Studies of nucleolin, a shuttle protein between the nucleus, cytoplasm, and cell surface

核仁素(细胞核、细胞质和细胞表面之间的穿梭蛋白)的研究

基本信息

  • 批准号:
    18590076
  • 负责人:
  • 金额:
    $ 2.52万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2006
  • 资助国家:
    日本
  • 起止时间:
    2006 至 2007
  • 项目状态:
    已结题

项目摘要

We previously found that induction of apoptosis in human Jurkat T cells results in transient capping of CD43 glycoprotein, at the stage earlier than that of phosphatidylserine exposure, and its sialylpolylactosaminyl chains serve as ligands for phagocytic recognition by macrophages. We have also shown that cell-surface nucleolin is involved in the recognition of sialylpolylactosaminyl chains cluster of early apoptotic cells.Nucleolin is a multifunctional shuttling protein present in nucleus, cytoplasm, and on the surface of some types of cells. Human nucleolin is composed of 710 amino acids and it's amino-terminal third contains alternating acidic and basic domains, its middle section contains four consensus RNA-binding domains (RBDs), and its carboxy-terminus contains a glycine/arginine-rich domain.In order to examine how cell-surface nucleolin is located on the cell surface, where it might be accessible to function as a receptor for apoptotic cells, the binding of various deletion constructs of recombinant nucleolins produced in E. coli (rNUCs) to macrophages were examined. Flowcytometric analysis using rNUCs revealed that the macrophage-binding site is located in the center region of nucleolin, containing RBDs. Previous studies suggested that some residues within the 295-302 of nucleolin are likely to be required for the recognition of early apoptotic cells.These results suggest that nucleolin binds to sialylpolylactosaminyl chains cluster on early apoptotic cells via some residues within the 295-302, whereas it interacts with macrophage via RBDs, then promoting macrophage recognition of early apoptotic cells as a bridging molecule.
我们以前发现,诱导人Jurkat T细胞凋亡的结果在短暂的封盖的CD 43糖蛋白,在比磷脂酰丝氨酸暴露的阶段,其sialylpolylactosaminyl链作为巨噬细胞的吞噬识别的配体。我们还发现细胞表面核仁素参与识别早期凋亡细胞的唾液酸聚乳糖胺链簇,核仁素是一种多功能的穿梭蛋白,存在于细胞核、细胞质和某些类型的细胞表面。人核仁素由710个氨基酸组成,其氨基端第三个结构域为酸性和碱性交替结构域,中间部分为四个共有RNA结合结构域(RBD),羧基端为富含甘氨酸/甘氨酸的结构域。E.大肠杆菌(rNUC)对巨噬细胞的作用。使用rNUCs的流式细胞术分析显示,巨噬细胞结合位点位于核仁素的中心区域,含有RBD。以往的研究表明核仁素295-302位的某些残基可能是早期凋亡细胞识别所必需的,这些结果表明核仁素通过295-302位的某些残基与早期凋亡细胞上的唾液酸聚乳糖胺链簇结合,而核仁素通过RBD与巨噬细胞相互作用,作为桥接分子促进巨噬细胞对早期凋亡细胞的识别。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Characterization of macrophage cell-surface nucleolin, a receptor for apoptotic cells
巨噬细胞表面核蛋白(凋亡细胞受体)的表征
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Yoshida;Y.;Hirano;K.;et. al.
  • 通讯作者:
    et. al.
Clearance of oxidized erythrocytes by macrophages: involvement of caspases in the generation of clearance signal at band 3 glycoprotein
巨噬细胞清除氧化红细胞:半胱天冬酶参与带 3 糖蛋白清除信号的产生
マクロファージ細胞表面ヌクレオリンの存在様式の検討
巨噬细胞表面核仁素存在方式的考察
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    吉田 靖隆、平野 和也;他
  • 通讯作者:
Clearance of oxidized erythrocytes by macrophages involvement of caspasas in the generation of clearance signal at band 3 glycoprotein
巨噬细胞清除氧化红细胞,涉及 caspasas 参与带 3 糖蛋白清除信号的产生
マクロファージ細胞表面ヌクレオリンはスカベンジャーレセプター活性を有する
巨噬细胞表面核仁素具有清道夫受体活性
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    黒田 拓也;ほか;三木雄一;久下理;鍋村実希;松島秀樹;松島 秀樹;原ゆかり;小澤大輔;原 ゆかり;松島秀樹
  • 通讯作者:
    松島秀樹
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HIRANO Kazuya其他文献

HIRANO Kazuya的其他文献

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{{ truncateString('HIRANO Kazuya', 18)}}的其他基金

REAL-TIME DETECTION OF THE EFFECTS OF ENVIRONMENTAL STRESS TO CELLULAR IMMUNITY
实时检测环境压力对细胞免疫的影响
  • 批准号:
    13672348
  • 财政年份:
    2001
  • 资助金额:
    $ 2.52万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
遺伝子発現を利用した照射効果測定
利用基因表达测量辐照效果
  • 批准号:
    11670870
  • 财政年份:
    1999
  • 资助金额:
    $ 2.52万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Identification and classification of shuttle protein in and out of nuclei
进出细胞核的穿梭蛋白的鉴定和分类
  • 批准号:
    22657036
  • 财政年份:
    2010
  • 资助金额:
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  • 项目类别:
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Arginine methylation regulates the function of a key herpesvirus nucleocytoplasmic shuttle protein
精氨酸甲基化调节关键疱疹病毒核细胞质穿梭蛋白的功能
  • 批准号:
    BB/F012101/1
  • 财政年份:
    2008
  • 资助金额:
    $ 2.52万
  • 项目类别:
    Research Grant
STUDY ON THE FUNCTIONS OF CELL-SURFACE SHUTTLE PROTEIN NUCLEOLIN
细胞表面穿梭蛋白核仁蛋白的功能研究
  • 批准号:
    19590080
  • 财政年份:
    2007
  • 资助金额:
    $ 2.52万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Retinal Shuttle Protein and Visual Photoreception
视网膜穿梭蛋白和视觉感光
  • 批准号:
    63540573
  • 财政年份:
    1988
  • 资助金额:
    $ 2.52万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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