Efficient carbon dioxide fixation by aromaticcarboxylate decarboxylases catalyzing carboxylation reactions

芳香族羧酸脱羧酶催化羧化反应有效固定二氧化碳

• 批准号: 18580072
• 负责人: YOSHIDA Toyokazu
• 金额: $ 2.47万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580072/
• 关键词: Enzymatic reaction; Applied microbiology; Enzyme; Bioreactor; Biological function

Various enzymes catalyzing oxidative or nonoxidative decarboxylation reaction are involved in aerobic or anaerobic degradation of aromatic carboxylic acids in microorganisms. Recently, we found and characterized several kinds of novel decarboxylase, catalyzing the reverse carboxylation reaction efficiently. In the present study, we surveyed and characterized 2,3 and 3,4 dihydroxybenzoate decarboxylases. The decarboxylases were purified to homogeneity, and physicochemicarl properties were investigated. Both dacarboxylases catalyzed the reverse carboxylation reactions for catechol as a substrate. The decarboxylases genes were cloned and analyzed. The decarboxylases were classified into independent enzyme groups on the basis of their primary structures. Furthermore, we surveyed heat stable reversible decarboxylases for the application to enzymatic carbon dioxide fixation in supercritical carbon dioxide. In supercritical carbon dioxide, pH shift and temperature affected the carboxylation activity of decarboxylases severely. From enrichment cultures using 4-hydroxybenzoic acid as a sole carbon source, microorganisms possessing heat-stable 4-hydroxybenzoate decarboxylases have been isolated.

芳香族羧酸在微生物中的好氧或厌氧降解涉及多种催化氧化或非氧化脱羧反应的酶。近年来，我们发现并鉴定了几种新型的脱羧酶，有效地催化了逆羧化反应。在本研究中，我们调查和特点的2，3和3，4二羟基苯甲酸脱羧酶。将脱羧酶纯化至均一，并研究其理化性质。这两种脱羧酶催化邻苯二酚作为底物的逆羧化反应。对脱羧酶基因进行了克隆和分析。根据一级结构将脱羧酶分为独立的酶组。此外，我们调查了热稳定可逆脱羧酶的应用，酶法二氧化碳固定在超临界二氧化碳。在超临界CO2中，pH变化和温度严重影响脱羧酶的羧化活性。从使用4-羟基苯甲酸作为唯一碳源的富集培养物中，已经分离出具有热稳定的4-羟基苯甲酸脱羧酶的微生物。

项目成果

微生物機能を活用した革新的生産技術の最前線、第II編第2章 微生物触媒によるコルベ・シュミット炭酸固定反応

利用微生物功能的创新生产技术前沿，第二卷，第2章利用微生物催化剂的科尔贝-施密特碳酸固定反应

• 发表时间: 2007
• 作者: 吉田豊和;長澤 透(分担執筆)
• 通讯作者: 長澤 透(分担執筆)

Purificationm charactetrization and gene cloning of 4-hydroxybenzoate decarboxylase of Enterobacter cloacae P240

阴沟肠杆菌P240 4-羟基苯甲酸脱羧酶的纯化、表征及基因克隆

• 发表时间: 2006
• 期刊: Archives of Microbiology 186
• 作者: T.Matsui;T.Yoshida;T.Hayashi;T.Nagasawa
• 通讯作者: T.Nagasawa

Regioselective carboxylation of 1, 3-dihydroxybenzoate by 2, 6-dihydroxybenzoate decarboxylase of Pandoraea sp. 12B-2

潘多拉菌 (Pandoraea sp.) 的 2, 6-二羟基苯甲酸脱羧酶对 1, 3-二羟基苯甲酸进行区域选择性羧化。

• 发表时间: 2006
• 期刊: Applied Microbiology and Biotechnology 73
• 作者: T. Matsui;T. Yoshida;T. Yoshimura;T. Nagasawa
• 通讯作者: T. Nagasawa

Regioselective carboxylation of 1,3-dihydroxybenzene by 2,6-dihydroxybenzoate decarboxylase of Pandoraea sp 12B-2

• DOI: 10.1007/s00253-006-0437-z
• 发表时间: 2006-11-01
• 期刊: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
• 影响因子: 5
• 作者: Matsui, Tsuyoshi;Yoshida, Toyokazu;Nagasawa, Toru
• 通讯作者: Nagasawa, Toru

2,3-ジヒドロキシ安息香酸脱炭酸酵素の炭酸固定機能の評価

2,3-二羟基苯甲酸脱羧酶的碳酸固定功能评价

• 发表时间: 2007
• 作者: T. Yoshida;et. al.;小坂拓也ら;小坂拓也;吉田 豊和;宇野 慎一
• 通讯作者: 宇野 慎一