Mechanisms of asymmetrical body formation during flatfish metamorphosis and prevention of malformed juveniles in seed production process

比目鱼变态过程中身体不对称形成机制及制种过程中畸形幼鱼的防治

• 批准号: 17580163
• 负责人: TAGAWA Masatomo
• 金额: $ 2.48万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17580163/
• 关键词: flatfish; metamorphic abnormality; slime flounder; Japanese flounder; starry flounder; thyroid hormone; timing hypothesis; left-right asymmetry; 変熊異常; ホシガレイ; 成長履歴解析; ALC多数回標識; マヌガレイ; 成長履歴; ヒラメ・カレイ類; 内分泌系の関与; 変態; 体色発現

For the mechanisms of asymmetrical body formation of flatfish development, we proposed a working hypothesis "timing hypothesis". From several lines of evidences, thyroid hormone induces blind side characteristics (white body color and migration of eye of the own side), and the tissue responsiveness to thyroid hormone exists differentially between right and left side of the body. Thyroid hormone has to be secreted during a limited time window when the responsiveness only presents on one side, in order to develop into normal juveniles. This project aimed to confirm the validity and generality of the hypothesis more in detail and for more species.In vivo appearance of all pigment cells during Japanese flounder development revealed the presence of three types of appearance manor as follows, 1) symmetrical larval type (larval type melanophore and xanthophore, and leucophore), 2) asymmetrical adult type (adult type melanophore and xanthophore), 3) symmetrical adult type (iridophore). For detailed study in future, in vitro culture for pigment cell ontogeny was attempted. At the end of this project term, we have established the culture condition in which all pigment cells (except for larval xanthophore) appear.Slime flounder (Microstomus achne) has extremely long, and starry flounder (Platichthys stellatus) has relatively short, pelagic life stages among flatfishes in Japan. For both species, thyroid hormone (T4, thyroxine) was added to the rearing tanks at various timings, and composition of normal, pseudoalbino, and ambicolorate, was examined after the completion of metamorphosis. Timing of T4 addition significantly altered the ratio, with a manor that can be explained by the timing hypothesis. In addition, the highest ratio of normal juveniles was observed in the tanks where the larvae metamorphosed at a similar timing to the sea. These results indicate the generality of the hypothesis to both species.

对于比目鱼发育过程中不对称体形成的机制，我们提出了一个工作假说“时间假说”。从多个方面的证据来看，甲状腺激素诱导盲侧特征（白色体色和对侧眼的移位），并且对甲状腺激素的组织反应性在身体的左右侧之间存在差异。为了发育成正常的青少年，甲状腺激素必须在一个有限的时间窗口内分泌，当反应只出现在一侧时。本研究的目的是更详细地证实这一假说的有效性和普遍性。对牙鲆发育过程中所有色素细胞的活体外观进行了研究，发现存在以下三种类型的外观庄园：1）对称性幼虫型（幼虫型黑色素细胞、黄色素细胞和白色素细胞），2）不对称成虫型（3）对称性成虫型（虹膜细胞）。为进一步研究，我们尝试了体外培养色素细胞的方法。在本项目期末，我们已经建立了所有色素细胞（除幼虫黄色素细胞外）出现的培养条件。在日本的比目鱼中，黏比目鱼（Microstomus achne）具有非常长的浮游生活阶段，而星比目鱼（Platichthys stellatus）具有相对较短的浮游生活阶段。对于这两个物种，甲状腺激素（T4，甲状腺素）被添加到饲养罐在不同的时间，和正常的，pseudoalbino，和ambicolorate的组成，完成变态后进行了检查。定时T4除了显着改变的比例，与庄园，可以解释的时间假说。此外，正常的青少年的比例最高，观察到在一个类似的时间，以海洋的幼虫变态的坦克。这些结果表明，这两个物种的假设的一般性。

项目成果

変態期の左右性決定への甲状腺ホルモンの関与

甲状腺激素参与变态过程中侧向性的确定

• 发表时间: 2005
• 期刊: 日本水産学会誌 71
• 作者: Sergio Bolasina;Masatomo Tagawa;Yoh Yamashita;Masaru Tanaka;Nozomi Okada;Masatomo Tagawa;田川正朋
• 通讯作者: 田川正朋

ババガレイの変態異常に及ぼす甲状腺ホルモン投与時期の影響

甲状腺激素给药时机对比目鱼异常变态的影响

• 发表时间: 2007
• 作者: Tagawa;M;田川正朋
• 通讯作者: 田川正朋

ヒラメの変態期には質的に異なる2型(仔魚型・稚魚型)の黄色素胞が存在する

在牙鲆的变态阶段，有两种性质不同的黄色细胞（幼体型和幼年型）。

• 发表时间: 2007
• 作者: Tagawa;M;田川正朋;西川泰造;中村光男
• 通讯作者: 中村光男

IIIA.甲状腺ホルモン(P.1908-1929) (ホルモンハンドブック新訂eBook版、日本比較内分泌学会編)

IIIA.甲状腺激素（P.1908-1929）（激素手册新修订电子书版，日本比较内分泌学会编）

• 发表时间: 2007
• 作者: Aritaki;M;田川正朋;田川正朋
• 通讯作者: 田川正朋

Effect of stocking density on growth, digestive enzyme activity and cortisol level in larvae and juveniles of Japanese flounder, Paralichthys olivaceus

• DOI: 10.1016/j.aquaculture.2006.05.021
• 发表时间: 2006-09-08
• 期刊: AQUACULTURE
• 影响因子: 4.5
• 作者: Bolasina, Sergio;Tagawa, Masatomo;Tanaka, Masaru
• 通讯作者: Tanaka, Masaru