Molecular characterization of the RNA-binding protein, TRN-1 in the lepidopteran insects
Molecular characterization of the RNA-binding protein, TRN-1 in the lepidopteran insects
批准号:
18580054
负责人:
KOTANI Eiji
金额:
$1.63万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2006
资助国家:
日本
项目状态:
已结题
起止时间:
2006 至 2007
中文摘要
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英文摘要
TRN-1, an RNA-binding protein homologous with mammalian T-cell intracellular antigen-1 has been found to express in every tissue of the Iepidopteran, Bombyx mori and Spodoptera frugiperda. TRN-1 molecule has been shown to possess the domain structure consisting of three-repetitive potential RNA-recognition motifs (RRMs) in the N-terminal followed by auxiliary domain in the C-terminal as similar with TIA-1 family, and to function as a modulator protein for transcripts by investigating the production of foreign recombinant proteins and transcripts in the Bombyx TRN-1 knock-down BmN4 cells (Kotani, et. al., Gene 320, 67-79, 2003). Subcellular localization and role of each domain of the Bombyx TRN-1 in the cultured cells has been investigated by the confocal fluorescence microscopy analyses on the cells over-expressing GFP-TRN-1 fusion protein and GFP-TRN-1 lacking C-terminal auxiliary domain and RRM domain in order to gain more insight into cellular function of the TRN-1. The recombinant GFP-fusion proteins with RRMs were indicated to assuredly have the poly (U) RNA binding activity. It was also shown that GFP-TRN-1 lacking C-terminal auxiliary domain and GFP-C-terminal domain were likely to be localized in the nucleus, while full-length GFP-TRN-1 was localized both in the nucleus and cytoplasm. However, clear localization of GFP-TRN-1 in the nucleus was observed after AcNPV was infected into the cells. In addition, it was indicated that GFP-TRN-1 over-expression could cause change of the recombinant baculovirus-produced luciferase activity during its infection, thus demonstrating that lepidopteran TRN-1 may control the function of both baculovirus and cellular transcripts in the response to stress caused by the viral infection.
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Molecular characterization of the TIA-1 like RNA-binding protein, BmTRN-1 of the silkworm, Bombyx mori.
家蚕 Bombyx mori 的 TIA-1 样 RNA 结合蛋白 BmTRN-1 的分子特征。
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[E., Matsumoto, E., Kotani, et. al.]
通讯作者:
et. al.
昆虫においてタンパク質発現を調節しているRNA結合タンパク質
调节昆虫蛋白质表达的 RNA 结合蛋白
DOI:
--
发表时间:
2006
期刊:
蚕糸昆虫バイオテック 1
影响因子:
--
作者:
[Osakabe Mh, Ohtsuka K, 小谷 英治]
通讯作者:
小谷 英治
DOI:
10.1016/j.bbaexp.2007.07.001
发表时间:
2007-09-01
期刊:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子:
--
作者:
[Tanaka, Hirornitsu, Matsuki, Hiroyuki, Yamakawa, Minoru]
通讯作者:
Yamakawa, Minoru
Nondestructive micro-patterning of proteinous occlusion bodies in water by femtosecond laser-induced mechanical forces.
通过飞秒激光诱导机械力对水中蛋白质包涵体进行无损微图案化。
DOI:
--
发表时间:
2007
期刊:
Biomed Microdevices 9
影响因子:
--
作者:
[Hosokawa, Y., Kaji, T., Shukunami, C., Hiraki, Y., Kotani, E., Mori, H., Masuhara, H.]
通讯作者:
H.
ハスモンヨトウ類細胞におけるRNA結合タンパク質(SfTRN-1)の発現解析
RNA结合蛋白(SfTRN-1)在斜纹夜蛾细胞中的表达分析
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[武藤清佳, 小谷英治, ら,]
通讯作者:
ら,
共 11 条
Molecular characterization of the TIA-1 like RNA binding protein of the silk worm, Bombyx mori.
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批准号:20580051
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.5万
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财政年份:2008
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负责人:KOTANI Eiji
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依托单位:
海外基金