The effect of Glicentin on Bacterial Translocation

肠高血糖素对细菌易位的影响

• 批准号: 17591869
• 负责人: CHIBA Masahiro
• 金额: $ 2.39万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591869/
• 关键词: enterogulucagon; glicentin; Bacterial Translocation; mucosal barricr; rat; MMC; TPN; GLP-2; sIgA; エンドトキシン

Aim : Glicentin, the main component of enteroglucagon, has trophic effects on intestinal mucosa. It may also have an inhibitory effect on extraintestinal invasion of enteric bacteria. But, recent studies have failed to show the effect on the small intestinal motility. This study evaluated the effects of recombinant human glicentin on the physiological fasted motor activities of freely moving conscious rats with force transducers implanted in the duodenum and jejunum under total parenteral nutrition (TPN).Method : Male wistar rats weighing 200-250 grams were used. Male wistar rats were equipped with force transducers on the stomach, duodenum, and proximal jejunum. All experiments were performed after 8 hours of fasting. The small intestinal transit rate was measured after the administration of glicentin or/and GLP-2. Contractile motor activity of the bowel in the interdigestive state was also measured following the administration of these drugs.Results : In the initial experiment, MMC was dose-dependently reduced by glicentin and GLP-2, when compared with the control. In the second experiment, glicentin did not reveal synergistic effects in combination with GLP-2.Conclusion : Administration of glicentin may be useful to enhance the stagnation time in small intestine. This was thought to be an action of the growth factor in the intestines to the proliferation of the gut mucosa and the reinforcement of the barrier of both immunological and mechanical function as paracrain.

目的：肠胰高血糖素的主要成分甘肽素对肠黏膜具有营养作用。它也可能对肠道细菌的肠外入侵有抑制作用。但是，最近的研究未能显示其对小肠运动的影响。在全肠外营养（TPN）下，研究了重组人格立甘肽对在十二指肠和空肠植入力传感器的自由运动意识大鼠生理禁食运动活动的影响。方法：选用雄性wistar大鼠，体重200 ~ 250 g。在雄性wistar大鼠的胃、十二指肠和近空肠上安装了力传感器。所有实验均在禁食8小时后进行。在给药格列汀或/和GLP-2后测定小肠转运率。在服用这些药物后，还测量了消化间期肠道的收缩运动活动。结果：在初始实验中，与对照组相比，glicentin和GLP-2使MMC呈剂量依赖性降低。在第二个实验中，glicentin与GLP-2合用未显示出协同作用。结论：格列汀可有效延长小肠滞留时间。这被认为是肠内生长因子对肠黏膜增殖的作用，增强了免疫和机械功能的屏障。

项目成果

Glicentin Inhibits Internalization of Enteric Bacteria in Cultured INT-407 Enterocytes

高血糖素抑制培养的 INT-407 肠细胞中​​肠道细菌的内化

• 发表时间: 2007
• 期刊: Pediatr Surg Int 23(6)
• 作者: Masahiro Chiba;Yutaka Sanada;Yasuo Yoshizawa;et. al.
• 通讯作者: et. al.