Microarray and proteomics analysis on odontogenesis-related-genes in prenatal mouse dental papillae

产前小鼠牙乳头成牙相关基因的微阵列和蛋白质组学分析

• 批准号: 17591926
• 负责人: MURAMATSU Takashi
• 金额: $ 2.21万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591926/
• 关键词: odontogenesis; tooth germ; dental ID anilla; microarrav; proteomics; Admts4; Aldh1a2; PlexinC1; in situ hvbridization; Aldh1a2; 歯牙形成能

Important factors for involved in odontogenesis in mouse dental papillae have been reported to disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that the genes involved in odontogenesis in dental papillae might be down-regulated after birth. Our goal was to identify those which genes were down-regulated genes by microarray analysis. Dental papillae were dissected isolated from embryonic of 16-day and 18-day (E16, E18), and post-natal 3- and 10-day-old (P3, P10) murine mice first mandibular molar germs. The highest number of down-regulated genes (2924) was found at E18 and P3 Down-regulation of Adamts4 and Aldhala2 was found at both E16 and E18, and expression of Plxncl showed a reduction at E18 and P3. Investigation of expression of Adamts4, Aldhla2 and Plxncl was investigated by quantitative RT-PCR which showed revealed a reduction of in their expression after birth (Adamts4; 1/3, Aldhla2; 1/13, Plxncl; 1/3). These results suggest that three of the genes selected identified by the microarray analysis - Adamts4, Aldhla2, and Plxncl - are involved in odontogenesisin dental papillae.

据报道，在发育前和产后阶段之间，涉及小鼠牙皮乳头的牙肠发生的重要因素。因此，我们假设牙齿乳头状的牙肠发生的基因可能会在出生后下调。我们的目标是通过微阵列分析鉴定基因下调基因的基因。从16天和18天（E16，E18）的胚胎中分离出牙皮乳头，以及3天和10天大的幼虫（P3，P10）鼠小鼠首次下颌摩尔细菌。在E18时发现了最多的下调基因（2924），并且在E16和E18上都发现了ADAMTS4和ALDHALA2的P3下调，PLXNCL的表达在E18和P3时显示出降低。通过定量RT-PCR研究了ADAMTS4，Aldhla2和PLXNCL的表达的研究，该定量RT-PCR显示出出生后其表达的降低（Adamts4; 1/3; 1/3，Aldhla2; 1/13; 1/13，plxncl; 1/3）。这些结果表明，通过微阵列分析鉴定的三个基因 - ADAMTS4，Aldhla2和PLXNCL - 参与牙乳状核酸酯的牙胎。

项目成果

The epithelial-mesenchymal interaction for tooth morphogenes is with special reference to the arrest of normal tooth development in the diastema budding

牙齿形态发生的上皮-间质相互作用特别涉及纵裂出芽时正常牙齿发育的停滞

• 发表时间: 2006
• 期刊: Research Trends Current Topics in Genetics 2
• 作者: Hitoshi Yamamoto;Sung-Won Cho;Jae-Young Kim;Han-Sung Jung
• 通讯作者: Han-Sung Jung

Gene Chipマイクロアレイによるマウス臼歯の歯乳頭、歯髄の遺伝子発現の比較

使用基因芯片微阵列比较小鼠磨牙牙乳头和牙髓中的基因表达

• 发表时间: 2005
• 作者: 佐々木 穂高;村松 敬;太田 一正;君塚 隆太;橋本 貞充;下野 正基
• 通讯作者: 下野 正基

Immunohistochemical localization for manganese and copper, zinc superoxide dismutase in secretory ameloblasts of rat incisor

大鼠门牙分泌性成釉细胞中锰、铜、锌超氧化物歧化酶的免疫组织化学定位

• 发表时间: 2005
• 期刊: Journal of Comparative Development of Tooth Enamel 9
• 作者: Hitoshi;Yamamoto;Rumi;Yokota;Kunihiro;Suzuki;Yuka;Iwasa;Hideki;Chisaka;Yukishige;Kozawa
• 通讯作者: Kozawa

Ca^<2+> transporting/signaling mechanisms mediated by sodium/calcium exchangers in rat ameloblasts

大鼠成釉细胞中钠/钙交换剂介导的 Ca^2 转运/信号传导机制

• 发表时间: 2007
• 作者: Okumura R;Suzuki K;Muramatsu T;Nakagawa K;Shimono M;Schnetkamp P PM;Shibukawa Y.
• 通讯作者: Shibukawa Y.

歯性間葉組織がもつ歯の潜在的形成能について

关于牙源性间充质组织潜在的牙齿形成

• 发表时间: 2006
• 作者: 山本 仁;脇坂 聡;土門 卓文;斎藤 正寛;今井 元;Herve Lesot;小澤 幸重
• 通讯作者: 小澤 幸重