The inhibition by anti-CTGF antibody produced by the DNA immunization method in the cytokine induced skin fibrosis model mice

通过皮下注射TGF-β和CTGF两种细胞因子，建立皮肤纤维化动物模型。我们通过抗ctgf抗体来抑制这种纤维化模型。我们利用人CTGF载体和基因枪的DNA免疫方法成功建立了抗人CTGF模块1、2、3和4抗体。经过多次筛选研究，发现抗人CTGF模块2抗体的中和活性最强。抗人CTGF抗体明显抑制皮肤纤维化，无论是在TGF-β注射早期，还是在CTGF注射后期。在本实验中，该抗人CTGF抗体抑制小鼠体内CTGF的作用。在未来，我们计划创建人源抗ctgf抗体用于临床应用。

We established skin fibrosis animal model by subcutaneous injection of two cytokines, TGF-β and CTGF. We have conducted this project to inhibit this fibrosis model by anti-CTGF antibody.We succeeded in establishment of anti-human CTGF module 1, 2, 3 and 4 antibodies by DNA immunization method of human CTGF vector and gene gun. After the several screening studies, the anti-human CTGF module 2 antibody was revealed to be most potent of neutralizing activity.The anti-human CTGF antibody inhibited skin fibrosis dramatically, both in the early phase of TGF-β injection and the late phase of CTGF injection. In this experiment, this anti-human CTGF antibody inhibited mouse internal CTGF action.In the future, we are planning to create humanized anti-CTGF antibody for the clinical application.