Study ofion channels involved in uterine smooth muscle cells contraction by using molecular physiological approach

分子生理学方法研究参与子宫平滑肌细胞收缩的离子通道

• 批准号: 17591714
• 负责人: TAKAHASHI Toshifumi
• 金额: $ 2.41万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591714/
• 关键词: Ion channels; Smooth muscle cells; Pregnancy; Calcium channel; Potasium channel; 分子生理学

This is the last year of this study. So we analyzed the results taken from the experiments described below. First, we performed the experiment of expression of ion channels in pregnant smooth muscle cells; Expression of calcium channels, such as inositol tri-phosphate (IP3) receptors and ryanodine receptors; Expression of sodium and potassium channels. Expression of calcium channels, such as IN and ryanodine receptors in rat myometrium were increased with increasing gestational age. Second, we performed the experiment of the effects of oxytocin and beta-stimulant on pregnant myometrium by using electrophysiological approach. Third, we performed the experiment of the effects of oxytocin and beta-stimulants on intracellular calcium concentrations in pregnant myometrium. The intracellular calcium concentration in pregnant myometrium was increased dose-dependently by treatment of oxytocin. The increased calcium concentration induced by oxytocin was inhibited by treatment of beta-stimulants. These results suggest that the expression and function of various ion channels in smooth muscle cells are modulated by gestational age.

这是这项研究的最后一年。因此，我们分析了下面描述的实验结果。首先，我们进行了妊娠平滑肌细胞中离子通道表达的实验;钙通道的表达，如三磷酸肌醇（IP 3）受体和兰尼碱受体;钠和钾通道的表达。随着胎龄的增加，大鼠子宫肌层钙通道如IN和ryanodine受体的表达增加。第二，采用电生理方法研究催产素和β-兴奋剂对妊娠子宫肌层的影响。第三，我们进行了催产素和β-兴奋剂对妊娠子宫肌细胞内钙浓度的影响的实验。子宫肌细胞内钙离子浓度在催产素处理后呈剂量依赖性增加。催产素诱导的钙浓度增加被β-兴奋剂抑制。这些结果表明，平滑肌细胞中各种离子通道的表达和功能受胎龄的调节。