Cholinergic receptor muscarinic 3 (CHRM3) and 63-adrenoceptor (B_3 AR) polymorphisms in irritable bowel syndrome

胆碱能受体M受体3(ChRM3)和β3肾上腺素能受体(β3 AR)基因多态性与肠易激综合征的相关性已得到证实。我们检测了CHRM3基因(Rs3738435)和β_3AR基因(Trp64Arg)多态性之间的关系。从82例IBS患者(腹泻型32例，便秘型45例，未分型5例)和68例正常对照中提取DNA。用直接测序法测定基因的多态。CHRM3基因多态性在IBS组和对照组之间无显著差异。相比之下，肠易激综合征患者β_3AR(T/C+C/C)基因多态性的发生率显著高于对照组(P&lt；0.05)。此外，便秘型肠易激综合征患者β_3AR(T/C+C/C)基因多态频率明显高于腹泻型肠易激综合征患者。因此，β_3AR(Trp64Arg)基因多态性与肠易激综合征，尤其是便秘型肠易激综合征密切相关。

The association between polymorphisms in the cholinergic receptor muscarinic 3 (CHRM3) and β3-adrenoceptor (β3 AR) genes in irritable bowel syndrome (IBS) has been well established. We examined the association between the CHRM3 (rs3738435) and β_3 AR gene (Trp64Arg) polymorphisms. DNA was obtained from 82 IBS patients (32 diarrhea type, 45 constipation type, 5 unclassified) and 68 controls. Polymorphisms were determined by direct sequencing. No significant differences in CHRM3 polymorphisms were observed between IBS and controls. In contrast, β_3 AR (T/C + C/C) polymorphisms occurred at a significantly higher rate in IBS patients compared to controls (P<0.05). Furthermore, the rate of β_3 AR (T/C + C/C) polymorphisms in constipation-predominant IBS was significantly higher than that in diarrhea-predominant IBS. Thus, β_3 AR (Trp64Arg) polymorphisms appear to be strongly associated with IBS, particularly constipation-predominant IBS.