Analysis of inner ear specific autoantibody in experimental autoimmune labyrinthitis model

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In order to establish experimental inner ear specific autoimmune labyrinthitis in mice, we designed to recover inner ear specific proteins from crude inner ear proteins by SDS-technique and proteome profiling. Thirty partitioned inner ear proteins according to molecular size were harvested through elution of crude inner ear antigens on Whole Gel Eluter. Serum from mice sensitized with 19 out of 30 partitions produced antibodies against bovine inner ear proteins by western blotting and 7 positive different bands were recognized. Proteome profiling of several partitions that demonstrated clear positive bands were selectively carried out. Partition number 3, 10 and 11 hit only COCH protein. Partition number 18, 22 and 24 hit non specific inner ear proteins. This study succeeded in isolation of inner ear specific proteins, suggesting that development of inner ear specific autoimmune animal model is possible to elucidate pathogenesis of autoimmune inner ear disease.