Elucidation of the formation pathways of nitric oxide involved in the reduction of orally-ingested nitrite in vivo

阐明体内减少口服亚硝酸盐所涉及的一氧化氮的形成途径

• 批准号: 18590235
• 负责人: TSUCHIYA Koichiro
• 金额: $ 2.23万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590235/
• 关键词: nitric oxide; quercetin; nitrite; iron; EPR; EPR; NO

In this research, we investigated whether reducing agents such as quercetin and iron(II) facilitate nitric oxide (NO) gas formation from orally ingested nitrite in vivo study. When 3 mg/kg Na^<15>NO_2 was orally administered to rats with or without iron(II) or quercetin, Hb^<15>NO, which is indicative of systemic (15)^NO, was appeared in the blood, and the time to maximum blood concentration of Hb^<15>N0 was 15 min after nitrite or nitrite plus quercetin treatment, whereas nitrite plus iron(II) or nitrite plus iron(II) with quercetin shortened the time (10 min). Interestingly either iron(II), quercetin, or iron(II) plus quercetin did not affect the total amount of Hb^<15>NO generated from orally administered Na^<15>NO_2. However, systemic nitrite concentration was significantly decreased in the presence of iron(II) or iron(II) plus quercetin. These results may indicate that iron(II) is of critical importance to the generation of NO gas from nitrite, whereas quercetin provides little contribution in vivo under our experimental conditions.

在本研究中，我们在体内研究了槲皮素和铁（II）等还原剂是否促进口服亚硝酸盐生成一氧化氮（NO）气体。大鼠口服3 mg/kg Na^<15>NO_2时，亚硝酸盐或亚硝酸盐加槲皮素处理后血中出现Hb^<15>NO，表明全身（15）^NO，且达到Hb^<15>N0血药浓度最大值的时间为15 min，而亚硝酸盐加铁（II）或亚硝酸盐加铁（II）加槲皮素使时间缩短（10 min）。有趣的是，无论是铁（II）、槲皮素，还是铁（II）加槲皮素，都不影响口服Na^<15>NO_2产生的Hb^<15>NO的总量。然而，在铁（II）或铁（II）加槲皮素的存在下，全身亚硝酸盐浓度显著降低。这些结果可能表明，在我们的实验条件下，铁（II）对亚硝酸盐生成NO气体至关重要，而槲皮素在体内的贡献很小。

项目成果

亜硝酸・硝酸由来一酸化窒素生成のケミストリー

由亚硝酸盐和硝酸生产一氧化氮的化学过程

• 发表时间: 2007
• 作者: 土屋 浩一郎

In vivo and in vitro studies on NO formation from iron-quercetin complexes

铁-槲皮素复合物形成 NO 的体内和体外研究

• 发表时间: 2007
• 作者: Xu;W.;Tanaka;K.;Sun;AQ.;You G;Shinsuke Nakagawa;K.Tsuchiya
• 通讯作者: K.Tsuchiya