Exhaustive investigation of gene expression profile in early carcinogenesis of human lung adenocarcinoma

我们已经有了两个永生化细胞系，它们分别来自非典型腺瘤样增生（AAH）及其对应的正常支气管上皮。随着分子生物学技术的发展，在基因芯片上检测成千上万个基因的表达水平成为可能。我们的目的是利用这两个细胞系和cDNA微阵列检测在早期癌变中起重要作用的重要基因。我们检测到Sushi domain containing 2（SUSD 2）和Neuromedin U（MNU）在AAH细胞系中的过表达是正常支气管上皮细胞系的10倍以上。我们发现，SUSD 2在BAC中过表达，NMIU在早期浸润性癌中过表达。最后，我们发现SUSD 2阳性表达的小腺癌患者的生存时间较长，SUSD 2表达与较好的生存期显著相关（p=0.0112）。

We have already had two immortalized cell lines which are developed from atypical adenomatous hyperplasia (AAH) and its counterpart normal bronchial epithelium, respectively. As development of molecular biology, it was enable to detect expression levels of thousands of genes on cDNA microarray. We aimed to detect significant genes which have important roles in early carcinogenesis, using these two cell lines and cDNA microarray. We detected that Sushi domain containing 2 (SUSD2) and Neuromedin U (MNU) were overexpressed more than 10 times in AAH cell line compared with normal bronchial epithelial cell line. We revealed that SUSD2 was overexpressed in BAC and NMIU was overexpressed in early invasive cancer compared with normal lung tissue. And finally, we revealed that patients with small sized adenocarcinomas with positeve staining for SUSD2 showed long survival time, and SUSD2 expression was significantly related to preferable survival (p=0.0112).