be useful for the clinical renal preservation and regeneration therapy for antibody-mediated rejection in ABO-incompatible kidney transplantation and ischemia-reperfusion injury in deceased donor kidney transplantation.