Osteochondral regeneration using autologous cell construct with β TCP
使用带有 β TCP 的自体细胞构建体进行骨软骨再生
基本信息
- 批准号:16591497
- 负责人:
- 金额:$ 2.37万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2004
- 资助国家:日本
- 起止时间:2004 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We established a new method to make cell only construct on TCP to repair osteochondral defect.Mesenchymal stem cells (MSCs) were obtained from the iliac crest of Japanese-white-rabbit. Monolayer expanded cells were mold into a cylinder of 4.8mm in diameter and 4mm in height on TCP. The autologous labeled-MSC plug was implanted with TCP in the patellofemoral groove of the rabbit. The knees were immobilized for one week. The rabbits were sacrificed at 3, 6, 12, 24, and 52 weeks after operation. The specimens were evaluated histologically and scanned with micro CT.In gross appearance of the cartilage at 6 weeks, most of the surface of the defect was covered with cartilage-like tissue. Smooth surfaces were maintained in regenerated cartilage at one year. Histologically, columnar distribution of the chondrocyte was observed in regenerated hyaline cartilage. In the subchondral bone level, endochondral ossification occurred at the peripheral area of the transplants, and small area in the central remained hyaline cartilage. Micro CT showed further ossification in subchondral bone level. Confocal microscopy detected the labeled cells both in cartilage layer and subchondral bone layer.The current study suggests that this cell-delivery system can be used for treatment of osteochondral defect and possibly for osteoarthritis.
本研究建立了一种单纯细胞构建于TCP上修复骨软骨缺损的新方法,从日本白兔髂嵴中分离获得间充质干细胞(MSCs)。将单层扩增的细胞在TCP上模制成直径4.8mm、高4mm的圆柱体。将标记的自体MSC栓与TCP一起植入兔髌股沟内。膝盖固定一周。分别于术后3、6、12、24、52周处死动物。术后6周,大体观察软骨缺损区表面大部分被软骨样组织覆盖。在一年时再生软骨中保持光滑表面。组织学观察,再生透明软骨中软骨细胞呈柱状分布。在软骨下骨水平,移植物的周边区域发生软骨内骨化,中央区域的小区域保持透明软骨。显微CT显示软骨下骨水平进一步骨化。共聚焦显微镜观察到标记细胞分布于软骨层和软骨下骨层,提示该细胞传递系统可用于骨软骨缺损的治疗,并有可能用于骨关节炎的治疗。
项目成果
期刊论文数量(71)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Tracking implanted mesenchymal stem cells in vivo with nano-crystal reagents
使用纳米晶体试剂追踪体内植入的间充质干细胞
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Nakayama K;Matsuda S;Y Akasaki;K Tanaka;H Miura;Y Iwamoto
- 通讯作者:Y Iwamoto
Novel cell delivery system without carrier vehicle : Regeneration of rabbit ostes-chondral defect with autologous mesenchymal stem cell
无载体的新型细胞递送系统:自体间充质干细胞再生兔骨软骨缺损
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Nakayama K;Matsuda S;Y Akasaki;K Tanaka;H Miura;Y Iwamoto
- 通讯作者:Y Iwamoto
Contact stress at the post-cam mechanism in posterior stabilized total knee arthro plasty
后稳定全膝关节成形术中后凸轮机构的接触应力
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Nakayama K;Matsuda S;et. al.
- 通讯作者:et. al.
The oblique posterior condylar radiographic view to evaluate the posterior femoral condyle following total knee arthroplasty
斜后髁X线片评估全膝关节置换术后股骨后髁
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Miura H;Matsuda S;et. al.
- 通讯作者:et. al.
Overview of cartilage tissue engnieering
软骨组织工程概述
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Nakayama K;Matsuda S;et. al.
- 通讯作者:et. al.
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MATSUDA Shuichi其他文献
MATSUDA Shuichi的其他文献
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{{ truncateString('MATSUDA Shuichi', 18)}}的其他基金
The role of small GTP-binding proteins and cytoskeleton in the development of osteoarthritis and chondrogenesis
小 GTP 结合蛋白和细胞骨架在骨关节炎和软骨形成发展中的作用
- 批准号:
24592267 - 财政年份:2012
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The anti-inflammatory effects of stat in on osteoarthritis
stat对骨关节炎的抗炎作用
- 批准号:
21591949 - 财政年份:2009
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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