Development of a in vivo screening method for evaluation prostate carcinogenic potential in chemicals

我们获得了三种致癌物-氨基-L-甲基-6-苯基咪唑并[4，5-b]吡啶(PhIP)、3，2‘-二甲基-4-氨基联苯(DMAB)和镉处理大鼠前列腺腹侧组织的基因表达谱。只有三个基因在这三种化学物质中普遍上调。在这些基因中，我们聚焦于肿瘤素，并对化学药物处理后的腹侧前列腺中肿瘤素的表达进行了免疫组织化学分析。肿瘤素在前列腺癌的细胞质中呈零星阳性。但除DMAB外，未处理的对照组组织与3种致癌化学物质组之间的表达差异无统计学意义。这些结果表明，癌基因表达分析可能不适用于前列腺癌的体内筛选方法。

We obtained gene expressions profiles of rat ventral prostate tissue treated with three kinds of carcinogens, -amino-l-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP), 3, 2'-dimethyl-4-aminobiphenyl (DMAB)and cadmium. There were only three genes that was commonly up regulated in the 3 chemicals. Among the genes, we focused on oncomodulin and performed immunhistochemicl analysis for expression of oncomodulin in ventral prostates treated with the chemicals. Oncomodulin demonstrated sporadic positivity in cytoplasms of the prostate glands. However, there were no differences for expressions of oncomodulin between non-treated control group tissues and the 3 carcinogenic chemical groups, except for DMAB. There results indicated that the oncompdulin expression analysis may not be suitable for a in vivo screening method for prostate carcinogens.