Analysis of inner ear specific autoantibody in experimental autoimmune labyrinthitis model

实验性自身免疫性迷路炎模型内耳特异性自身抗体分析

• 批准号: 17591803
• 负责人: TOMIYAMA Shunichi
• 金额: $ 2.24万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591803/
• 关键词: inner ear autoimmune disease; inner ear specific autoantibody; animal model; SDS-PAGE; whole gel eluter; western blotting; proteome profiling; inner ear specific auto-antigen; 自己免疫病; 電気泳動; ウェスタンブロット

In order to establish experimental inner ear specific autoimmune labyrinthitis in mice, we designed to recover inner ear specific proteins from crude inner ear proteins by SDS-technique and proteome profiling. Thirty partitioned inner ear proteins according to molecular size were harvested through elution of crude inner ear antigens on Whole Gel Eluter. Serum from mice sensitized with 19 out of 30 partitions produced antibodies against bovine inner ear proteins by western blotting and 7 positive different bands were recognized. Proteome profiling of several partitions that demonstrated clear positive bands were selectively carried out. Partition number 3, 10 and 11 hit only COCH protein. Partition number 18, 22 and 24 hit non specific inner ear proteins. This study succeeded in isolation of inner ear specific proteins, suggesting that development of inner ear specific autoimmune animal model is possible to elucidate pathogenesis of autoimmune inner ear disease.

为了建立小鼠实验性内耳特异性自身免疫性中耳炎模型，我们采用SDS-聚丙烯酰胺凝胶电泳和蛋白质组分析技术从内耳蛋白中提取内耳特异性蛋白。通过在全凝胶洗脱器上洗脱粗制内耳抗原，获得30种根据分子大小划分的内耳蛋白。免疫印迹法检测30个分离物中有19个分离物致敏小鼠血清产生抗牛内耳蛋白抗体，共识别出7条阳性条带。选择性地进行显示清晰阳性条带的几个分区的蛋白质组分析。分区号3、10和11仅命中COCH蛋白。分区号18、22和24命中非特异性内耳蛋白。本研究成功分离了内耳特异性蛋白，提示建立内耳特异性自身免疫动物模型，为阐明自身免疫性内耳病的发病机制提供了可能。

项目成果

Clinical experience of Autoimmune inner ear disease

自身免疫性内耳疾病的临床经验

• 发表时间: 2007
• 作者: Shunichi;Tomiyama;Akihiko;Saito;Nozomu;Ogiwara;Chika;Ozu;Manabu;Nonaka;Mayumi;Kunitomo
• 通讯作者: Kunitomo

プロテオーム解析に基づく内耳特異的蛋白抗原の分離回収

基于蛋白质组分析的内耳特异性蛋白抗原的分离和回收

• 发表时间: 2008
• 期刊: Otol Jpn 18
• 作者: 伊藤壽一;中川隆之;富山 俊一
• 通讯作者: 富山 俊一

Expression of cochlin in the vestibular organ of rats

• DOI: 10.1159/000089404
• 发表时间: 2005-01-01
• 期刊: ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY AND ITS RELATED SPECIALTIES
• 作者: Ikezono, T;Shindo, S;Yagi, T
• 通讯作者: Yagi, T

Immunological pathogenesis in vertigo attack

眩晕发作的免疫学发病机制

• 发表时间: 2008
• 期刊: Equilibrium Res 67-3
• 作者: Shunichi;Tomiyama
• 通讯作者: Tomiyama

A case of low tone sensory nerve hearing loss with positive inner ear autoantiboclies

内耳自身抗体阳性的低音感觉神经性听力损失一例

• 发表时间: 2007
• 期刊: Otol Jpn 17-1
• 作者: Shunichi;Tomiyama;Akihiko;Saito;Manbu;Nonaka
• 通讯作者: Nonaka