Analysis of biological function, specificity and interactions of cellulose synthases

纤维素合酶的生物学功能、特异性和相互作用分析

• 批准号: 5351631
• 负责人: Professor Dr. Wolf-Rüdiger Scheible
• 金额: --
• 依托单位: Samuel Roberts Noble Foundation
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5351631?language=en
• 关键词: Analysis; biological; function; specificity; interactions

The family of cellulose synthases (CesA) genes is composed of ten members in Arabidopsis. Mutants in the five more highly expressed CesA genes have been identified and recently cloned, based on strong and characteristic cell wall / cell expansion phenotypes, and hence some degree of biological function has been assigned to those genes. In contrast, no biological function or specific expression data are available for the other five, more lowly expressed, family members, yet. This could suggest that they either encode tissue-specific isoforms or are expressed only during specific stages of development or under special circumstances, and corresponding mutants might display no or just subtle phenotypes under normal conditions. The goals of this proposal are to generate the missing information for these additional genes - by studying the morphology and development of the corresponding T-DNA insertion mutants, as well as their polysaccharide composition, and -by analyzing the expression pattern and level of the corresponding CesA transcripts and -proteins in a large range of conditions. Another goal is to examine which of the CesA isoforms are required in parallel in a cellulose-synthesizing complex in a specific cell, and what other interacting (regulatory?) proteins are required in order to allow for normal cellulose production.

拟南芥中的纤维素酶（CesA）基因家族由10个成员组成。基于强的和特征性的细胞壁/细胞扩增表型，已经鉴定并最近克隆了五种更高表达的CesA基因中的突变体，因此已经将一定程度的生物学功能分配给这些基因。相比之下，没有生物学功能或特定的表达数据可用于其他五个更低表达的家族成员。这可能表明，它们要么编码组织特异性亚型，要么仅在发育的特定阶段或特殊情况下表达，相应的突变体在正常条件下可能不显示或仅显示微小的表型。该提案的目标是通过研究相应的T-DNA插入突变体的形态和发育以及它们的多糖组成，以及通过分析相应的CesA转录本和蛋白质在大范围条件下的表达模式和水平，来产生这些额外基因的缺失信息。另一个目标是检查在特定细胞中的纤维素合成复合物中并行需要哪些CesA同种型，以及其他什么相互作用（调节？）需要蛋白质以允许正常的纤维素生产。