Establishment of a technology to avoid replant failure of asparagus through identification of an allelochemicals by means of female and male somatic embryos

众所周知，芦笋能产生一种化感化学物质，这种化学物质被认为是大田补种失败的因素之一。本研究的目的是建立无菌生物测定系统，以评估芦笋再生物的化感作用，以避免再植失败。得到的重要结果如下：1)将雌性和雄性植物的叶片用于先前建立的夹心法时，我们发现了化感作用。2)在本研究项目中建立的无菌生物测定系统中，我们也发现了由顶端分生组织产生的雌性和雄性再生细胞的化感作用。3)基于上述结果，我们证实了不同品种的化感作用存在差异。4)在无菌生物检测系统中，用芦笋种子代替生菜种子，芦笋幼苗的发育不会受到化感化学物质的干扰。5)在实验期间，LED照射修饰的生物测定体系的化感作用发生了变化。6)被认为用于生物测定系统的缓冲液或凝胶可以包括和浓缩化感化学物质；目标物质的分离和鉴定目前正在进行中。

It is well known that asparagus produce biosynthetically an allelochemical, and the chemical substance have been seemed to be one of the factors for replant failure in the field. The purpose of this study is to establish the aseptic bioassay systems for estimation of allelopathic effects of asparagus regenerants to avoid replant failure. The important results obtained are shown as follows :1) We recognized allelopathic effects when the leaves obtained from female and male plants were used for sandwich method that previously established.2) We also recognized allelopathic effects when the female and male regenerants generated from apical meristems were employed for aseptic bioassay systems that established in this research project.3) Based on the above results, we confirmed the allelopathic effects varied from variety to variety.4) Substituting asparagus seeds for these of lettuce in the case of aseptic bioassay systems, the development of asparagus seedlings has not been disturbed by allelochemical like substances.5) Alteration of allelopathic effects was shown in the modified bioassay systems by irradiation with LED during assay period.6) It is considered that the buffer or gel used for bioassay systems may include and concentrate an allelochemical (s) ; the isolation and identification of target substance (s) are now in progress.