Exhaustive investigation of gene expression profile in early carcinogenesis of human lung adenocarcinoma

人肺腺癌早期癌变过程中基因表达谱的详尽研究

• 批准号: 18591539
• 负责人: ANAMI Yoichi
• 金额: $ 2.49万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591539/
• 关键词: immortalized cell lines; atypical adenomatous hyperplasia(AAH); cDNA microarray; small adenocarcinoma; Sushi domain containing 2(SUSD2); Neuromedin U(MNU); Neuromadin U; prognostic factor; 異型腺腫様過形成(AAH)不死化細胞株; 細気管支肺胞上皮癌; 混合型腺癌; real-time PCR

We have already had two immortalized cell lines which are developed from atypical adenomatous hyperplasia (AAH) and its counterpart normal bronchial epithelium, respectively. As development of molecular biology, it was enable to detect expression levels of thousands of genes on cDNA microarray. We aimed to detect significant genes which have important roles in early carcinogenesis, using these two cell lines and cDNA microarray. We detected that Sushi domain containing 2 (SUSD2) and Neuromedin U (MNU) were overexpressed more than 10 times in AAH cell line compared with normal bronchial epithelial cell line. We revealed that SUSD2 was overexpressed in BAC and NMIU was overexpressed in early invasive cancer compared with normal lung tissue. And finally, we revealed that patients with small sized adenocarcinomas with positeve staining for SUSD2 showed long survival time, and SUSD2 expression was significantly related to preferable survival (p=0.0112).

我们已经有了两个永生化细胞系，它们分别来自非典型腺瘤样增生（AAH）及其对应的正常支气管上皮。随着分子生物学技术的发展，在基因芯片上检测成千上万个基因的表达水平成为可能。我们的目的是利用这两个细胞系和cDNA微阵列检测在早期癌变中起重要作用的重要基因。我们检测到Sushi domain containing 2（SUSD 2）和Neuromedin U（MNU）在AAH细胞系中的过表达是正常支气管上皮细胞系的10倍以上。我们发现，SUSD 2在BAC中过表达，NMIU在早期浸润性癌中过表达。最后，我们发现SUSD 2阳性表达的小腺癌患者的生存时间较长，SUSD 2表达与较好的生存期显著相关（p=0.0112）。

项目成果

Clinical values of Noguchi's classification

野口分类的临床价值

• 发表时间: 2007
• 期刊: Japan Medical Journal, Nihon Iji Shinposha
• 作者: Ohkuri T;Sato M;Abe H;Tsuji K;Yamagishi Y;Ikeda H;Matsubara N;Kitamura H;Nishimura T.;Anami Y
• 通讯作者: Anami Y

Analysis of djfferentially expressed genes in neuroendocrine carcinomas of the lung.

肺神经内分泌癌差异表达基因分析。

• 发表时间: 2007
• 期刊: Joumal of Thoracic Oncology 1
• 作者: Matsuzaki J;Tsuii T;Zhang Y;Wakita D;Imazeki I;Sakai T;Ikeda H;Nishimura T;Okubo Chigusa
• 通讯作者: Okubo Chigusa

The ACN1 gene is hypermethylated in early stage adenocarcinoma

ACN1 基因在早期腺癌中高度甲基化

• 发表时间: 2006
• 期刊: lung Journal of Thoracic Oncology 2
• 作者: Nishikawa;N;Imai;N;Ikeda;H;Kato;T;Tawara;I;Mod;K;Wang;L;Shiku;H;Shu Y
• 通讯作者: Shu Y

Identification of differentially expressed genes in the course of lung adenocarcinoma development.

肺腺癌发展过程中差异表达基因的鉴定。

• 发表时间: 2007
• 作者: 今井奈緒子;池田裕明;西川博嘉;加藤琢磨;俵功;森和子;王立楠;珠玖洋;Anami Yoichi
• 通讯作者: Anami Yoichi

検査と技術

检验与技术

• 发表时间: 2020
• 作者: So M;Miyamoto T;Murakami R;Abiko K;Hamanishi J;Baba T;Mandai M.;谷川道洋 大須賀穣
• 通讯作者: 谷川道洋 大須賀穣