Investigation of contrast ultrasound imaging with microbubble contrast agents for diagnosis of sentinel lymph node metastasis using esophageal cancer animal model

超声造影剂由微泡组成。Sonazoid[R]由全氟丁烷气体作为内部气体组成，并具有磷脂酰丝氨酸外壳。前哨淋巴结经皮下及粘膜下注射造影剂后显影。淋巴结超声成像的适当条件是：1）当MI> 0.4时，需要比循环血液中流动的微泡的造影剂成像更高的MI（机械指数）。相位调制谐波成像技术比振幅调制谐波成像技术更适用于枯否细胞吞噬微泡的成像. HSP（热休克蛋白）的表达，而超声暴露和吞噬的微泡被破坏是依赖于发射的ultrason.Lymph结肿胀模型MI揭示了不同的模式之间的对比成像淋巴结转移的VX 2肿瘤和炎症淋巴结肿胀细菌感染。

Ultrasound contrast agents are composed of microbubble. Sonazoid[R] is composed of perfluorobutane gas as the inner gas and it has phosphatigyl serine outer shell. Sentinel lymph node is opacified with contrast agent when it is administered into the subcutaneous and submucosal tissues.The results of the projects are as follows ;1. Proper conditions for ultrasound imaging of lymph nodes are 1) higher MI (mechanical index) is necessary than contrast imaging of microbubbles flowing in the circulating blood as MI>0.4.2. Phase modulation harmonic imaging technique is more proper for phagocytosed microbubbles in the Kupffer cells than amplitude modulation harmonic imaging technique.3. HSP (heat shock protein) expression while ultrasound is exposed and phagocytosed microbubbles are destroyed is dependent on the MI of the emitted ultrasound.Lymph node swelling models revealed different patterns of contrast imaging between lymph node -'metastatic from VX2 tumor and inflammatory lymph node swelling from bacterial infection.