Acid-sensing ion channel-1 b in the stereocilia of mammalian cochlear hair cells

哺乳动物耳蜗毛细胞静纤毛中的酸敏感离子通道 1 b

• 批准号: 16390490
• 负责人: UGAWA Shinya
• 金额: $ 7.87万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390490/
• 关键词: acid-sensing ion channel; amiloride; cochlea; mechano-electrical transduction; mouse; immunoelectron microscopy; in situ hybridization; ASIC1b; TRP; OTRPC4; ASIC; Western blot

We investigated whether amiloride-blockable proton-gated cation channels ASIC1 a (acid-sensing ion channel-1 a) and ASIC1 b are expressed in the stereocilia of mouse cochlear hair cells. In situ hybridization studies showed that ASIC1b transcripts, but not ASlC1 a transcripts, were expressed in the inner and outer hair cells. Fluorescent immunohistochemical and immunogold electron microscopic analyses revealed that the ASIC1b channels were located at the insertions of the stereocilia into the hair cells. Our findings provide a novel molecular key to the understanding of cochlear physiology and pathophysiology.

我们研究了阿米洛利可阻断的质子门控阳离子通道ASIC1a(酸敏离子通道-1a)和ASIC1b是否在小鼠耳蜗毛细胞的立体纤毛中表达。原位杂交研究表明，内、外毛细胞表达ASIC1b转录本，而不表达AS1C1a转录本。荧光免疫组织化学和免疫金电子显微镜分析表明，ASIC1b通道位于毛细胞的立体纤毛连接处。我们的发现为理解耳蜗生理学和病理生理学提供了一把新的分子钥匙。

项目成果

Acid-sensing ion channel-1b in the stereocilia of mammalian cochlear hair cells.

哺乳动物耳蜗毛细胞静纤毛中的酸敏感离子通道 1b。

• 发表时间: 2007
• 期刊: 第112回日本解剖学会総会 (学会発表)
• 作者: Hiyama E;et al.;Honda MJ;佐々木文章ほか;Sasaki F et al.;Ito A;Tanaka K;Hongo T;Ito A;Ito A;Niwa Y.(et al.);Ito Y.(et al.);Ito A.(et al.);Komeima K. (et al.);Kobayashi C. (et al.);Kito K.(et al.);Suzuki A.(et al.);Ishikawa K. (et al.);Chen GY.(et al.);Lin J.(et al.);Ueno S.(et al.);鵜川 眞也
• 通讯作者: 鵜川 眞也