Establishment of a technology to avoid replant failure of asparagus through identification of an allelochemicals by means of female and male somatic embryos

众所周知，芦笋通过生物合成产生一种化感物质，这种化学物质似乎是造成芦笋田间移栽失败的因素之一。本研究的目的是建立芦笋再生体的无菌生物检测体系，以评估芦笋再生体的化感作用，避免移栽失败。所获得的重要结果如下：1)当从雌株和雄株获得的叶片用于先前建立的夹心法时，我们认识到化感作用。2)我们也认识到，当从顶端分生组织产生的雌雄再生植株用于本研究项目建立的无菌生物测定体系时，我们也认识到化感作用。3)基于上述结果，我们证实了化感作用因品种而异。4)在无菌生物测定体系中，用芦笋种子代替生菜种子，芦笋幼苗的发育没有受到类似化感物质的干扰。5)在化感物质的照射下，改良的生物测定系统的化感作用发生了变化。6)认为用于生物测定系统的缓冲液或凝胶可能包含和浓缩一种化感物质(S)；目前正在进行目标物质(S)的分离和鉴定。

It is well known that asparagus produce biosynthetically an allelochemical, and the chemical substance have been seemed to be one of the factors for replant failure in the field. The purpose of this study is to establish the aseptic bioassay systems for estimation of allelopathic effects of asparagus regenerants to avoid replant failure. The important results obtained are shown as follows :1) We recognized allelopathic effects when the leaves obtained from female and male plants were used for sandwich method that previously established.2) We also recognized allelopathic effects when the female and male regenerants generated from apical meristems were employed for aseptic bioassay systems that established in this research project.3) Based on the above results, we confirmed the allelopathic effects varied from variety to variety.4) Substituting asparagus seeds for these of lettuce in the case of aseptic bioassay systems, the development of asparagus seedlings has not been disturbed by allelochemical like substances.5) Alteration of allelopathic effects was shown in the modified bioassay systems by irradiation with LED during assay period.6) It is considered that the buffer or gel used for bioassay systems may include and concentrate an allelochemical (s) ; the isolation and identification of target substance (s) are now in progress.