Elucidation of the formation pathways of nitric oxide involved in the reduction of orally-ingested nitrite in vivo

在这项研究中，我们调查是否还原剂，如槲皮素和铁（II）促进一氧化氮（NO）气体形成从口服摄入的亚硝酸盐在体内的研究。当给大鼠口服3 mg/kg Na^ NO_2（<15>含或不含铁（II）或槲皮素）时，<15>血液中均出现代表全身性（15）^NO的Hb^ NO，<15>在亚硝酸盐或亚硝酸盐+槲皮素处理后Hb^NO达到最大血浓度的时间为15 min，而亚硝酸盐+铁（II）或亚硝酸盐+铁（II）+槲皮素处理后Hb^NO达到最大血浓度的时间缩短（10 min）。有趣的是，铁（II）、槲皮素或铁（II）加槲皮素均不影响<15>口服Na^NO_2产生的Hb^ NO总量<15>。然而，全身亚硝酸盐浓度显着降低铁（II）或铁（II）加槲皮素的存在下。这些结果可能表明，铁（II）是至关重要的NO气体从亚硝酸盐的产生，而槲皮素在我们的实验条件下，在体内提供的贡献很小。

In this research, we investigated whether reducing agents such as quercetin and iron(II) facilitate nitric oxide (NO) gas formation from orally ingested nitrite in vivo study. When 3 mg/kg Na^<15>NO_2 was orally administered to rats with or without iron(II) or quercetin, Hb^<15>NO, which is indicative of systemic (15)^NO, was appeared in the blood, and the time to maximum blood concentration of Hb^<15>N0 was 15 min after nitrite or nitrite plus quercetin treatment, whereas nitrite plus iron(II) or nitrite plus iron(II) with quercetin shortened the time (10 min). Interestingly either iron(II), quercetin, or iron(II) plus quercetin did not affect the total amount of Hb^<15>NO generated from orally administered Na^<15>NO_2. However, systemic nitrite concentration was significantly decreased in the presence of iron(II) or iron(II) plus quercetin. These results may indicate that iron(II) is of critical importance to the generation of NO gas from nitrite, whereas quercetin provides little contribution in vivo under our experimental conditions.