The role of motile activity of mesenchymao stem cells in regeneration of musculoskeletal organs

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We have established 10 cultured cell lines of mesenchymal stem cells from bone marrow in patients receiving a surgical treatment at pelvis, and found that they differentiate into bone and cartilage. For the culture autologous serum was superior to fetal bovine serum in the cell ability to proliferate and move. Also in an animal model, a specific hyaluronic acid was found to have excellent effect on cartilage regeneration as a scaffold for stem cells, but the effect being independent of CD44. Furthermore, Autocrine motility factor (AMF) play a role in meniscus regeneration induced by surrounding synovial stem cells. On the other hand, expression of RNA message rather than protein was related to malignancy in bone and soft tissue tumors, and the protein was regulated by intracellular degradation. This was also found in lung cancers. The AMF molecule was found to bind to poly(ADP-ribose) polymerase family 14. These results play an important role in the elucidation and promotion of molecular biology in cell motility of mesenchymal stem cell, from clinical and basic points of view.