Establishment of a technology to avoid replant failure of asparagus through identification of an allelochemicals by means of female and male somatic embryos

石刁柏体内合成的化感物质是石刁柏移栽失败的原因之一。本研究旨在建立芦笋再生植株化感作用的无菌生物测定体系，以避免再生植株移栽失败。得到的重要结果如下：1）将从雌雄植株获得的叶片用于先前建立的三明治法时，我们确认了化感作用; 2）将从顶端分生组织产生的雌性和雄性再生体用于本研究项目建立的无菌生物测定系统时，我们也确认了化感作用; 3）基于上述结果，我们确认了化感作用在品种之间的差异; 4）在无菌生物测定系统的情况下，芦笋幼苗的发育未受到化感物质的干扰。在改良的生物测定系统中，通过在测定期间用LED照射，显示了化感作用的改变。6）认为用于生物测定系统的缓冲液或凝胶可以包括并浓缩化感物质（s）;目前正在进行目标物质的分离和鉴定。

It is well known that asparagus produce biosynthetically an allelochemical, and the chemical substance have been seemed to be one of the factors for replant failure in the field. The purpose of this study is to establish the aseptic bioassay systems for estimation of allelopathic effects of asparagus regenerants to avoid replant failure. The important results obtained are shown as follows :1) We recognized allelopathic effects when the leaves obtained from female and male plants were used for sandwich method that previously established.2) We also recognized allelopathic effects when the female and male regenerants generated from apical meristems were employed for aseptic bioassay systems that established in this research project.3) Based on the above results, we confirmed the allelopathic effects varied from variety to variety.4) Substituting asparagus seeds for these of lettuce in the case of aseptic bioassay systems, the development of asparagus seedlings has not been disturbed by allelochemical like substances.5) Alteration of allelopathic effects was shown in the modified bioassay systems by irradiation with LED during assay period.6) It is considered that the buffer or gel used for bioassay systems may include and concentrate an allelochemical (s) ; the isolation and identification of target substance (s) are now in progress.