Development of evaluation protocols on ecological functions of forested catchments for water quality conservation

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Achievements of this study are summarized as follows:(1) By using newly applied novel method for simultaneous measurements of N and O stable isotope ratios of NO_3^- (microbial denitrifier method), contribution and its mechanisms of atmospherically derived NO_3^- in soil nitrogen dynamics were described.(2) Stable carbon isotope ratio of dissolved organic (DOC) of soil and groundwaters were extensively surveyed, and was able to clarify the mechanisms generating the dissolved organic matter in soil profiles.(3) A long-term simulation model for nitrogen and carbon discharge from forested watersheds were developed with combining a catchment-scale biogeochemical and hydrological models. Using this model, the ecosystem functions for water quality conservation can be evaluated based on the process model for nutrient cycle in the forest ecosystem.