The Role of CEACAM1 (CEA-related cell adhesion molecule 1) in vascular morphogenesis and stabilization

CEACAM1（CEA相关细胞粘附分子1）在血管形态发生和稳定中的作用

• 批准号: 5347735
• 负责人: Professor Dr. Süleyman Ergün
• 金额: --
• 依托单位: II. Medizinische Klinik und Poliklinik (Onkologie, Hämatologie, Knochenmarktransplantation mit Abteilung für Pneumologie)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2003-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5347735?language=en
• 关键词: Role; CEACAM1; CEA; related; cell

Angiogenesis is regulated by a net balance of angiogenic activators and inhibitors. Cell adhesion molecules are also involved in the different stages of angiogenesis, in particular of vascular morphogenesis. We recently charakterized soluble CEACAM1 (CEA-related cell adhesion molecule 1) as a pro-angiogenic factor and morphogenic effector of VEGF. Increased CEACAM1 expression was found in endothelial cells of new blood vessels but the influence of membrane-bound CEACAM1 on the expression of known angiogenic factors and the morphogenesis of capillaries is still unknown. The aim of this study is to explore the role of membran-bound CEACAM1 in angiogenesis using the following experimental models: 1. Adenoviral overexpression of CEACAM1 in vascular endothelial and smooth muscle cells which will be subsequently used a) for gene array analyses regarding the expression of known potent angiogenic factors, b) migration and in vitro wound assays and c) endothelial tube formation assay. 2. Local microinjection of CEACAM1-adenovirus in chicken chorioallantoic membrane to study the effects of gene delivered CEACAM1 in angiogenesis and vascular leakage. These experiments will provide us with new insights concerning the effects of membrane-bound CEACAM1 on the angiogenesis.

血管生成受血管生成激活剂和抑制物的净平衡调节。细胞黏附分子还参与血管生成的不同阶段，尤其是血管形态发生。我们最近鉴定了可溶性CEACAM1(CEA相关细胞黏附分子1)是一种促血管生成因子和形态生成效应因子。CEACAM1在新生血管内皮细胞中表达增加，但膜结合型CEACAM1对已知的血管生成因子表达和毛细血管形态发生的影响尚不清楚。本研究的目的是通过以下实验模型探讨膜结合型CEACAM1在血管生成中的作用：1.腺病毒介导的CEACAM1在血管内皮细胞和平滑肌细胞中的过表达，这将被用于a)已知的潜在血管生成因子的表达的基因芯片分析，b)迁移和体外创伤试验，以及c)内皮管形成试验。2.鸡绒毛膜尿囊膜局部注射CEACAM1-腺病毒，观察CEACAM1基因对血管生成和血管渗漏的影响。这些实验将为我们提供关于膜结合的CEACAM1对血管生成的影响的新的见解。