Microarray and proteomics analysis on odontogenesis-related-genes in prenatal mouse dental papillae

据报道，在小鼠牙乳头发育的前后阶段，参与牙形成的重要因素消失了。因此，我们推测，在出生后，参与牙乳头成牙过程的基因可能会下调。我们的目标是通过微阵列分析确定哪些基因是下调基因。从16日龄、18日龄（E16、E18）和出生后3日龄、10日龄（P3、P10）小鼠第一下颌磨牙胚中分离分离牙乳头。E16和E18下调Adamts4和Aldhala2基因，Plxncl基因在E18和P3下调表达。定量RT-PCR检测了Adamts4、Aldhla2和Plxncl的表达，结果显示出生后它们的表达减少（Adamts4; 1/3, Aldhla2; 1/13, Plxncl; 1/3）。这些结果表明，通过微阵列分析选择的三个基因- Adamts4， Aldhla2和Plxncl -参与了牙乳头的牙形成。

Important factors for involved in odontogenesis in mouse dental papillae have been reported to disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that the genes involved in odontogenesis in dental papillae might be down-regulated after birth. Our goal was to identify those which genes were down-regulated genes by microarray analysis. Dental papillae were dissected isolated from embryonic of 16-day and 18-day (E16, E18), and post-natal 3- and 10-day-old (P3, P10) murine mice first mandibular molar germs. The highest number of down-regulated genes (2924) was found at E18 and P3 Down-regulation of Adamts4 and Aldhala2 was found at both E16 and E18, and expression of Plxncl showed a reduction at E18 and P3. Investigation of expression of Adamts4, Aldhla2 and Plxncl was investigated by quantitative RT-PCR which showed revealed a reduction of in their expression after birth (Adamts4; 1/3, Aldhla2; 1/13, Plxncl; 1/3). These results suggest that three of the genes selected identified by the microarray analysis - Adamts4, Aldhla2, and Plxncl - are involved in odontogenesisin dental papillae.