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Study on the mode of human parvovirus B19 infection

人细小病毒B19感染模式的研究

基本信息

批准号：
18591194
负责人：
YOTO Yuko
金额：
$ 2.57万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Human parvovirus B19 (B19) is known as causative agents of erythema infectiosum in children and aplastic crisis in patients with congenital chronic hemolytic anemia. B19 is believed to have a single immunoserotype individuals who have suffered a disease induced by B19 aquire lifelong immunity and won't usually be reinfected. As a rare occurrence, several patients with repeated B19 infection have been reported We encountered a patient who appeared to have reinfection 3 years after the primary infection. There was no difference in genome type between the primary and the secondary infection when serum samples from our patient who seemed to have a reinfection were analyzed and compared. Latent B19 infection may persist after a primary infection. The pathological mechanism of a latent B19 infection involved in such cases will be studied There may be a special strain which predisposes to latent infections. I have examined 9,000 serum samples from 1995 to 2005. Thirty samples were positive for B19 DNA. The nudeotides sequences are now being analyzed. The association between the nucleotides and symtoms are going to be examined. After this analysis, the artificial mutation will be made in the plasmide with B19 sequences and the mode of infection will be studied by investigating for respective mRNA transcripts of B19. Now I have found the special mutation of Thymidine to Adenine at 1969nt in the case of latent infection shown above. The other analysis is now being performed.

人细小病毒B19（B19）是引起儿童感染性红斑和先天性慢性溶血性贫血患者再生障碍性危象的病原体。B19被认为具有单一的免疫血清型，患有B19诱导的疾病的个体获得终身免疫，并且通常不会再感染。作为一种罕见的发生，几个重复B19感染的患者已经报告，我们遇到了一个病人谁似乎有原发性感染后3年再感染。当分析和比较我们的患者的血清样本时，原发性和继发性感染的基因组类型没有差异，该患者似乎有再感染。潜伏性B19感染可能在原发性感染后持续存在。研究潜伏性B19感染的病理机制。可能存在一种特殊的菌株，易诱发潜伏性感染。从1995年到2005年，我检查了9,000份血清样本。30份样本B19 DNA呈阳性。目前正在分析核苷酸序列。将检查核苷酸和共辄物之间的关联。在该分析之后，将在具有B19序列的质粒中进行人工突变，并通过研究B19的相应mRNA转录物来研究感染模式。现在，我发现在上述潜伏感染的情况下，胸苷在1969 nt处突变为腺嘌呤。目前正在进行另一项分析。