Elucidation of the formation pathways of nitric oxide involved in the reduction of orally-ingested nitrite in vivo

在这项研究中，我们在活体实验中调查了诸如槲皮素和铁(II)等还原剂是否促进了口服亚硝酸盐形成一氧化氮(NO)气体。亚硝酸根或亚硝酸根+槲皮素组和亚硝酸铁组及亚硝酸铁组和亚硝酸铁组分别在亚硝酸根或亚硝酸铁组和亚硝酸铁组及亚硝酸铁组和亚硝酸铁组分别在亚硝酸根或亚硝酸铁组和亚硝酸铁组及亚硝酸铁组和亚硝酸铁组。有趣的是，无论是铁(II)、栎素，还是铁(II)+栎素，都不影响口服亚硝酸钠所产生的Hb^&lt；15&gt；NO总量。然而，在铁(II)或铁(II)+栎素存在的情况下，系统亚硝酸盐浓度显著降低。这些结果可能表明，在我们的实验条件下，铁(II)在亚硝酸盐生成NO气体的过程中起着至关重要的作用，而在我们的实验条件下，槲皮素在体内的贡献很小。

In this research, we investigated whether reducing agents such as quercetin and iron(II) facilitate nitric oxide (NO) gas formation from orally ingested nitrite in vivo study. When 3 mg/kg Na^<15>NO_2 was orally administered to rats with or without iron(II) or quercetin, Hb^<15>NO, which is indicative of systemic (15)^NO, was appeared in the blood, and the time to maximum blood concentration of Hb^<15>N0 was 15 min after nitrite or nitrite plus quercetin treatment, whereas nitrite plus iron(II) or nitrite plus iron(II) with quercetin shortened the time (10 min). Interestingly either iron(II), quercetin, or iron(II) plus quercetin did not affect the total amount of Hb^<15>NO generated from orally administered Na^<15>NO_2. However, systemic nitrite concentration was significantly decreased in the presence of iron(II) or iron(II) plus quercetin. These results may indicate that iron(II) is of critical importance to the generation of NO gas from nitrite, whereas quercetin provides little contribution in vivo under our experimental conditions.