权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular mechanism for formation of two types of flowers in Hydrangea

绣球花两种花形成的分子机制

基本信息

批准号：
18580031
负责人：
UEMACHI Tatsuya
金额：
$ 2.51万
依托单位：
The University of Shiga Prefecture
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580031/
关键词：
Decorative flower Petaloidy Leafy Apetala 3 Retrotransposon Bud sport Hydrangea Dimorphic flower APETALA1 PISTILLATA SSH法レトロトランソポゾン

项目摘要

The purpose of this study is the elucidation of the molecular mechanism for formation of two types of flowers in HydrangeaThree AP1, two A.11 one PIhomologues were isolated from H. macrophylla BM-1'. RT-PCR analysis for expression of several genes including these homologues showed LFYhomologue was expressed specifically in petaloid sepals of the decorative flowers. On the other hand, in situ hybridization analysis showed HmAP3, one of the two AP3homologues, was not expressed in the petaloid sepals.The suppression subtractive hybridization (SSH) method was used to isolate differentially expressed genes on the flower buds between the lacecap cultivar Blue Sky' and the hortensia cultivar BM-l' which was bud sports from Blue Sky'. As show by RT-PCR analysis for expression of sixteen kinds of cDNA clones from Blue Sky' SSH library which did not show similarity to any genes on BLAST search, these clones were expressed on flower buds of Blue Sky' more strongly than on those of 'BM-1'. These clones were classified into four groups according to the expression pattern on flower buds, leaf buds and leaves of Blue Sky' and 'BM-1'.As shown analysis for sequences of cDNA clones isolated from Blue Sky' SSH library RT sequences of at least one Ty1/copia-like and six Ty/3/gypsy-like retrotransposons were expressed in Blue Sky'. RT-PCR analysis showed retrotransposon-like genes were expressed on flower buds, leaf buds, leaves and roots in the original cultivar Blue Sky'. In the bud sport cultivar BM-1' the expression of retrotransposon-like genes were suppressed on flower buds, although these were expressed on leaf buds, leaves and roots.

从八仙花BM-1‘中分离到三个AP1，两个A.11和一个PI同源物。对包括这些同源物在内的几个基因的RT-PCR分析表明，LFY同源物在观赏花的瓣状萼片中特异表达。另一方面，原位杂交分析表明，AP3的两个同源物之一的HmAP3在花瓣状隔膜中不表达。利用抑制消减杂交方法分离了紫胶树品种蓝天‘和马蹄草品种BM-L’之间的花蕾差异表达基因。对来自Blue Sky‘SSH文库的16个克隆的表达进行RT-PCR分析，结果表明，这些克隆在Blue Sky’花蕾上的表达强于在‘BM-1’上的表达。根据这些克隆在蓝天‘和’BM-1‘的花蕾、叶芽和叶片上的表达模式，将其分为4组。对从Blue Sky’SSH文库中分离的克隆进行序列分析，发现至少有1个Ty1/Copia样和6个Ty/3/Gypsy样反转录转座子在Blue Sky‘中表达。RT-PCR分析表明，在原品种‘蓝天’的花芽、叶芽、叶片和根上都表达了反转录转座子样基因。在芽运品种BM-1‘中，反转录转座子类似基因的表达在花蕾上受到抑制，但在叶芽、叶和根中表达。