Mechanisms of asymmetrical body formation during flatfish metamorphosis and prevention of malformed juveniles in seed production process

比目鱼变态过程中身体不对称形成机制及制种过程中畸形幼鱼的防治

• 批准号: 17580163
• 负责人: TAGAWA Masatomo
• 金额: $ 2.48万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17580163/
• 关键词: flatfish; metamorphic abnormality; slime flounder; Japanese flounder; starry flounder; thyroid hormone; timing hypothesis; left-right asymmetry; 変熊異常; ホシガレイ; 成長履歴解析; ALC多数回標識; マヌガレイ; 成長履歴; ヒラメ・カレイ類; 内分泌系の関与; 変態; 体色発現

For the mechanisms of asymmetrical body formation of flatfish development, we proposed a working hypothesis "timing hypothesis". From several lines of evidences, thyroid hormone induces blind side characteristics (white body color and migration of eye of the own side), and the tissue responsiveness to thyroid hormone exists differentially between right and left side of the body. Thyroid hormone has to be secreted during a limited time window when the responsiveness only presents on one side, in order to develop into normal juveniles. This project aimed to confirm the validity and generality of the hypothesis more in detail and for more species.In vivo appearance of all pigment cells during Japanese flounder development revealed the presence of three types of appearance manor as follows, 1) symmetrical larval type (larval type melanophore and xanthophore, and leucophore), 2) asymmetrical adult type (adult type melanophore and xanthophore), 3) symmetrical adult type (iridophore). For detailed study in future, in vitro culture for pigment cell ontogeny was attempted. At the end of this project term, we have established the culture condition in which all pigment cells (except for larval xanthophore) appear.Slime flounder (Microstomus achne) has extremely long, and starry flounder (Platichthys stellatus) has relatively short, pelagic life stages among flatfishes in Japan. For both species, thyroid hormone (T4, thyroxine) was added to the rearing tanks at various timings, and composition of normal, pseudoalbino, and ambicolorate, was examined after the completion of metamorphosis. Timing of T4 addition significantly altered the ratio, with a manor that can be explained by the timing hypothesis. In addition, the highest ratio of normal juveniles was observed in the tanks where the larvae metamorphosed at a similar timing to the sea. These results indicate the generality of the hypothesis to both species.

对于比目鱼发育不对称的体型形成机制，我们提出了一个工作假说--时间假说。从多条证据来看，甲状腺激素诱发盲侧特征(白色体色和一侧眼睛移位)，组织对甲状腺激素的反应性在身体的左右两侧存在差异。甲状腺激素必须在一个有限的时间窗口内分泌，当反应性只出现在一侧时，才能发育成正常的青少年。本项目旨在更详细地、更广泛地验证该假说的有效性和普适性。在牙鲆发育过程中，所有色素细胞的活体形态显示出三种外观庄园：1)对称型幼体(幼体类型的黑色素和黄体，以及白体)，2)不对称的成体类型(成体类型的黑素和黄体)，3)对称的成体类型(虹彩)。为进一步研究，对色素细胞个体发育的体外培养进行了尝试。在本项目期末，我们已经建立了所有色素细胞(除叶轮幼体外)都出现的培养条件。在日本的比目鱼中，粘质比目鱼(Microstomus Achne)具有极长的长度，星状比目鱼(Platichthys Stellatus)具有相对较短的远洋生活期。对这两个物种，在不同的时间向饲养池中添加甲状腺激素(T4，甲状腺素)，并在变态完成后检查正常、假白化和变色的组成。添加T4的时间显著改变了这一比例，这一点可以用时间假说来解释。此外，在幼虫变态的时间与大海相似的水箱中，观察到正常幼虫的比例最高。这些结果表明该假说对这两个物种都具有普遍性。

项目成果

変態期の左右性決定への甲状腺ホルモンの関与

甲状腺激素参与变态过程中侧向性的确定

• 发表时间: 2005
• 期刊: 日本水産学会誌 71
• 作者: Sergio Bolasina;Masatomo Tagawa;Yoh Yamashita;Masaru Tanaka;Nozomi Okada;Masatomo Tagawa;田川正朋
• 通讯作者: 田川正朋

ババガレイの変態異常に及ぼす甲状腺ホルモン投与時期の影響

甲状腺激素给药时机对比目鱼异常变态的影响

• 发表时间: 2007
• 作者: Tagawa;M;田川正朋
• 通讯作者: 田川正朋

ヒラメの変態期には質的に異なる2型(仔魚型・稚魚型)の黄色素胞が存在する

在牙鲆的变态阶段，有两种性质不同的黄色细胞（幼体型和幼年型）。

• 发表时间: 2007
• 作者: Tagawa;M;田川正朋;西川泰造;中村光男
• 通讯作者: 中村光男

IIIA.甲状腺ホルモン(P.1908-1929) (ホルモンハンドブック新訂eBook版、日本比較内分泌学会編)

IIIA.甲状腺激素（P.1908-1929）（激素手册新修订电子书版，日本比较内分泌学会编）

• 发表时间: 2007
• 作者: Aritaki;M;田川正朋;田川正朋
• 通讯作者: 田川正朋

Effect of stocking density on growth, digestive enzyme activity and cortisol level in larvae and juveniles of Japanese flounder, Paralichthys olivaceus

• DOI: 10.1016/j.aquaculture.2006.05.021
• 发表时间: 2006-09-08
• 期刊: AQUACULTURE
• 影响因子: 4.5
• 作者: Bolasina, Sergio;Tagawa, Masatomo;Tanaka, Masaru
• 通讯作者: Tanaka, Masaru