权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis of phosphotyroisine-containing proteins related to the malignancy in gliomas by proteomic differential display

通过蛋白质组差异显示分析与胶质瘤恶性肿瘤相关的磷酸酪氨酸蛋白

基本信息

批准号：
18590546
负责人：
YAMAOKA Kazuko
金额：
$ 2.57万
依托单位：
Tokyo Metropolitan Organization for Medical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590546/
关键词：
glioma phosphotyroisine-containing proteins malignamcy proteome 2D-electrophoresis 二次元電気泳道

项目摘要

Gliomas are the most common of the primary intracranial tumors. Using clinically and histologically assessed various grade of gliomas, we have studied their phosphoprotein profiles by a two-dimensional gel electrophoresis-mass spectrometry approach and identified differentially expressed phosphoproteins which may be useful molecular indicators to understand these progression. Examination of the phosphoprotein profiles of glioma samples of different grades from same patient or different grades from different patients revealed about 40 distinct, differentially expressed phosphoproteins during glioma progression. These phosphoproteins of interest were picked, in-gel digested and mass spectrometry fingerprinted. Some of them were identified successfully. These phosphoproteins belonging to various functional groups such as cytoskeleton and intermediate filament proteins, heat shock proteins, enzymes and regulatory proteins. Some were found to be differentially expressed in both Grade III and glioblastoma multiform (GBM) while others were associated with a particular grade. Several proteins had been reported previously as differentially expressed in human brain cancer in non-phosphorylated form. A notable observation was that prohibitin, a potential tumor suppressor protein, and HSPs as well as glial fibrillary acidic protein (GFAP), major protein of the glial filaments were phosphorylated in both grade III and GBM tumors. We attempt to explain glioma malignancy and progression in terms of their combined role. Albumin, peroxiredoxin 1, aldolase C fructose-biphosphate, creatine kinase, hnRNPs, ATP-dependent DNA helicase were up regulated in GBM versus non-tumor tissues. These differentially expressed proteins provided novel information on the differences existing between normal brain and gliomas, and thus might prove to be useful molecular indicators of diagnostic or prognostic value.

胶质瘤是最常见的原发性颅内肿瘤。利用临床和组织学评估的不同级别的胶质瘤，我们通过二维凝胶电泳-质谱法研究了它们的磷酸化蛋白谱，并鉴定了差异表达的磷酸化蛋白，这可能是了解这些进展的有用分子指标。对同一患者不同级别或不同患者不同级别胶质瘤样本的磷酸化蛋白谱进行检查，发现胶质瘤进展过程中约有40种不同的、差异表达的磷酸化蛋白。这些感兴趣的磷蛋白被挑选出来，凝胶消化和质谱指纹图谱。其中一些已被成功识别。这些磷酸化蛋白属于各种功能基团，如细胞骨架和中间丝蛋白、热休克蛋白、酶和调节蛋白。其中一些在III级和多形性胶质母细胞瘤（GBM）中均存在差异表达，而另一些则与特定级别相关。此前有报道称，几种蛋白在人类脑癌中以非磷酸化形式差异表达。值得注意的是，在III级和GBM肿瘤中，潜在的肿瘤抑制蛋白prohibition tin、热休克蛋白（HSPs）以及胶质纤维主要蛋白胶质纤维酸性蛋白（GFAP）均被磷酸化。我们试图解释胶质瘤恶性和进展方面的综合作用。白蛋白、过氧化物还蛋白1、醛缩酶C二磷酸果糖、肌酸激酶、hnRNPs、atp依赖的DNA解旋酶在GBM中与非肿瘤组织相比上调。这些差异表达蛋白提供了正常脑和胶质瘤之间存在差异的新信息，因此可能被证明是诊断或预后价值的有用分子指标。