Elucidation of novel cAMP signal transductions in myxobacteria

阐明粘细菌中新型 cAMP 信号转导

• 批准号: 18570131
• 负责人: KIMURA Yoshio
• 金额: $ 2.66万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570131/
• 关键词: PKA; セリン; スレオニンプロテインキナーゼ; cAMP; シグナル伝達; 細菌

粘液細菌よりcAMP依存性プロテインキナーゼ(PKA)の検索を試みた結果、PKAの特異的基質を最もよい基質とし, PKAの特異的阻害剤によって活性阻害を受けるSpkAキナーゼを得たが、真核生物のPKAで見られるcAMPと調節サブユニットによる酵素活性調節は見られなかった。また、本菌のcAMP分解酵素は、その変異株より、胞子の発芽時の浸透圧ストレス応答に関与していると推定し、また、酵素学的諸性質の検討から、基質特異性の広い新規性の高い酵素であった。

The results of detection of cAMP-dependent protein kinase A (PKA) in myxobacteria showed that PKA specific matrix was the most important matrix, PKA specific inhibitor was the most active inhibitor, and PKA was found to regulate cAMP activity in eukaryotes. In addition, the cAMP decomposing enzyme of this bacterium is related to the estimation of the permeability of the cell during germination, the study of the properties of enzymology, and the identification of novel enzymes with substrate specificity.

项目成果

Contribution of cyclic nucleotide phosphodiesterases, PdeA and PdeB, in adaptation of Myxococcus xanthus cells to osmotic or high temperature stress.

环核苷酸磷酸二酯酶 PdeA 和 PdeB 在黄粘球菌细胞适应渗透或高温应激中的贡献。

• 发表时间: 2006
• 期刊: Journal of Bacteriology 188
• 作者: Kimura;Y.;Nakatuma;H;Sato;N.;and Ohtani;M.;Y.Kimura
• 通讯作者: Y.Kimura

Enzymatic characteristics of two novel Myxococcus xanthus enzymes, PdeA and PdeB, displaying 3′,5′- and 2′,3′-cAMP phosphodiesterase, and phosphatase activities

• DOI: 10.1016/j.febslet.2008.12.044
• 发表时间: 2009-01-22
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Kimura, Yoshio;Okazaki, Nozomi;Takegawa, Kaoru
• 通讯作者: Takegawa, Kaoru

Enzymatic characteristics of a Ser/Thr protein kinase, SpkA, from Myxococcus xanthus

来自黄色粘球菌的 Ser/Thr 蛋白激酶 SpkA 的酶学特征

• 发表时间: 2009
• 期刊: Journal of Biosci-ence and Bioengineering 107
• 作者: Kimura;Y.;Kakemizu;A. Matsubara;Y.;and Takegawa;K
• 通讯作者: K

Enzymatic characteristics of two novel Myxococcus xanthus enzymes

两种新型黄色粘球菌酶的酶学特征

• 发表时间: 2008
• 期刊: PdeA and PdeB, displaying 3', 5'- and 2', 3'-cAMP phospho- diesterase, and phosphatase activities FEBS Letters 583
• 作者: Kimura;Y.;Okazaki;N.;and Takegawa;K
• 通讯作者: K

Anal-ysis of functional properties of PirA and ScdA in Myxococcus xanthus

黄色粘球菌PirA和ScdA的功能特性分析

• 发表时间: 2007
• 期刊: Technical bulletin of faculty of agriculture, Kagawa University 59
• 作者: Kimura;Y.;and Hamanaka;H.
• 通讯作者: H.