Role of dbpA in hepatocarcinogenesis

dbpA 在肝癌发生中的作用

• 批准号: 18590748
• 负责人: KAJINO Kazunori
• 金额: $ 2.57万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590748/
• 关键词: hepatocarcinogenesis; genomic instability; dbpA; Y box binding protein; アシルCoA合成酵素; 脂肪酸代謝

We consider dbpA to be the candidate molecule which connects the chronic inflammation and carcinogenesis. In 2006 and 2007 when we received Grant-in-aid (C), we clarified the following five points.1. Expression of dbpA in hepatocellular carcinoma (HCC) was associated with the dbpA promoter methylation in the adjacent non-HCC tissues.2. In the liver of dbpA transgenic mice, expression of cellular genes accerelating the cell proliferation was enhanced, even before the stage when the .morphological liver change occurred.3. The ischemic stress induced expression of dbpA in the liver.4. In vitro, transient overexpression of dbpA accerelated the proliferation of cultured cells.5. In the well-differentiated stomach cancer tissue, the expression of dpbA was localized in the bottom of the tubular structures. This finding suggested that, in well-differentiated stomach cancer, the dividing cells are located in the bottom of the tubular structures, as if in the bottom of crypt in normal gastric mucosa. The finding also suggested the possibility that dbpA might be the markers of canncer stem cells.

我们认为dbpA是连接慢性炎症和癌变的候选分子。在2006年和2007年接受援助款时，我们明确了以下五点。dbpA在肝细胞癌（HCC）中的表达与邻近非HCC组织中dbpA启动子甲基化相关。在dbpA转基因小鼠的肝脏中，加速细胞增殖的细胞基因表达增强，甚至在此之前。肝脏发生形态学改变。缺血应激诱导大鼠肝脏中dbpA的表达。在体外，短暂过表达dbpA可加速培养细胞的增殖。在高分化胃癌组织中，dpbA的表达定位于管状结构底部。这表明，在高分化胃癌中，分裂细胞位于管状结构的底部，就像在正常胃粘膜的隐窝底部一样。这一发现还表明，dbpA可能是癌症干细胞的标记物。

项目成果

ヒト肝癌症例におけるdbpAの発現とプロモーターメチル化による発現制御

dbpA在人肝癌中的表达及启动子甲基化的表达调控

• 发表时间: 2006
• 作者: M Yasen;梶野 一徳;有井 滋樹;樋野 興夫
• 通讯作者: 樋野 興夫

Establishment of the enzyme-linked immunosorbent assay system to detect the amino terminal secretory form of rat Erc/Mesothelin

大鼠Erc/间皮素氨基末端分泌型酶联免疫吸附测定体系的建立

• 发表时间: 2007
• 期刊: Cancer Sci 98
• 作者: Nakaishi M;Kajino K;Ikesue M;Hagiwara Y;Kuwahara M;Mitani H;Horikoshi-Sakuraba Y;Segawa T;Kon S;Maeda M;Wang T;Abe M;Yokoyama M;Hino O.
• 通讯作者: Hino O.

肝発癌過程に関与するdbpAのストレス応答蛋白としての性質

dbpA 作为参与肝癌过程的应激反应蛋白的特性

• 发表时间: 2007
• 作者: 稲見 晃一;梶野 一徳;飛田 博史;樋野 興夫
• 通讯作者: 樋野 興夫

CpG island methylation of dbpA promoter and its significance in hepatocellular carcinoma

dbpA启动子CpG岛甲基化及其在肝细胞癌中的意义

• 发表时间: 2006
• 作者: Yasen;M.;Kajino;K.;Fujii;H.;et. al.
• 通讯作者: et. al.

Gene Expression Profile of dbpA (DNA Binding Protein A) transgenic mice.

dbpA（DNA 结合蛋白 A）转基因小鼠的基因表达谱。

• 发表时间: 2005
• 期刊: Int. J. Oncology (In press)
• 作者: Tobita H.;Hino O.;et al.
• 通讯作者: et al.