Dynamics of synapse and exocytosis studied with two-photon excitation

用双光子激发研究突触和胞吐作用的动力学

• 批准号: 16002012
• 负责人: KASSAI Haruo
• 金额: $ 385.22万
• 依托单位: The University of Tokyo (2005-2008)National Institute for Physiological Sciences (2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Specially Promoted Research
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16002012/
• 关键词: 生物・生体工学; シグナル伝達; 生理学; 糖尿病; 脳・神経

Using two-photon microscope and uncaging of caged-glutamate compounds, we have revealed that rapid motilities of dendritic spines underlie plasticity of excitatory synaptic transmission in the cerebral cortex. We have visualized how actin reorganization underlies the motilities, and how spine sizes can be maintained over days even though the synapses are living small structures, and cannot avoid the natural fluctuations. We have developed a new caged-GABA compound, which can be used simultaneously with caged-glutamate compound. A new principle for visualization of exocytosis with two-photon microscope has been established, and applied to analyze conformational changes of responsible molecules.

用双光子显微镜和笼状谷氨酸化合物的去势，我们揭示了大脑皮层兴奋性突触传递可塑性的基础是树突棘的快速运动。我们已经可视化了肌动蛋白重组是如何支撑运动的，以及如何在突触是活的小结构且无法避免自然波动的情况下，脊柱大小如何在几天内保持不变。我们开发了一种新的笼状GABA化合物，它可以与笼状谷氨酸化合物同时使用。建立了用双光子显微镜显示胞吐作用的新原理，并将其应用于分析相关分子的构象变化。

项目成果

Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla

• DOI: 10.1038/sj.emboj.7600983
• 发表时间: 2006-02-22
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Kishimoto, T;Kimura, R;Kasai, H
• 通讯作者: Kasai, H

2光子励起顕微鏡の特徴と医学応用

双光子激发显微镜的特点及医学应用

• 发表时间: 2008
• 作者: Noriko Takahashi;Hiroyasu Hatakeyama;Haruo Kasai;河西春郎
• 通讯作者: 河西春郎

Structural basis of long-term potentiation in single dendritic spines

• DOI: 10.1038/nature02617
• 发表时间: 2004-06-17
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Matsuzaki, M;Honkura, N;Kasai, H
• 通讯作者: Kasai, H

Two-photon imaging, uncaging and photoactivation of dendritic spines.

树突棘的双光子成像、释放和光激活。

• 发表时间: 2008
• 作者: Kasai;H.
• 通讯作者: H.

Dendritic spine structures critical for spine functions and plasticity.

树突棘结构对于脊柱功能和可塑性至关重要。

• 发表时间: 2005
• 作者: Kasai;H.;H. Kasai;H. Kasai;H. Kasai;H. Kasai
• 通讯作者: H. Kasai