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Chemical ecological studies for egg discrimination in paper wasps

纸黄蜂卵辨别的化学生态学研究

基本信息

批准号：
17570017
负责人：
TSUCHIDA Koji
金额：
$ 2.44万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17570017/
关键词：
Polistes chinensis antennalis egg discrimination pheromone worker policing queen policing Polistes selleni mated workers

项目摘要

We analyzed the mechanism of egg discrimination in a paper wasp, Polistes chinensis antennalts, by using a GC-MS. Dufour gland was immersed in Hexan for l h, and egg was done for 30 min. The cuticular substances of each adult were extracted by washing with Hexan. We used HP-5MS column and temperatures were changed according to the program at 150 ℃ for 2 min, 7 ℃ for l min and 320 ℃ for 5min. There were many extracted hydrocarbons for each sample. The substances extracted from Dufour glands were overlapped with those from eggs, in particular 7 chemicals, namely, (1) 2-MeC_(28), (2) n-C29, (3) 11-, 13-, 15-MeC29, (4) 11-, 13-, 15-, MeC31, (5) 11-, 13-, 15-MeC33, (6) 13-, 15-, 17-MeC35, (7) 13-, 19-deMeC35. These substances were also extracted from the cuticle surfaces.In Polistes snelleai, workers develop their ovaries even when original queen is still alive on the nests. Using a population in Nagano prefecture, we revealed that (1) there was a few mated workers on nests even when original queens were still alive, (2) the mated workers did not develop their ovaries, probably due to physiological suppression by the queens, and could not oviposit, when the queens were on nests, (3) the mated workers became replacement queen when the queens disappeared from the nests, (4) there were not significant differences between colony sizes of queen-right colonies and those of orphan colonies. The last finding strongly suggested that mated workers function as queens and colonies grow well even when queens disappeared. Nliorosatellite DNA analyses revealed that there were colonies with several genetic families, and suggested that polyandry and/or queen turnover take place in this species In addition, early males, which are mating partners of mated workers, function as gene pool in this species.

本文采用气相色谱-质谱联用技术，分析了中国小蜂（Polistes chinensis antennalts）成虫的卵识别机制。采用HP-5 MS色谱柱，程序升温，150 ℃ 2 min，7 ℃ 1 min，320 ℃ 5 min。每个样品都有许多提取的碳氢化合物。从杜氏腺中提取的物质与从卵中提取的物质重叠，特别是7种化学物质，即（1）2-MeC_（28），（2）n-C_（29），（3）11-，13-，15-MeC_（29），（4）11-，13-，15-，MeC_（31），（5）11-，13-，15-MeC_（33），（6）13-，15-，17-MeC_（35），（7）13-、19-deMeC35。这些物质也是从角质层表面提取的。在Polistes snelleai中，即使原来的女王仍然活着，工蜂也会发育卵巢。以长野县的一个种群为研究对象，揭示了：（1）即使在原始蜂王还活着的时候，也有少数工蜂在巢上交配;（2）当蜂王在巢时，交配后的工蜂卵巢不发育，可能是由于蜂王的生理抑制，不能产卵;（3）当蜂王从巢中消失时，交配后的工蜂成为替代蜂王，（4）右后群体与孤后群体的群体大小差异不显著。最后一个发现强烈表明，交配的工人作为皇后和殖民地生长良好，即使皇后消失。对该虫群体的DNA分析表明，该虫群体中存在多个遗传家族，并可能存在一妻多夫和/或蜂王更替现象。此外，该虫的早期雄虫是交配工蜂的交配伴侣，在该虫群体中起着基因库的作用。