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Analysis of Cementum Formation in Periodontal Regeneration with Bone Morphogenetic Proteins for Development of Periodontal Regeneration Therapy.

用骨形态发生蛋白分析牙周再生中牙骨质的形成，以开发牙周再生疗法。

基本信息

批准号：
15390643
负责人：
KINOSHITA Atsuhiro
金额：
$ 2.75万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

1) Recombinant human bone morphogenetic protein-2 (rhBMP-2) with polylactic acid-polyglycolic acid copolymer/gelatin sponge (PGS) carrier was applied on two-wall- and three-wall surgically created periodontal defects on adult dogs. The histometric results suggested that rhBMP-2 enhanced bone formation in three-wall-periodontal defects.2) Horizontal circumferential periodontal defects were surgically created around the root of premolar bilaterally in six beagle dogs. An elastic ligature was placed tightly around the root at the base of the defect in order to inhibit coronal migration of cells from intact periodontal ligament tissue. Enamel matrix derivative (EMD) was applied in the experimental side and carrier alone was applied in the contra-lateral side as control. rhBMP-2/PGS was applied in the experimental and control sites. Twelve weeks after the surgery, histmetric analysis was performed. There were not statistically significant differences in the amount of cementum formation betw … More een the groups. The distance between the bottom of the defect and apical terminal of ankylosis was significantly greater in EMD group compared to control.3) Adenovirus with core binding factor alpha-1 (Cbfa1) gene adsorbed to porous beta-tri-calcium phosphate block was applied on horizontal circumferential periodontal defects in adult dogs. As all blocks were exposed to oral cavity in healing period, it was impossible to evaluate the effect of Cbfa1 on periodontal regeneration in this study.4) Experimental periodontitis was induced on the mesial side of maxillary canines in six adult dogs. Calcium phosphate cement (CPC) was injected in the defects. Non-grafted defects on the contralateral side served as controls. Twelve weeks after surgery, periodontal tissue healing was evaluated histometrically. New bone, new cementum, and new connective tissue attachment and adhesion were significantly enhanced in the experimental sites. CPC provided stable wound healing and enhanced periodontal regeneration in periodontal defects in dogs with experimental periodontitis. Less

1)以聚乳酸-聚乙醇酸共聚物/明胶海绵（PGS）为载体，将重组人骨形态发生蛋白-2（rhBMP-2）应用于成年犬牙周组织缺损的修复。组织学测量结果表明rhBMP-2促进了牙周三壁缺损的成骨。2）在6只比格犬的双侧前磨牙根部周围建立水平周向牙周缺损。在缺损的根部用弹性结扎线紧紧地绑住牙根，以抑制细胞从完整的牙周韧带组织向冠状面迁移。实验侧应用牙釉质基质衍生物（EMD），对侧单独应用载体作为对照。rhBMP-2/PGS应用于实验和对照部位。术后12周进行组织学分析。牙骨质形成量与对照组无统计学差异 ...更多信息即使是团体。实验组牙周组织缺损底部与根尖端的距离明显大于对照组。3）将携带核心结合因子α 1（Cbfa 1）基因的腺病毒载体吸附于多孔β-磷酸三钙（β-tri-calcium phosphate，β-tri-calcium phosphate）上，应用于成年犬牙周水平周向缺损。由于愈合期所有材料均暴露于口腔中，因此无法评价Cbfa 1对牙周组织再生的影响。4）在6只成年犬上颌尖牙近中侧建立实验性牙周炎模型。缺损处注入磷酸钙骨水泥（CPC）。对侧的非移植缺损作为对照。手术后12周，牙周组织愈合进行了组织计量学评价。实验部位新骨、新牙骨质和新结缔组织附着和粘附显著增强。CPC在实验性牙周炎犬牙周缺损中具有稳定的伤口愈合和促进牙周再生的作用。少