Microarray and proteomics analysis on odontogenesis-related-genes in prenatal mouse dental papillae

据报道，参与小鼠牙乳头发育的重要因素在出生前和出生后的发育阶段之间消失。因此，我们推测，在牙乳头中参与牙发生的基因可能在出生后下调。我们的目标是通过微阵列分析确定哪些基因是下调基因。本实验从16、18日龄（E16、E18）和出生后3、10日龄（P3、P10）的小鼠下颌第一磨牙胚中分离牙乳头。在E18和P3发现最高数量的下调基因（2924）。在E16和E18发现Adamts 4和Aldhala 2的下调，并且Plxncl的表达在E18和P3显示降低。通过定量RT-PCR研究Adamts 4、Aldhla 2和Plxncl的表达，其显示出生后其表达降低（Adamts 4; 1/3，Aldhla 2; 1/13，Plxncl; 1/3）。这些结果表明，通过微阵列分析确定的三个基因-Adamts 4，Aldhla 2和Plxncl -参与牙乳头中的牙发生。

Important factors for involved in odontogenesis in mouse dental papillae have been reported to disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that the genes involved in odontogenesis in dental papillae might be down-regulated after birth. Our goal was to identify those which genes were down-regulated genes by microarray analysis. Dental papillae were dissected isolated from embryonic of 16-day and 18-day (E16, E18), and post-natal 3- and 10-day-old (P3, P10) murine mice first mandibular molar germs. The highest number of down-regulated genes (2924) was found at E18 and P3 Down-regulation of Adamts4 and Aldhala2 was found at both E16 and E18, and expression of Plxncl showed a reduction at E18 and P3. Investigation of expression of Adamts4, Aldhla2 and Plxncl was investigated by quantitative RT-PCR which showed revealed a reduction of in their expression after birth (Adamts4; 1/3, Aldhla2; 1/13, Plxncl; 1/3). These results suggest that three of the genes selected identified by the microarray analysis - Adamts4, Aldhla2, and Plxncl - are involved in odontogenesisin dental papillae.