The inhibition by anti-CTGF antibody produced by the DNA immunization method in the cytokine induced skin fibrosis model mice

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We established skin fibrosis animal model by subcutaneous injection of two cytokines, TGF-β and CTGF. We have conducted this project to inhibit this fibrosis model by anti-CTGF antibody.We succeeded in establishment of anti-human CTGF module 1, 2, 3 and 4 antibodies by DNA immunization method of human CTGF vector and gene gun. After the several screening studies, the anti-human CTGF module 2 antibody was revealed to be most potent of neutralizing activity.The anti-human CTGF antibody inhibited skin fibrosis dramatically, both in the early phase of TGF-β injection and the late phase of CTGF injection. In this experiment, this anti-human CTGF antibody inhibited mouse internal CTGF action.In the future, we are planning to create humanized anti-CTGF antibody for the clinical application.