Total DNA sequencing of mitochondrial genome on Japanese Laminaria species for analysls of their phylogeny and speciation

日本海带物种线粒体基因组的全 DNA 测序，用于分析其系统发育和物种形成

• 批准号: 18570077
• 负责人: YOTSUKURA Norishige
• 金额: $ 2.28万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570077/
• 关键词: seaweeds; Japanese kelp; genetic diversity; phylogeny; speciation; mitochondrial DNA; sequencing

The mtDNA of seven species in this study were showed as circular mapping organization. They contain genes for three rn, for twenty-five trn, for ten nad, for one cob, for three cox, for three atp, for one tat and for three ORF as coding regions in the same way. The organization is identical with that of L. digitata except for nonexistence of ORF 157. The nucleotide length of mtDNA was 37,657bp for L. japonica (Lj), L. religiosa (Lr), L. diabolica (Ld), L. longipedalis (Ll) and was 37,656bp for L. ochotensis. While on the other hand, it was 37,604bp and 37,500bp for L. angustata (La) and L. coriacea (Lc) respectively.As a result of nucleotide alignment, though there was no nucleotide insertion / deletion through total mtDNA among Li, Lr, Ld and Ll, there was one nucleotide insertion / deletion at one of rps 19 between them and Lo. Nucleotide differences are poor among these five species in sum total and pairwise distance (nad6 (partial)-co x1) (: PD partial) between them are 0.02-0.11 through total mtDNA. Nucleotide substitutions and nucleotide insertions / deletions were detected at 69 sites from their package nucleotide alignment, and the value of nucleotide substitution rate was high especially at trnL, trnM, rps19, ORF41 and seven spacer. On the contrary, nucleotide differences were abundant between the five species and the other two species, and PD partial between the five species and La was 4.36-5.85. And nucleotide maxim matching between Li, La and Lc was 95-96%.From this study, genetic differences between Japanese Laminaria species were detected as sequence differences at multi-regions on mitochondrial genome. Within them, several seem to be useful tools for taxonomic and phylogenetic studies. Furthermore, phylogenetic trees for seven species were constructed, and short history for speciation within Lr, Lo, Ld and Ll was presumed.

本研究的7个物种的mtDNA呈环状作图组织。它们包含3个rn， 25个trn， 10个nad， 1个cob， 3个cox， 3个atp， 1个tat和3个ORF作为编码区，方式相同。除了不存在orf157外，组织结构与L. digitata相同。L. japonica （Lj）、L. religiosa （Lr）、L. diabolica （Ld）、L. longipedalis （Ll）的mtDNA核苷酸长度为37,657bp， L. ochotensis的mtDNA核苷酸长度为37,656bp。L. angustata （La）和L. coriacea （Lc）分别为37,604bp和37,500bp。由于核苷酸比对，虽然Li、Lr、Ld和Ll之间的总mtDNA中没有核苷酸插入/缺失，但它们与Lo之间的rps 19中有一个核苷酸插入/缺失。这5个物种的总核苷酸差异不大，通过总mtDNA，它们之间的两两距离（nad6（偏）-co x1） （: PD偏）为0.02 ~ 0.11。在其包体核苷酸比对中发现了69个位点的核苷酸替换和核苷酸插入/缺失，其中trnL、trnM、rps19、ORF41和7个间隔段的核苷酸替换率较高。相反，5种与其他2种之间的核苷酸差异较大，5种与La之间的PD偏值为4.36 ~ 5.85。Li、La和Lc的核苷酸最大匹配度为95 ~ 96%。本研究发现日本海带物种间的遗传差异表现为线粒体基因组多区域序列差异。其中有几个似乎是分类学和系统发育研究的有用工具。此外，构建了7个物种的系统发育树，推测Lr、Lo、Ld和Ll的物种形成历史较短。

项目成果

The morphological diversity and the genetic variation of Ecklonia cava and E. kurome (Laminariales, Phaeophyta) sprophytes collected along the coasts of Wakayama prefecture

和歌山县沿海采集的 Ecklonia cava 和 E. kurome (Laminariales, Phaeophyta) 孢子体的形态多样性和遗传变异

• 发表时间: 2007
• 期刊: Aquaculture Science 55
• 作者: Shimoda M;Insect Clock Genes;Sekine T;Shimoda M;Adachi-Yamada T;Shimoda M;Adachi-Yamada T;Ishida N;Nishinokubi I;Adachi-Yamada T;Ishida N;入江美代子;Irie-Kubo M;Sekine T;関根立守;Sekine T;Sekine T;関根 立守;Shimoda M;Shimoda M;Mohri H;関根立守;Mohri H;Sekine T;霜田政美(分担執筆192-193ページ);川井唯史;Norishige Yotsukura;Tadashi Kawai;Norishige Yotsukura;Norishige Yotsukura;田中俊充;江端弘樹;Kouhei Nagai;四ツ倉典滋;Toshimitsu Tanaka
• 通讯作者: Toshimitsu Tanaka

A review on nomenclature of laminariacean kelp growing in cold water areas of Japan

日本冷水区海带类海带的命名研究进展

• 发表时间: 2007
• 期刊: Japanese Journal of Phycology 55
• 作者: Shimoda M;Insect Clock Genes;Sekine T;Shimoda M;Adachi-Yamada T;Shimoda M;Adachi-Yamada T;Ishida N;Nishinokubi I;Adachi-Yamada T;Ishida N;入江美代子;Irie-Kubo M;Sekine T;関根立守;Sekine T;Sekine T;関根 立守;Shimoda M;Shimoda M;Mohri H;関根立守;Mohri H;Sekine T;霜田政美(分担執筆192-193ページ);川井唯史;Norishige Yotsukura;Tadashi Kawai;Norishige Yotsukura;Norishige Yotsukura;田中俊充;江端弘樹;Kouhei Nagai;四ツ倉典滋;Toshimitsu Tanaka;Hiroki Ebata;Kouhei Nagai;Norishige Yotsukura
• 通讯作者: Norishige Yotsukura

Protein extraction for two-dimensional electrophoresis from the lamina of Ecklonia kurome(Laminariales), a recalcitrant tissue containing hight levels of viscous polysaccharides

从 Ecklonia kurome（昆布目）的叶片（一种含有高水平粘性多糖的顽固组织）中提取蛋白质并进行二维电泳

• 发表时间: 2007
• 期刊: Electrophoresis 29
• 作者: Shimoda M;Insect Clock Genes;Sekine T;Shimoda M;Adachi-Yamada T;Shimoda M;Adachi-Yamada T;Ishida N;Nishinokubi I;Adachi-Yamada T;Ishida N;入江美代子;Irie-Kubo M;Sekine T;関根立守;Sekine T;Sekine T;関根 立守;Shimoda M;Shimoda M;Mohri H;関根立守;Mohri H;Sekine T;霜田政美(分担執筆192-193ページ);川井唯史;Norishige Yotsukura;Tadashi Kawai;Norishige Yotsukura;Norishige Yotsukura;田中俊充;江端弘樹;Kouhei Nagai;四ツ倉典滋;Toshimitsu Tanaka;Hiroki Ebata;Kouhei Nagai;Norishige Yotsukura;Satoshi Shimada;Kouhei Nagai
• 通讯作者: Kouhei Nagai

コンブ目海藻カジメのタンパク質発現に与える水温の影響

水温对层状海藻 Kajime 蛋白表达的影响

• 发表时间: 2007
• 作者: 永井宏平;Kouhei Nagai;永井宏平
• 通讯作者: 永井宏平

Formation of aldehyde flavor (n-hexanal, 3Z-nonenal and 2E-nonenal) in the brown alga, Laminaria angustata

• DOI: 10.1007/s10811-006-9038-6
• 发表时间: 2006-10-01
• 期刊: JOURNAL OF APPLIED PHYCOLOGY
• 影响因子: 3.3
• 作者: Boonprab, Kangsadan;Matsui, Kenji;Kajiwara, Tadahiko
• 通讯作者: Kajiwara, Tadahiko