Analysis of the function of paramyxovaus V protein
Analysis of the function of paramyxovaus V protein
批准号:
18590448
负责人:
NISHIO Machiko
金额:
$2.55万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2006
资助国家:
日本
项目状态:
已结题
起止时间:
2006 至 2007
中文摘要
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英文摘要
1. I identified a host protein, AIPI/Alix, involved in apoptosis and efficient budding of several enveloped viruses as an interacting partner of hPIV2 NP and V proteins. To investigate possible involvement of AIPI in hPIV2 virus growth, I established cell lines in which were depleted the intracellular AIP 1 by using short hairpin RNA induced by adding Dox. The wt hPIV2 growth in the established cells was inhibited by 10- to 100-fold, but the growth rates of rPIV2s that have mutant V proteins of inability to bind with AIP I were similar. These results have shown that the association between AIPI/Alix and V proteins is important for virus growth.2. I investigated the role of the V protein in hPIV2 replication, using a mini-genome system free of vaccinia virus. I found that the C-terminal of the V protein was essential for the inhibition and for interaction with the L protein, but not for interaction with the NP protein. These data suggest that the inhibitory effect of the hPIV2 V protein is the result of L protein binding, and not that of NP.3. I found that a homogeneous set of NP, P and L proteins of PIV2 or PIV5 was able to drive reporter gene expression from the hPIV2 mini-genome system, but all heterogeneous combinations were inactive.4. By using a yeast two-hybrid-based screening assay with hPIV2 V as bait and a target cDNA library, I got some positive clones. I would like to investigate the interaction between these clones and V.
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Failure of multinucleated giant cell formation in k562 cells infected with ewcastle disease virus and human parainfluenza type 2 virus
感染新城疫病毒和人副流感2型病毒的k562细胞多核巨细胞形成失败
DOI:
--
发表时间:
2007
期刊:
Microbiol Immunol 51(6)
影响因子:
--
作者:
[Izumi, Yamakawa, et. al.]
通讯作者:
et. al.
Identifications of nuclear localization signal (NLS) on hPIV2 V protein and importin α that interacts with the V protein
hPIV2 V 蛋白上的核定位信号 (NLS) 以及与 V 蛋白相互作用的输入蛋白 α 的鉴定
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[Junbei, Ohtsuka, et. al.]
通讯作者:
et. al.
ミニゲノム系によるパラインフルエンザ2型ウイルス(hPIV2)V蛋白による転写・複製能抑制の検討
使用小基因组系统检查副流感病毒 2 型病毒 (hPIV2) V 蛋白的转录和复制能力抑制
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[Hiroshi, Komada, et. al., Machiko Nishio, Hiroshi Komada, 西尾 真智子]
通讯作者:
西尾 真智子
The interaction between the hemagglutinin-neuraminidase and fusion proteins of paramyxovirus-induced cell fusion : analysis of the interaction domain on the fusion protein by domain-swapping
副粘病毒诱导的细胞融合的血凝素-神经氨酸酶和融合蛋白之间的相互作用:通过结构域交换分析融合蛋白上的相互作用结构域
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[Masato, Tsurudome, et. al.]
通讯作者:
et. al.
Mapping of the domains on the paramyxovirus fusion protein that determine hemagglutinin-neuraminidase specificity in mediating syncytium formation
副粘病毒融合蛋白上确定介导合胞体形成的血凝素-神经氨酸酶特异性的结构域图谱
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[Masato, Tsurudome, et. al.]
通讯作者:
et. al.
共 21 条
Analysis of virus replication mechanism using minigenome or recombinant virus system
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批准号:23590539
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.33万
-
财政年份:2011
-
负责人:NISHIO Machiko
-
依托单位:
Comprehensive search and analysis of host factors that interact with the V protein of human parainfluenza virus type 2
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批准号:20590469
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.0万
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财政年份:2008
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负责人:NISHIO Machiko
-
依托单位:
Analysis of the function of hPIV2 V protein by using reverse genetics.
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批准号:15590416
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.3万
-
财政年份:2003
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负责人:NISHIO Machiko
-
依托单位:
Analysis of the nucleocapsid proteins that are constitutively expressed in cell lines
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批准号:09670312
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.05万
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财政年份:1997
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负责人:NISHIO Machiko
-
依托单位:
海外基金