Development of a in vivo screening method for evaluation prostate carcinogenic potential in chemicals

开发评估化学品中前列腺致癌潜力的体内筛选方法

• 批准号: 18590377
• 负责人: SHIRAI Tomoyuki
• 金额: $ 2.57万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590377/
• 关键词: Prostate; Carcinogen; Screening method; PhIP; DMAB; Cadmium; マイクロアレイ; oncomoduline

We obtained gene expressions profiles of rat ventral prostate tissue treated with three kinds of carcinogens, -amino-l-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP), 3, 2'-dimethyl-4-aminobiphenyl (DMAB)and cadmium. There were only three genes that was commonly up regulated in the 3 chemicals. Among the genes, we focused on oncomodulin and performed immunhistochemicl analysis for expression of oncomodulin in ventral prostates treated with the chemicals. Oncomodulin demonstrated sporadic positivity in cytoplasms of the prostate glands. However, there were no differences for expressions of oncomodulin between non-treated control group tissues and the 3 carcinogenic chemical groups, except for DMAB. There results indicated that the oncompdulin expression analysis may not be suitable for a in vivo screening method for prostate carcinogens.

我们获得了三种致癌物-氨基- 1 -甲基-6-苯基咪唑[4,5-b]吡啶（PhIP）、3,2 '-二甲基-4-氨基联苯（DMAB）和镉处理的大鼠前列腺腹侧组织的基因表达谱。只有三个基因在这三种化学物质中普遍上调。在这些基因中，我们重点研究了肿瘤调节素，并对经化学物质处理的腹侧前列腺中肿瘤调节素的表达进行了免疫组化分析。癌调节素在前列腺细胞质中呈散发阳性。而除DMAB外，肿瘤调节素在未处理对照组和3种致癌化学物质组的表达均无差异。这些结果表明，oncompdulin表达分析可能不适合用于前列腺癌物的体内筛选方法。

项目成果

Optimization of an animal test protocol for toxioogenomics studies(2);A-cross-Laboratory gene expression analysis

毒物基因组学动物试验方案的优化(2);A-跨实验室基因表达分析

• 发表时间: 2007
• 期刊: Toxicol.Sci 32
• 作者: Sumida;K.
• 通讯作者: K.

cDNAアレイを用いた前立腺発がん物質に特異的な遺伝子の検索

使用 cDNA 阵列搜索前列腺致癌物特异性基因

• 发表时间: 2008
• 作者: Tsujimura;K.;二口 充
• 通讯作者: 二口 充

Optimization of an animal test protocol for toxioogenomics studies(1);Requirement study of a protocol

毒物基因组学研究动物试验方案的优化(1)；方案要求研究

• 发表时间: 2007
• 期刊: Toxicol.Sci 32
• 作者: Sumida;K.
• 通讯作者: K.

Differences in gene expression profiles in the liver between carcinogenic and non-carcinogenic isomers of compounds given to rat in a 28-day repeat-dose toxicity study

在 28 天重复剂量毒性研究中，给予大鼠的致癌和非致癌化合物异构体在肝脏中基因表达谱的差异

• 发表时间: 2006
• 期刊: Toxicol. Appl. Pharmacol 217
• 作者: Nakayama;K.;Kawano;Y.;Kawakami;Y.;Moriwaki;N.;Masaru;S.;Otsuka;M.;Yakabe;Y.;Miyaura;H.;Saito;K.;Sumida;K.;Shirai;T
• 通讯作者: T

Prediction of carcinogenic potential by a toxicogenomic approachusingrat hepatoma cells

使用大鼠肝癌细胞通过毒理学方法预测致癌潜力

• 发表时间: 2006
• 期刊: Cancer Sci 97
• 作者: Tsujimura;K.
• 通讯作者: K.