損傷脳における炎症性細胞集簇の病態的解明

受损大脑中炎症细胞簇的病理学阐明

• 批准号: 15790780
• 负责人: 久保 慶高
• 金额: $ 2.3万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15790780/
• 关键词: 多核白血球; 単球・大食細胞; 反応性アストロサイト; 脳損傷; 接着分子; 脳動脈瘤破裂によるくも膜下出血; 脳血管攣縮; トロンビン; アンチトロンビンIII; 単球系炎症細胞; vimentin陽性アストロサイト; GFAP陽性アストロサイト

1)ラット大脳皮質切創損傷モデルで脳損傷部位にゼラチンを留置し、生理食塩水(対照群、n=20)、AT III 500U/kg/day(治療群、n=20)を腹腔内圧ポンプにて持続的投与した。脳損傷作成1、3、5、7日後、ゼラチンと創縁における炎症反応とダリア反応をHE染色ならびにmonocytes/macrophages (Mo/MΦ)、GFAP、vimentinによる免疫化学染色で組織学的に比較検討を行った。【結果】治療群は対照群に比較して、損傷後1、3日目における多核白血球と3、5、7日目におけるMo/MΦ陽性細胞を有意に抑制した(p<0.05)。また、GFAP陽性アストロサイトの増殖は認めなかったが、3、5、7日目におけるvimentin陽性アストロサイトの増殖を有意に抑制した(p<0.05)。以上の結果からAT IIIは、脳損傷部位近傍における炎症性細胞の浸潤やvimentin陽性アストロサイトを抑制することにより、脳損傷後の組織反応や修復過程で生じる瘢痕化を抑制する可能性が示唆された。2)脳動脈瘤破裂によるくも膜下出血患者18人ならびに未破裂脳動脈瘤患者4人において、血漿のL-セレクチン、P-セレクチン、E-セレクチン、ICAM-1、VCAM-1、MCP-1、高感度CRPをくも膜下出血発症2日(48時間)以内と7日後、未破裂脳動脈瘤患者では術前と術後7日目に測定した。対象をA群(SVS+, AVS+)4人、B群(SVS-, AVS+)3人、C群(SVS-, AVS-)11人、D群(未破裂脳動脈瘤患者)4人に分けた。SVS (symptomatic vasospasm :脳血管撃縮による脳虚血症状が出現)、AVS (angiographical vasospasm :脳血管撮影またはCTAの所見で50%以上の脳血管撃縮があり)。A群、B群、C群はD群に比べて血漿のL-セレクチン、P-セレクチン、E-セレクチン、ICAM-1、VCAM-1、MCP-1、高感度CRPが有意に高かった。また、A群はB群、C群に比べて発症2日(48時間)以内のトセレクチンと発症2日(48時間)以内と7日後のVCAM-1が有意に高かった。白血球の捕捉・遊走による循環障害、白血球の脳内浸潤による脳組織障害によって、<も膜下出血後に脳虚血症状が出現した可能性が示唆された。

1) in the abdominal cavity, the abdominal cavity was injected into the abdominal cavity and the abdominal cavity was injected into the abdominal cavity. 1) the skin was cut, the skin was cut, and the parts of the body were placed in the abdominal cavity, physiological water (Zhaoqun, nong20), AT III 500U/kg/day (treatment group, 20). On the 1st, 3rd, 5th and 7th day, the histology of immuno-chemical staining of monocytes/macrophages (Mo/M Φ), GFAP and vimentin was detected by immuno-chemical staining. [results] compared with the control group, the number of multinucleated white blood cells on the 1st and 3rd day after treatment was significantly higher than that on the 3rd, 5th and 7th day after treatment. The results showed that Mo/M Φ cells were intentionally suppressed. The colonization was intentionally suppressed on the 3rd, 5th and 7th day, and the vimentin was intentionally inhibited on the 3rd, 5th and 7th day. Results the above results showed that the inflammatory cells were immersed in the AT III, the inflammatory cells were immersed in the inflammatory cells, the inflammatory cells were immersed in the inflammatory cells, the inflammatory cells were immersed in the inflammatory cells, the inflammatory cells were immersed in the inflammatory cells, the inflammatory cells were immersed in the inflammatory cells, the inflammatory cells were immersed in the inflammatory cells, the inflammatory cells were immersed in the inflammatory cells, the inflammatory cells were immersed in the inflammatory cells, the inflammatory cells were immersed in the inflammatory cells, the inflammatory cells were immersed in the inflammatory cells, and the tissue tissue was used to reduce the possibility of scar formation in the course of repair. 2) 18 patients with submembranous hemorrhage of ruptured tumor, 4 patients with unruptured tumor, 4 patients with non-ruptured tumor, 4 patients with unruptured tumor, 4 patients with There were 4 patients in group A (SVS+, AVS+), 3 in group B (SVS-, AVS+), 11 in group C (SVS-, AVS-) and 4 in group D (patients with unruptured motor tumor). SVS (symptomatic vasospasm: vascular deficiency symptoms), AVS (angiographical vasospasm: vascular shadows, CTA). Group A, group B, group C and group D were significantly higher than those of group A, group B, group D, group B, group D, group A, group B, group C, group D, group A, group B, group C. Group A, group B and group C were diagnosed within 2 days (48 hours), and VCAM-1 was intended to be high within 7 days. The possibility of the appearance of deficiency blood symptoms after submembrane hemorrhage in white blood cells was detected by white blood cell capture, white blood cell walking, white blood cell impregnation, tissue damage and lt;.

项目成果

久保慶高: "Clinical Neuroscience"中外医学社. 2 (2003)

久保嘉孝：《临床神经科学》中外医学社 2 (2003)。

Kakino S: "Treatment of vertebral artery aneurysms with posterior inferior cerebellar artery-posterior Inferior cerebellar artery anastomosis combined with parent artery occlusion."Surg Neurol.. 61(2). 185-189 (2004)

Kakino S：“小脑后下动脉-小脑后下动脉吻合联合载瘤动脉闭塞治疗椎动脉瘤。”Surg Neurol.. 61(2)。

Granulomatous change of the pituitary stalk caused by Rathke's cleft cyst : a case report

Rathke裂囊肿引起的垂体柄肉芽肿性改变：一例报告

• 发表时间: 2003
• 期刊: No Shinkei Geka 31(11)
• 作者: Komoribayashi N;Kubo Y et al.
• 通讯作者: Kubo Y et al.

二次性頭痛の鑑別診断

继发性头痛的鉴别诊断

• 发表时间: 2004
• 作者: Otawara Y;Kubo Y et al.;久保慶高
• 通讯作者: 久保慶高

Treatment of vertebral artery aneurysms with posterior inferior cerebellar artery-posterior inferior cerebellar artery anastomosis combined with parent artery occlusion

• DOI: 10.1016/j.surneu.2003.06.001
• 发表时间: 2004-02-01
• 期刊: SURGICAL NEUROLOGY
• 作者: Kakino, S;Ogasawara, K;Ogawa, A
• 通讯作者: Ogawa, A