权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mcroorgarisms composing synlrophic melhanoseric communly in paddy fieidsoil

稻田土壤中常见的共生梅尔聚糖微生物

基本信息

批准号：
18580059
负责人：
ASAKAWA Susumu
金额：
$ 2.49万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580059/
关键词：
Paddy field soil Methanoaenesis Syntroohic microbial system Cultivation method Molecular ecology Organic acid Inter-species hydrogen transfer Denaturing gradient gel electrophoresis method 酢酸プロピオン酸変性剤濃度勾配ゲル電気泳動

项目摘要

The objective of the present research project was to characterize microorganisms composing syntrophic methanogenic community in paddy field soil by the cultivation method and molecular ecological techniques. Methanogenic cultures were enriched on propionate and acetate inoculated with paddy field soil and the microbial communities in the cultures were examined. A DNA fragment closely related to Proteiniphilum acetatigenes was obtained from the culture on propionate by the denaturing gradient gel electrophoresis method. P. acetatigenes alone does not degrade propionate nor produce methane, but stimulates degradation of propionate and methanogenesis when the bacterium coexists in the propionate oxidizing syntrophic culture. The results suggested that similar syntrophic interaction may have functioned in the paddy field soil. Methanosarcina- or Methanobacterium-like methanogenic archaea and a bacterium were predominated in the culture on acetate. A hydrogenotrophic methanogenic strain, Methanobacterium sp. AH1, and a sulfate reducer, Desulfovibrio sp. Al, were isolated from the enrichment culture. Co-culture of strains AH1 and A1 degraded pyruvate, lactate and ethanol to methane, indicating syntrophic association of hydrogenotrophic methanogens and sulfate reducers for methanogenesis in paddy field soil. However, the co-culture did not degrade acetate to methane and syntrophic acetate oxidation could not be demonstrated

本研究项目的目的是通过培养方法和分子生态技术来表征稻田土壤中组成互养产甲烷群落的微生物。在接种稻田土壤的丙酸盐和乙酸盐上富集产甲烷培养物，并检查培养物中的微生物群落。通过变性梯度凝胶电泳方法从丙酸盐培养物中获得与Protehiphilum acetatigenes密切相关的DNA片段。单独的产乙酸假单胞菌不会降解丙酸盐，也不产生甲烷，但当细菌共存于丙酸盐氧化互养培养物中时，会刺激丙酸盐的降解和产甲烷作用。结果表明，类似的互养相互作用可能在稻田土壤中发挥作用。乙酸盐培养物中以甲烷八叠球菌或类甲烷杆菌和产甲烷古菌和细菌为主。一种氢营养产甲烷菌株，甲烷杆菌属。 AH1 和硫酸盐还原剂 Desulfovibrio sp。 Al，从富集培养物中分离出来。菌株 AH1 和 A1 的共培养将丙酮酸、乳酸和乙醇降解为甲烷，表明氢营养型产甲烷菌和硫酸盐还原剂在稻田土壤中产甲烷作用中存在互养关联。然而，共培养并未将乙酸盐降解为甲烷，并且无法证明互养乙酸盐氧化