Analysis of Cementum Formation in Periodontal Regeneration with Bone Morphogenetic Proteins for Development of Periodontal Regeneration Therapy.

1)将重组人骨形态发生蛋白-2 （rhBMP-2）以聚乳酸-聚乙醇酸共聚物/明胶海绵（PGS）载体应用于成年犬手术所致两壁和三壁牙周缺损。组织统计学结果显示，rhBMP-2促进三壁牙周缺损的骨形成。2)对6只beagle犬在双侧前磨牙牙根周围行水平周向牙周缺损手术治疗。用弹性结扎法紧绕缺损根部，以抑制完整牙周韧带组织细胞的冠状迁移。牙釉质基质衍生物（EMD）用于实验侧，载体单独用于对侧作为对照。rhBMP-2/PGS应用于试验点和对照点。术后12周，进行组织计量学分析。两组间骨质形成量差异无统计学意义。EMD组缺损底部与强直根尖端之间的距离明显大于对照组。3)将携带核心结合因子α -1 （Cbfa1）基因的腺病毒吸附在多孔β -三磷酸钙阻滞剂上，应用于成年犬水平周周缺损的修复。由于所有牙块均在愈合期暴露于口腔，因此本研究无法评估Cbfa1对牙周再生的影响。4)对6只成年犬进行上颌中侧实验性牙周炎的诱导。在缺陷处注入磷酸钙水泥（CPC）。对侧未移植缺损作为对照。术后12周，用组织学方法评估牙周组织愈合情况。实验部位新骨、新骨质、新结缔组织的附着和粘连明显增强。CPC对实验性牙周炎犬牙周缺损的创面愈合稳定，促进牙周再生。少

1) Recombinant human bone morphogenetic protein-2 (rhBMP-2) with polylactic acid-polyglycolic acid copolymer/gelatin sponge (PGS) carrier was applied on two-wall- and three-wall surgically created periodontal defects on adult dogs. The histometric results suggested that rhBMP-2 enhanced bone formation in three-wall-periodontal defects.2) Horizontal circumferential periodontal defects were surgically created around the root of premolar bilaterally in six beagle dogs. An elastic ligature was placed tightly around the root at the base of the defect in order to inhibit coronal migration of cells from intact periodontal ligament tissue. Enamel matrix derivative (EMD) was applied in the experimental side and carrier alone was applied in the contra-lateral side as control. rhBMP-2/PGS was applied in the experimental and control sites. Twelve weeks after the surgery, histmetric analysis was performed. There were not statistically significant differences in the amount of cementum formation betw … More een the groups. The distance between the bottom of the defect and apical terminal of ankylosis was significantly greater in EMD group compared to control.3) Adenovirus with core binding factor alpha-1 (Cbfa1) gene adsorbed to porous beta-tri-calcium phosphate block was applied on horizontal circumferential periodontal defects in adult dogs. As all blocks were exposed to oral cavity in healing period, it was impossible to evaluate the effect of Cbfa1 on periodontal regeneration in this study.4) Experimental periodontitis was induced on the mesial side of maxillary canines in six adult dogs. Calcium phosphate cement (CPC) was injected in the defects. Non-grafted defects on the contralateral side served as controls. Twelve weeks after surgery, periodontal tissue healing was evaluated histometrically. New bone, new cementum, and new connective tissue attachment and adhesion were significantly enhanced in the experimental sites. CPC provided stable wound healing and enhanced periodontal regeneration in periodontal defects in dogs with experimental periodontitis. Less